A5039126618- MicroRNA in disease regulation
- RNA Interference and Gene Delivery
- Circular RNAs in diseases
- Reconstructive Surgery and Microvascular Techniques
- Corneal Surgery and Treatments
- Hemoglobinopathies and Related Disorders
- Reconstructive Facial Surgery Techniques
- Antimicrobial Peptides and Activities
- Advanced biosensing and bioanalysis techniques
- DNA and Nucleic Acid Chemistry
- Periodontal Regeneration and Treatments
- Garlic and Onion Studies
- Facial Nerve Paralysis Treatment and Research
- Genomics, phytochemicals, and oxidative stress
- Nasal Surgery and Airway Studies
- Mesenchymal stem cell research
- Medical Device Sterilization and Disinfection
- Piperaceae Chemical and Biological Studies
- RNA regulation and disease
- Prenatal Screening and Diagnostics
- Tissue Engineering and Regenerative Medicine
- Cardiac Structural Anomalies and Repair
- Head and Neck Surgical Oncology
- Kruppel-like factors research
- Safe Handling of Antineoplastic Drugs
Veneto Eye Bank Foundation
2018-2025
University of Pisa
2023-2024
Ca' Foncello Hospital
2020
International Flame Research Foundation
2019
University of Ferrara
2014-2017
Section:ChooseTop of pageAbstract <<Materials and MethodsResultsDiscussionReferencesCITING ARTICLES
The biological activity of a combined treatment U251, U373 and T98G glioma cell lines with two anti-miR PNAs, directed against miR‑221 miR‑222 conjugated an ocataarginine tail (R8-PNA-a221 R8-PNA-a222) for efficient cellular delivery, was determined. Apoptosis analyzed, the effect cells either or both PNAs on reversion drug-resistance phenotype assessed in temozolomide-resistant line. Selectivity PNA/miRNA interactions studied by surface plasmon resonance (SPR)-based Biacore analysis....
Pseudomonas aeruginosa colonization, prominent inflammation with massive expression of the neutrophil chemokine IL-8, and luminal infiltrates neutrophils are hallmarks chronic lung disease in patients cystic fibrosis (CF). The nociceptive transient receptor potential ankyrin (TRPA) 1 calcium channels have been recently found to be involved nonneurogenic inflammation. Here, we investigate role TRPA1 CF respiratory inflammatory models vitro. Expression was evaluated tissue sections cells by...
The delivery of peptide nucleic acids (PNAs) to cells is a very challenging task. We report here that liposomal formulation composed egg PC/cholesterol/DSPE-PEG2000 can be loaded, according different encapsulation techniques, with PNA or fluorescent oligomers. loaded liposomes efficiently and quickly promote the uptake targeting microRNA miR-210 in human erythroleukemic K562 cells. By using this innovative system for PNA, down-regulation achieved at low concentration.
Cryopreserved homografts for valve replacement surgeries face a major problem regarding their durability after implantation and decellularized pulmonary heart valves have raised as potential new generation substitute these surgeries. The present study aims to document the work performed safe implementation in public tissue banks of decellularization method human valves, based on previous risk evaluation. After assessing preparation associated risks, using EuroGTP-II methodologies, an...
Different strategies have been proposed to target neoangiogenesis in gliomas, besides those targeting Vascular Endothelial Growth Factor (VEGF). The chemokine Interleukin-8 (IL-8) has shown possess both tumorigenic and proangiogenic properties. Although different pathways of induction IL-8 gene expression already elucidated, few data are available on its post-transcriptional regulation gliomas. Here we investigated the role microRNA miR-93 levels other pro-inflammatory genes by RT-qPCR...
Natural peptides with antimicrobial properties are deeply investigated as tools to fight bacteria resistant common antibiotics. Small peptides, those belonging the temporin family, very attractive because their activity can easily be tuned after small modification primary sequence. Structure-activity studies previously reported by us allowed identification of one peptide, analogue B, TB_KKG6A, showing, unlike against both Gram-positive and Gram-negative bacteria. In this paper, we...
Abstract The aim of this article is to report the results obtained by use HAM in surgical wound healing and reduction relapse patients affected Medication-related osteonecrosis jaw (MRONJ).The study involved with diagnosis MRONJ, surgically treated between October 2016 April 2019, a case–control setting. Enrolled were randomly divided into 2 groups. One group will be resective surgery insertion patch (Group A), while second had been exclusively B).The underwent MRONJ treatment placement...
Abstract Pericardial patches are currently used as reconstructive material in cardiac surgery for surgical treatment of septal defects. Autologous pericardial patches, either treated with glutaraldehyde or not, can be an alternative to synthetic materials xenograft congenital defects repair. The availability allogenic decellularized pericardium could reduce complication during and after a valid alternative. Decellularization tissues aims at reducing the immunogenic reaction that might...
Adipose tissue (AT) is composed of a heterogeneous population which comprises both progenitor and differentiated cells. This heterogeneity allows variety roles for the AT, including regenerative functions. In fact, autologous AT commonly used to repair soft defects, its cryopreservation could be useful strategy reduce patient discomfort caused by multiple harvesting procedures. Our work aimed characterize cryopreserved validate storage up three years clinical applications. components...
Increase of the expression γ-globin gene and high production fetal hemoglobin (HbF) in β-thalassemia patients is widely accepted as associated with a milder or even asymptomatic disease. The search for HbF-associated polymorphisms (such XmnI, BCL11A MYB polymorphisms) has recently gained great attention, order to stratify respect expectancy first transfusion, need annual intake blood, response HbF inducers (the most studied which hydroxyurea). Aγ-globin sequencing was performed on genomic...
Osteoarthritis (OA) is a chronic debilitating disorder causing pain and gradual degeneration of joints. Among various cell therapies, mesenchymal stem (MSC) therapy appears to provide encouraging results. Human amniotic suspension allografts (HASA) have anti-inflammatory chondroregenerative potential represent promising treatment strategy. The purpose the present study was prospectively assess safety, clinical effectiveness, feasibility intra-articular injections human allograft in...
Adoptive immunotherapy with Cytokine Induced Killer (CIK) cells has shown antitumor activity against several kinds of cancers in preclinical models and clinical trials. CIK are a subset ex vivo expanded T lymphocytes T-NK phenotype MHC-unrestricted activity. Literature provides scanty information on cytokines, chemokines growth factors secreted by cells. Therefore, we investigated the secretory profile generated from tumor patients. The secretome analysis was performed at specific time...