A5021474771- Gold and Silver Nanoparticles Synthesis and Applications
- Advanced biosensing and bioanalysis techniques
- Nanoparticle-Based Drug Delivery
- Nanoparticles: synthesis and applications
- Nanocluster Synthesis and Applications
- RNA Interference and Gene Delivery
- Advanced Nanomaterials in Catalysis
- Nanomaterials for catalytic reactions
- Biosensors and Analytical Detection
- Boron Compounds in Chemistry
- Polymer Surface Interaction Studies
- Catalytic Processes in Materials Science
- Nanoplatforms for cancer theranostics
- Protein Interaction Studies and Fluorescence Analysis
- Animal Virus Infections Studies
- Electrostatics and Colloid Interactions
- Lanthanide and Transition Metal Complexes
- Field-Flow Fractionation Techniques
- Healthcare cost, quality, practices
- Molecular Communication and Nanonetworks
- COVID-19 and healthcare impacts
- Phytochemistry and biological activity of medicinal plants
- CO2 Reduction Techniques and Catalysts
- Virology and Viral Diseases
- Lipid Membrane Structure and Behavior
University of Salford
2017-2024
National Physical Laboratory
2022
Korea Research Institute of Standards and Science
2022
University College Dublin
2014-2017
University of Strathclyde
2012-2014
University of Liverpool
2009-2014
University of Milan
2008-2010
National Interuniversity Consortium of Materials Science and Technology
2007
Abstract The exploration of the genetic information carried by DNA has become a major scientific challenge. Routine analysis, such as PCR, still suffers from important intrinsic limitations. Surface‐enhanced Raman spectroscopy (SERS) emerged an outstanding opportunity for development but its application to duplexes (dsDNA) been largely hampered reproducibility and/or sensitivity issues. A simple strategy is presented perform ultrasensitive direct label‐free analysis unmodified dsDNA with...
The transport and the delivery of drugs through nanocarriers is a great challenge pharmacology. Since production liposomes to reduce toxicity doxorubicin in patients, plethora nanomaterials have been produced characterized. Although it widely known that elementary properties influence their vivo kinetics, such interaction often poorly investigated many preclinical studies. present study aims evaluate actual effect size shape on biodistribution set gold nanoparticles (GNPs) after intravenous...
A simple single-phase method for the preparation of ca. 2 nm gold nanoparticles capped with mercaptocarborane ligands is introduced. The resultant monolayer protected clusters (MPCs) exhibit redox-dependent solubility and readily phase transfer between water nonpolar solvents depending on electronic ionic charge stored in metal core ligand shell, respectively. particles their properties have been characterized by high angle annular dark field imaging a scanning transmission electron...
This paper contributes to the debate on how nanosized objects negotiate membrane barriers inside biological cells. The uptake of peptide-modified gold nanoparticles by HeLa cells has been quantified using atomic emission spectroscopy. TAT peptide from HIV virus was singled out as a particularly effective promoter cellular uptake. evolution intracellular distribution TAT-modified with time studied in detail TEM and systematic image analysis. An unusual trend particles disappearing cytosol...
Here we report on the design, synthesis and application of small, highly bright, star-shaped SERS encoded single nanoparticles with ability providing an optical signal upon excitation near infrared light. These particles are colloidally stable, fully biocompatible can be internalized into living cells for intracellular imaging.
Abstract Colorimetric detection of analytes using gold nanoparticles along with surface‐enhanced Raman spectroscopy (SERS) are areas intense research activity since they both offer sensing very low concentrations target species. Multimodal promotes the simultaneous a sample by combination different techniques; consequently, surface chemistry design in development multimodal nanosensors is important for rapid and sensitive evaluation diverse analytical methods. Herein it shown that...
We show that low-intensity laser irradiation of cancer cells containing endosomal gold nanoparticles leads to endosome rupture and escape the into cytosol without affecting cells' viability. The low light intensity our experiments allows us rule out photothermal effects as underlying mechanism, we present results suggest photoinduced radicals photogenerated active species. This nonthermal mechanism may also be important in context cell death at higher intensities, which had been reported previously.
The standard protocols for DNA analysis largely involve polymerase chain reaction (PCR). However, structures bound to chemical agents cannot be PCR-amplified, and therefore any sequence changes induced by external may neglected. Thus, the development of analytical tools capable characterizing biochemical mechanisms associated with chemically damage is demanded rational design more effective chemotherapy drugs, understanding mode actions carcinogenic chemicals, monitoring genotypic toxicology...
Improved positively charged nanoparticles are described to provide a simplified SERS substrate for DNA detection. Complete flocculation of the is prevented due controlled analyte induced aggregation. This provides stable aggregation state which significantly extends analysis window simplifying detection by SERS.
Differential centrifugal sedimentation (DCS) has been applied to accurately size ligand-protected gold hydrosols in the 10 50 nm range. A simple protocol is presented correct for particle density variations due presence of ligand shell, which formed here by either polyethylene glycol-substituted alkane thiols (PEG-alkane thiols) different chain length or oligopeptides. The method gives reliable data all sizes investigated and lends itself rapid routine sizing nanoparticles. Unlike TEM, DCS...
Abstract The exploration of the genetic information carried by DNA has become a major scientific challenge. Routine analysis, such as PCR, still suffers from important intrinsic limitations. Surface‐enhanced Raman spectroscopy (SERS) emerged an outstanding opportunity for development but its application to duplexes (dsDNA) been largely hampered reproducibility and/or sensitivity issues. A simple strategy is presented perform ultrasensitive direct label‐free analysis unmodified dsDNA with...
Gold nanoparticles of two different sizes stabilized by a 15-mer peptide ligand specifically designed for this purpose have been prepared in aqueous solution and characterized UV−vis spectroscopy TEM. The presence the its binding mode to particles via four cystein thiols is evidenced FTIR NMR spectroscopy. Biotinylation freely accessible lysine residue demonstrated.
The range of possible nanostructures is so large and continuously growing, that collating unifying the knowledge connected to them, including their biological activity, a major challenge. Here we discuss concept based on connection microscopic features nanomaterials impacts. We also consider what would be necessary identify control interactions, make them resemble each other in context.
Self-assembling protein nanoparticles are used as a novel vaccine design platform to improve the stability and immunogenicity of safe subunit vaccines, while providing broader protection against viral infections. Infectious Hematopoietic Necrosis virus (IHNV) is causative agent WOAH-listed IHN diseases for which there currently no therapeutic treatments globally available commercial vaccine. In this study, by genetically fusing glycoprotein H. pylori ferritin scaffold, we constructed...
We report the evidence for cellular uptake of gold nanoparticles via phagocytosis mechanism in murine macrophage cells strongly supported by TEM and optical microscopy. Nanoparticles were prepared using several biocompatible molecules choice (5-aminovaleric acid, l-DOPA, melatonin, serotonin hydrochloride) as stabilizers colloids. Their surface chemistry was fully characterized UV−vis, ATR-FTIR, 1H NMR, HR-MAS NMR spectroscopies, size distribution determined CPS disc centrifuge TEM....
Gold nanoparticles (GNPs) are of considerable interest for use as a radiosensitizer, because their biocompatibility and ability to increase dose deposited high mass energy absorption coefficient. Their sensitizing properties have been verified experimentally, but discrepancy between the experimental results theoretical predictions suggests that effect does not depend solely on gold's superior energetic photons. This work presents three sets experiments independently mapped out dependence...
Abstract Advances in nanofabrication methods have enabled the tailoring of new strategies towards controlled production nanoparticles with attractive applications healthcare. In many cases, their characterisation remains a big challenge, particularly for small-sized functional 5 nm diameter or smaller, where current particle sizing techniques struggle to provide required sensitivity and accuracy. There is clear need development reliable approaches physico-chemical significant accuracy,...
We describe the outcome of a large international interlaboratory study measurement particle number concentration colloidal nanoparticles, project 10 technical working area 34, "Nanoparticle Populations" Versailles Project on Advanced Materials and Standards (VAMAS). A total 50 laboratories delivered results for 30 nm gold nanoparticles measured using tracking analysis (PTA), single inductively coupled plasma mass spectrometry (spICP-MS), ultraviolet-visible (UV-Vis) light spectroscopy,...
Traditional methods for the construction of nanoparticle arrays and lattices exploit Watson-Crick base pairing single-stranded DNA sequences as a proxy self-assembly. Although this approach has been utilized in variety applications nanoassembly, diagnostics, biomedicine, diversity recognition lexicon could be considerably increased by developing strategies that recognize base-pairing landscape double-stranded (dsDNA) sequences. Herein we describe first report programmed gold (GNP)...
Single particle extinction and scattering (SPES) is used to detect the shape of anisotropic gold nanoparticles in dispersion <italic>in situ.</italic>
Abstract A preliminary investigation on a new class electrocatalytic materials for the electroreduction of organic halides is presented and discussed. The electrocatalysts are based silver nanoparticles (Ag_NP), ad hoc synthesised by chemical reduction an aqueous salt in presence six different stabilising agents. colloids then supported carbon powder (10% loading) further characterisation use. properties Ag_NP/carbon composites towards dehalogenation halocompounds tested cyclic voltammetry...