A5006504140- Nanoparticle-Based Drug Delivery
- Gold and Silver Nanoparticles Synthesis and Applications
- Nanoplatforms for cancer theranostics
- Advanced biosensing and bioanalysis techniques
- Nanoparticles: synthesis and applications
- Advanced Nanomaterials in Catalysis
- Metal-Organic Frameworks: Synthesis and Applications
- Acute Ischemic Stroke Management
- Nanocluster Synthesis and Applications
- Biosensors and Analytical Detection
- RNA Interference and Gene Delivery
- Dendrimers and Hyperbranched Polymers
- Graphene and Nanomaterials Applications
- Advanced Biosensing Techniques and Applications
- Force Microscopy Techniques and Applications
- Neuroinflammation and Neurodegeneration Mechanisms
- Bacteriophages and microbial interactions
- Monoclonal and Polyclonal Antibodies Research
- Photoacoustic and Ultrasonic Imaging
- Extracellular vesicles in disease
- Polymer Surface Interaction Studies
- Advanced Proteomics Techniques and Applications
- Mechanical and Optical Resonators
- Covalent Organic Framework Applications
- Electrospun Nanofibers in Biomedical Applications
Center for Research in Molecular Medicine and Chronic Diseases
2018-2025
Universidade de Santiago de Compostela
2018-2025
Centro Singular de Investigación en Química Biológica y Materiales Moleculares
2024
University College Dublin
2014-2018
22q11 Ireland
2018
Universidad de Zaragoza
2011-2014
Instituto de Nanociencia y Materiales de Aragón
2011
Biomolecules adsorbed on nanoparticles are known to confer a biological identity nanoparticles, mediating the interactions with cells and barriers. However, how these molecules presented particle surface in milieu remains unclear. The central aim of this study is identify key protein recognition motifs link them specific cell-receptor interactions. Here, we employed an immuno-mapping technique quantify epitope presentations two major proteins serum corona, low-density lipoprotein...
Abstract Nanoparticles interacting with, or derived from, living organisms are almost invariably coated in a variety of biomolecules presented complex biological milieu, which produce bio-interface ‘biomolecular corona’ conferring identity to the particle. Biomolecules at surface nanoparticle–biomolecule present molecular fragments that may be recognized by receptors cells barriers, potentially engaging with different pathways. Here we demonstrate using intense fluorescent reporter binders,...
Several strategies for linking antibodies (Abs) through their Fc region in an oriented manner have been proposed at the present time. By using these strategies, Fab of Ab is available antigen molecular recognition, leading to a more efficient interaction. Most are complex processes optimized mainly functionalization surfaces or microbeads. These methodologies imply though modification several steps purification use expensive immobilized proteins. Besides, magnetic nanoparticles (MNPs) turned...
Abstract A plasmonic core–shell gold nanostar/zeolitic‐imidazolate‐framework‐8 (ZIF‐8) nanocomposite was developed for the thermoplasmonic‐driven release of encapsulated active molecules inside living cells. The nanocomposites were loaded, as a proof concept, with bisbenzimide functional cargo and wrapped an amphiphilic polymer that prevents ZIF‐8 degradation leaking in aqueous media or demonstrated molecule‐release mechanism relies on use near‐IR light coupled to absorption core nanostars,...
In this Perspective, we describe current challenges and recent advances in efficient delivery targeting of nanoparticles vivo. We discuss cancer therapy, nanoparticle-biomolecule interactions, nanoparticle trafficking cells, triggers responses to nanoparticle-cell interactions. No matter which functionalization strategy target is chosen, passive or active targeting, more than 99% the administered vivo end up mononuclear phagocytic system, mainly sequestered by macrophages. Comprehensive...
Characterizing the orientation of covalently conjugated proteins on nanoparticles, produced for in vitro and vivo targeting, though an important feature such a system, has proved challenging. Although extensive physicochemical characterization targeting nanoparticles can be addressed detail, relevant biological nanointerface is crucial order to select suitable nanomaterials further or experiments. In this work, we adopt methodology using antibody fragments (Fab) gold (immunogold) map...
Abstract Ultrasmall nanoparticles (USNPs), usually defined as NPs with core in the size range 1–3 nm, are a class of nanomaterials which show unique physicochemical properties, often different from larger same material. Moreover, there also indications that USNPs might have distinct properties their biological interactions. For example, recent vivo experiments suggest some escape liver, spleen, and kidney, contrast to strongly accumulated liver. Here, we present simple approach study...
The systematic study of nanoparticle-biological interactions requires particles to be reproducibly dispersed in relevant fluids along with further development the identification biologically structural details at materials-biology interface. Here, we develop a biocompatible long-term colloidally stable water dispersion few-layered graphene nanoflakes biological exposure medium which it will studied. We also report orientation and functionality key proteins interest biolayer (corona) that are...
Highly homogenous nanoparticles fabricated via nanoimprint lithography with minimal batch-to-batch size variation and tailor-made plasmonic properties. Polymer surface modification ensures functionality, good biocompatibility minor cytotoxicity.
Herein, we describe the development of a new, highly efficient label for immunosensing comprising an antibody/PEGylated gold-nanoparticle (AuNP) conjugate in which antibody molecules are bound to AuNP surface fixed orientation. Our method exploits high density positive charges on major plane antibodies that exists when pH solution is lower than isoelectric point antibody; interact with negatively charged through their plane, enabling antigen binding sites move freely and therefore reach...
Intravenous administration of fibrinolytic drugs, such as recombinant tissue plasminogen activator (rtPA) is the standard treatment acute thrombotic diseases. However, current fibrinolytics exhibit limited clinical efficacy because their short plasma half-lives and risk hemorrhagic transformations. Platelet membrane-based nanocarriers have received increasing attention for ischemic stroke therapies, they natural thrombus-targeting activity, can prolong half-life therapy, reduce side effects....
Nanoenabled technology holds great potential for health issues and biological research. Among the numerous inorganic nanoparticles that are available today, gold fully developed as therapeutic diagnostic agents both in vitro vivo due to their physicochemical properties. Owing this, substantial work has been conducted terms of developing biosensors noninvasive targeted tumor diagnosis treatment. Some studies have even expanded into clinical trials. This article focuses on fundamentals...
The range of possible nanostructures is so large and continuously growing, that collating unifying the knowledge connected to them, including their biological activity, a major challenge. Here we discuss concept based on connection microscopic features nanomaterials impacts. We also consider what would be necessary identify control interactions, make them resemble each other in context.
Nanoscale metal-organic frameworks (NMOFs) exhibit unique properties for drug delivery, including ultrahigh storage capabilities, biocompatibility, biodegradability, and sustained release of encapsulated cargo. However, due to their localized electronic states, MOFs are nonresponsive external stimuli such as light or magnetic fields. This study investigates the integration light-responsive nanoantennas into NMOFs enhance application smart delivery nanosystems. By integrating gold bipyramid...
This communication describes an ultrafast high-sensitivity plasmonic-driven thermal sensing approach for the detection of tumoral markers. Gold nanoprisms derivatized with antibody against carcinoembryonic antigen (anti-CEA) are used to label CEA molecules and imprint signals upon illumination, enabling sensitivities up attomolar range in real patient samples.
Small interfering RNA (siRNA) is a promising molecule for gene therapy, but its therapeutic administration remains problematic. Among the recently proposed vectors, cell-penetrating peptides show great promise in vivo trials siRNA delivery. Human protein DMBT1 (deleted malignant brain tumor 1) pattern recognition that interacts with polyanions and recognizes aggregates bacteria. Taking advantage of these properties, we investigated whether specific synthetic DMBT1-derived could be used to...
We present a fast, label-free, QCM-based methodology for the screening of accessible protein recognition epitopes on NP biological surface.
We describe a microporous plasmonic nanoreactor to carry out designed near-infrared (NIR)-driven photothermal cyclizations inside living cells. As proof of concept, we chose an intramolecular cyclization that is based on the nucleophilic attack pyridine onto electrophilic carbon, process requires high activation energies and typically achieved in bulk solution by heating at ∼90 °C. The core–shell (NR) has been include gold nanostar core, which embedded within metal–organic framework (MOF)...