A5017854085- Adenosine and Purinergic Signaling
- Adolescent and Pediatric Healthcare
- Neuroscience of respiration and sleep
- Ion channel regulation and function
- Immune Cell Function and Interaction
- RNA Interference and Gene Delivery
- Neuroscience and Neuropharmacology Research
- Neurological Complications and Syndromes
- COVID-19 Impact on Reproduction
- Inflammasome and immune disorders
- Mast cells and histamine
- Neuroendocrine Tumor Research Advances
- Calcium signaling and nucleotide metabolism
- Neuroinflammation and Neurodegeneration Mechanisms
- Vagus Nerve Stimulation Research
- Cancer Immunotherapy and Biomarkers
- Pharmacological Receptor Mechanisms and Effects
- Receptor Mechanisms and Signaling
- Cell death mechanisms and regulation
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Tryptophan and brain disorders
- Pregnancy and Medication Impact
- Neonatal Respiratory Health Research
- Lipid Membrane Structure and Behavior
- Macrophage Migration Inhibitory Factor
University of Ferrara
2016-2025
University of Rome Tor Vergata
2025
Istituti di Ricovero e Cura a Carattere Scientifico
2002-2025
Vita-Salute San Raffaele University
2024-2025
Istituto di Ricovero e Cura a Carattere Scientifico San Raffaele
2024
University of Florence
2022-2023
Google (United States)
2013
Inserm
2012
Sorbonne Paris Cité
2012
Hôpital Européen Georges-Pompidou
2012
Antineoplastic chemotherapies are particularly efficient when they elicit immunogenic cell death, thus provoking an anticancer immune response. Here we demonstrate that autophagy, which is often disabled in cancer, dispensable for chemotherapy-induced death but required its immunogenicity. In response to chemotherapy, autophagy-competent, not autophagy-deficient, cancers attracted dendritic cells and T lymphocytes into the tumor bed. Suppression of autophagy inhibited release adenosine...
Background There is growing awareness that tumour cells build up a “self-advantageous” microenvironment reduces effectiveness of anti-tumour immune response. While many different immunosuppressive mechanisms are likely to come into play, recent evidence suggests extracellular adenosine acting at A2A receptors may have major role in down-modulating the response as cancerous tissues contain elevated levels and break-down products. there no doubt all possess plasma membrane transporters mediate...
Abstract Extracellular ATP (ATPe) is known to cause release of processed IL-1 beta from LPS-treated macrophages and microglial cells. fast thought be associated with cell death. We have reinvestigated this process identify 1) the purinergic receptor involved; 2) relationship death; 3) pharmacologic agonists or antagonists able modulate release. Our data confirm that ATPe a powerful stimulus for human macrophages; however, we also show not necessarily death, as it occurs at lower...
The intracellularly trappable fluorescent Ca2+ indicator quin-2 was used to measure free cytosolic Ca2+, [Ca2+]i, in the two highly dedifferentiated tumor cell lines, Ehrlich and Yoshida ascites carcinomas. It found that these carcinoma cells can trap similarly normal cells, but [Ca2+]i apparently significantly lower than any tested previously with this method. By using a new lipid-soluble heavy metal chelator TPEN (N,N,N',N'-tetrakis(2-pyridylmethyl)ethylenediamine), which crosses...
Microglial cells express a peculiar plasma membrane receptor for extracellular ATP, named P2Z/P2X7 purinergic receptor, that triggers massive transmembrane ion fluxes and reversible permeabilization of the to hydrophylic molecules up 900 dalton molecule weight eventual cell death (Di Virgilio, F. 1995. Immunol. Today. 16:524–528). The physiological role this newly cloned (Surprenant, A., Rassendren, E. Kawashima, R.A. North G. Buell. 1996. Science (Wash. DC). 272:735–737) cytolytic is...
The mechanism of action the oncogene bcl-2, a key regulator apoptotic process, is still debated. We have employed organelle-targeted chimeras Ca2+-sensitive photoprotein, aequorin, to investigate in detail effect Bcl-2 overexpression on intracellular Ca2+ homeostasis. In ER and Golgi apparatus, increases leak (while leaving accumulation unaffected), hence reducing steady-state [Ca2+] levels. As direct consequence, caused by inositol 1,4,5 trisphosphate (IP3)-generating agonists were reduced...
The P2X7 receptor is an ATP-gated ion channel known for its cytotoxic activity. However, recent evidence suggests a role in cell proliferation. Here, we found that exhibits significant growth-promoting effects vivo. Human embryonic kidney cells expressing exhibited more tumorigenic and anaplastic phenotype than control vivo, the growth rate size of these tumors were significantly reduced by intratumoral injection inhibitor-oxidized ATP. accelerated P2X7-expressing was characterized increased...
Significance Accumulating evidence has underscored the role of cytosolic p53 in promoting cell death. Different reports have revealed that participates apoptosis induction by acting directly at mitochondria. However, because can mediate without its DNA-binding domain (the proposed to be fundamental for targeting mitochondria), mitochondrial localization is likely not only transcription-independent mechanism which promotes apoptosis. Here we demonstrate endoplasmic reticulum (ER) and...