A5040678382- Amyotrophic Lateral Sclerosis Research
- Alzheimer's disease research and treatments
- Neuroinflammation and Neurodegeneration Mechanisms
- Neuroscience and Neuropharmacology Research
- Neurogenetic and Muscular Disorders Research
- Mitochondrial Function and Pathology
- RNA Interference and Gene Delivery
- DNA and Nucleic Acid Chemistry
- Carcinogens and Genotoxicity Assessment
- Nerve injury and regeneration
- Neurological diseases and metabolism
- DNA Repair Mechanisms
- Microbial Metabolic Engineering and Bioproduction
- Neurogenesis and neuroplasticity mechanisms
- Prion Diseases and Protein Misfolding
- Biofuel production and bioconversion
- Peroxisome Proliferator-Activated Receptors
- Genetics and Neurodevelopmental Disorders
- Parkinson's Disease Mechanisms and Treatments
- ATP Synthase and ATPases Research
- Ion channel regulation and function
- Metabolism and Genetic Disorders
- Redox biology and oxidative stress
- Fungal and yeast genetics research
- Skin Diseases and Diabetes
Istituti Clinici Scientifici Maugeri
2017-2024
Fondazione Salvatore Maugeri
2011-2016
University of Milano-Bicocca
2010
University of Milan
1990-2008
University of Urbino
1996-1999
University of Pavia
1995
ATP, released by both neurons and glia, is an important mediator of brain intercellular communication. We find that selective activation purinergic P2Y1 receptors (P2Y1R) in cultured astrocytes triggers glutamate release. By total internal fluorescence reflection imaging fluorescence-labeled glutamatergic vesicles, we document such release occurs regulated exocytosis. The stimulus-secretion coupling mechanism involves Ca2+ from stores controlled additional transductive events mediated tumor...
Collective evidence indicates that motor neuron degeneration in amyotrophic lateral sclerosis (ALS) is non-cell-autonomous and requires the interaction with neighboring astrocytes. Recently, we reported a subpopulation of spinal cord astrocytes degenerates microenvironment neurons hSOD1G93A mouse model ALS. Mechanistic studies vitro identified role for excitatory amino acid glutamate gliodegenerative process via activation its inositol 1,4,5-triphosphate (IP3)-generating metabotropic...
Identification of reliable and accessible biomarkers to characterize ischemic stroke patients’ prognosis remains a clinical challenge. Neurofilament light chain (NfL) glial fibrillary acidic protein (GFAP) are markers brain injury, detectable in blood by high-sensitive technologies. Our aim was measure serum NfL GFAP after stroke, evaluate their correlation with functional outcome the scores rehabilitation scales at 3-month follow-up. Stroke patients were prospectively enrolled longitudinal...
Considerable evidence indicates that neurodegeneration in amyotrophic lateral sclerosis (ALS) can be conditioned by a deleterious interplay between motor neurons and astrocytes. Astrocytes are the major glial component central nervous system (CNS) fulfill several activities essential to preserve CNS homeostasis. In physiological pathological conditions, astrocytes secrete wide range of factors which they exert multimodal influences on their cellular neighbours. Among others, cell...
Several lines of evidence suggest that astrocytes play a key role in modulating the immune responses central nervous system (CNS) to infections, injuries, or pathologies. Yet, their contribution these processes remains mostly elusive. Astroglia are endowed with wide range toll-like receptors (TLR) by which they can sense infectious agents as well endogenous danger signals released damaged cells. Here we demonstrate activation astrocytic TLR4 bacterial lipopolysaccharide (LPS) challenge...
The current inability of clinical criteria to accurately identify the "at-risk group" for Amyotrophic Lateral Sclerosis (ALS) development as well its unknown etiology are fueling interest in biomarkers aimed at completing approaches diagnosis. Glial cell line-derived neurotrophic factor (GDNF) is a diffusible peptide critically involved neuronal differentiation and survival. GDNF largely studied various neurological neuromuscular diseases, with great peripheral nervous system (PNS). recent...
Pharmacologically attainable concentrations of ascorbic acid are highly toxic for U937 cells (a human promyelocytic cell line), and this response appears to be mediated by H2O2. This inference finds experimental support in the following observations: 1) levels H2O2 readily generated upon dissolution survival-range culture medium; 2) lethal effects elicited or reagent prevented addition either catalase intracellular iron chelator o-phenanthroline characterized similar temporal dependence; 3)...
The cytokine tumor necrosis factor-alpha (TNFalpha) induces Ca2+-dependent glutamate release from astrocytes via the downstream action of prostaglandin (PG) E2. By this process, may participate in intercellular communication and neuromodulation. Acute inflammation vitro, induced by adding reactive microglia to astrocyte cultures, enhances TNFalpha production amplifies release, switching pathway into a neurodamaging cascade (Bezzi, P., Domercq, M., Brambilla, L., Galli, R., Schols, D., De...
Astrocytes have emerged as critical elements for the maintenance and function of central nervous system. The expression on their cell membrane RAGE TLR4 receptors makes astrocytes susceptible to High-Mobility Group Box 1 (HMGB1), a nuclear protein typically released in extracellular milieu by living cells experiencing physiological stress conditions or damaged cells. Here, we show that interaction HMGB1 with normal spinal cord induces astrocytic production neurotrophic factors, particularly...