A5100718883- Nanoparticle-Based Drug Delivery
- Polymer Surface Interaction Studies
- RNA Interference and Gene Delivery
- Crystallization and Solubility Studies
- X-ray Diffraction in Crystallography
- Advanced biosensing and bioanalysis techniques
- Nanoplatforms for cancer theranostics
- Immunotherapy and Immune Responses
- Nanofabrication and Lithography Techniques
- Minerals Flotation and Separation Techniques
- 3D Printing in Biomedical Research
- Electrospun Nanofibers in Biomedical Applications
- biodegradable polymer synthesis and properties
- Metal Extraction and Bioleaching
- Graphene and Nanomaterials Applications
- Dendrimers and Hyperbranched Polymers
- Nanoparticles: synthesis and applications
- Diamond and Carbon-based Materials Research
- Extraction and Separation Processes
- Hydrogels: synthesis, properties, applications
- RNA modifications and cancer
- Combustion and flame dynamics
- Advanced Polymer Synthesis and Characterization
- Molecular Sensors and Ion Detection
- Gold and Silver Nanoparticles Synthesis and Applications
University College Dublin
2016-2025
Nanjing University
2016-2025
Nanjing General Hospital of Nanjing Military Command
2016-2025
Commercial Aircraft Corporation of China (China)
2025
Xihua University
2024-2025
Center for Interdisciplinary Studies of Molecular Interactions
2025
Conway School of Landscape Design
2025
Yangzhou University
2025
The First Affiliated Hospital, Sun Yat-sen University
2024
Zhongshan Hospital
2024
Metal–organic coordination materials are of widespread interest because the coupled benefits inorganic and organic building blocks. These can be assembled into hollow capsules with a range properties, which include selective permeability, enhanced mechanical/thermal stability, stimuli-responsiveness. Previous studies have primarily focused on assembly aspects metal-coordination capsules; however, engineering metal-specific functionality for capsule design has not been explored. A library...
Stable polydopamine capsules are prepared by the single-step deposition and self-polymerization of dopamine onto a range colloidal silica template particles with different sizes porosities. MTT assays reveal negligible cytotoxicity toward cells.
We report a facile approach to immobilize pH-cleavable polymer-drug conjugates in mussel-inspired polydopamine (PDA) capsules for intracellular drug delivery. Our design takes advantage of the PDA coating form capsules, chemical reactivity films, and acid-labile groups polymer side chains sustained pH-induced release. The anticancer doxorubicin (Dox) was conjugated thiolated poly(methacrylic acid) (PMASH) with hydrazone bond, then immobilized via robust thiol-catechol reactions between...
Many biomolecules, mainly proteins, adsorb onto polymer particles to form a dynamic protein corona in biological environments. The can significantly influence particle–cell interactions, including internalization and pathway activation. In this work, we demonstrate the differential roles of given formed cell culture media particle uptake by monocytes macrophages. By exposing disulfide-stabilized poly(methacrylic acid) nanoporous (PMASH NPPs) complete growth containing 10% fetal bovine serum,...
Biomolecules adsorbed on nanoparticles are known to confer a biological identity nanoparticles, mediating the interactions with cells and barriers. However, how these molecules presented particle surface in milieu remains unclear. The central aim of this study is identify key protein recognition motifs link them specific cell-receptor interactions. Here, we employed an immuno-mapping technique quantify epitope presentations two major proteins serum corona, low-density lipoprotein...
The significance of a protein corona on nanoparticles in modulating particle properties and their biological interactions has been widely acknowledged. is derived from proteins fluids, many which are glycosylated. To date, the glycans have largely overlooked studies nanoparticle–cell interactions. In this study, we demonstrate that glycosylation plays an important role maintaining colloidal stability influences removal enhances cell membrane adhesion uptake comparison with fully glycosylated...
Water-insoluble compounds were encapsulated in polymer capsules through mesoporous silica nanoparticle-mediated layer-by-layer assembly. The drug-loaded exhibit excellent colloidal stability and high potency to colorectal cancer cells vitro with similar cytotoxicity the free drug dissolved organic solvent. Detailed facts of importance specialist readers are published as ”Supporting Information”. Such documents peer-reviewed, but not copy-edited or typeset. They made available submitted by...
Multilayered polymer particles with a wide range of properties can be precisely engineered via the layer-by-layer technique using multitude templates and materials. Such multilayered are emerging as powerful platform for biomedical applications. In this short review, we provide an overview developments in engineering particles, describe recent progress their utilization biological systems. Emphasis is placed on strategies that achieve different objectives, ranging from triggered...
We report the modular assembly of a polymer−drug conjugate into covalently stabilized, responsive, biodegradable, and drug-loaded capsules with control over drug dose position in multilayer film. The cancer therapeutic, doxorubicin hydrochloride (DOX), was conjugated to alkyne-functionalized poly(l-glutamic acid) (PGAAlk) via amide bond formation. PGAAlk PGAAlk+DOX were assembled hydrogen bonding poly(N-vinyl pyrrolidone) (PVPON) on planar colloidal silica templates. films subsequently...
We report the engineering of poly(ethylene glycol) (PEG) hydrogel particles using a mesoporous silica (MS) templating method via tuning PEG molecular weight, particle size, and presence or absence template investigate cell association biodistribution these particles. An ex vivo assay based on human whole blood that is more sensitive relevant than traditional cell-line assays for predicting in circulation behavior introduced. The MS@PEG (template present) with granulocytes monocytes higher...
The transport and the delivery of drugs through nanocarriers is a great challenge pharmacology. Since production liposomes to reduce toxicity doxorubicin in patients, plethora nanomaterials have been produced characterized. Although it widely known that elementary properties influence their vivo kinetics, such interaction often poorly investigated many preclinical studies. present study aims evaluate actual effect size shape on biodistribution set gold nanoparticles (GNPs) after intravenous...
Understanding the interactions between drug carriers and cells is of importance to enhance delivery therapeutics. The release therapeutics into different intracellular environments, such as lysosomes or cell cytoplasm, will impact their pharmacological activity. Herein, we investigate fate layer-by-layer (LbL)-assembled, submicrometer-sized polymer hydrogel capsules in a human colon cancer derived line, LIM1899. cellular uptake disulfide-stabilized poly(methacrylic acid) (PMASH) by...
Nanoengineered particles that can facilitate drug formulation and passively target tumors have reached the clinic in recent years. These early successes driven a new wave of significant innovation generation advanced particles. Recent developments enabling technologies chemistries led to control over key particle properties, including surface functionality, size, shape, rigidity. Combining these advances with rapid discovery many disease-related characteristics now offers opportunities for...
Particle shape is emerging as a key design parameter for tailoring the interactions between particles and cells. Herein, we report preparation of rod-shaped layer-by-layer (LbL)-assembled polymer hydrogel capsules with tunable aspect ratios (ARs). By templating spherical rodlike silica particles, disulfide-stabilized poly(methacrylic acid) (PMA HCs) different ARs (from 1 to 4) are generated. The influence capsule AR on cellular internalization intracellular fate was quantitatively...
Abstract Metal–organic coordination materials are of widespread interest because the coupled benefits inorganic and organic building blocks. These can be assembled into hollow capsules with a range properties, which include selective permeability, enhanced mechanical/thermal stability, stimuli‐responsiveness. Previous studies have primarily focused on assembly aspects metal‐coordination capsules; however, engineering metal‐specific functionality for capsule design has not been explored. A...
Abstract The preparation of pH‐labile polymer‐drug particles via mesoporous silica‐templated assembly for anticancer drug delivery into cancer cells is reported. conjugate synthesized thiol‐maleimide click chemistry using thiolated poly(methacrylic acid) (PMA SH ) and a doxorubicin (Dox) derivative. Drug‐loaded polymer that are stable under physiological conditions obtained through infiltration the conjugates silica particles, followed by cross‐linking PMA chains, subsequent removal porous...
A systematic and quantitative study on the role of capsule stiffness in cellular processing was performed using hyaluronic acid capsules with tunable constructed<italic>via</italic>continuous assembly polymers.
Highly purified outer membrane vesicles (OMVs) of the periodontal pathogens, Porphyromonas gingivalis, Treponema denticola and Tannerella forsythia were produced using tangential flow ultrafiltration, ultracentrifugation Optiprep density gradient separation. Cryo-TEM light scattering showed OMVs to be single lipid-bilayers with modal diameters 75 158 nm. Enumeration by nanoparticle flow-cytometry at same stage late exponential culture indicated that P. gingivalis was most prolific OMV...