A5061568687- Nanoparticle-Based Drug Delivery
- Nanoparticles: synthesis and applications
- RNA Interference and Gene Delivery
- Graphene and Nanomaterials Applications
- Advanced biosensing and bioanalysis techniques
- Microplastics and Plastic Pollution
- Gold and Silver Nanoparticles Synthesis and Applications
- Lipid Membrane Structure and Behavior
- Polymer Surface Interaction Studies
- Extracellular vesicles in disease
- Cytokine Signaling Pathways and Interactions
- Characterization and Applications of Magnetic Nanoparticles
- Nanoplatforms for cancer theranostics
- Monoclonal and Polyclonal Antibodies Research
- Air Quality and Health Impacts
- CAR-T cell therapy research
- RNA and protein synthesis mechanisms
- Porphyrin and Phthalocyanine Chemistry
- Microfluidic and Bio-sensing Technologies
- Viral Infections and Immunology Research
- Immunotherapy and Immune Responses
- Boron Compounds in Chemistry
- Cell death mechanisms and regulation
- MicroRNA in disease regulation
- Barrier Structure and Function Studies
University of Groningen
2016-2025
University of Rome Tor Vergata
2023-2024
World Health Organization
2024
University College Dublin
2009-2020
Center for Interdisciplinary Studies of Molecular Interactions
2020
Rijksmuseum
2020
Target (United States)
2019
University of Warwick
2014
22q11 Ireland
2014
Istituto Superiore di Sanità
2004-2011
Nanoparticles enter cells through active processes, thanks to their capability of interacting with the cellular machinery. The protein layer (corona) that forms on surface once nanoparticles are in contact biological fluids, such as cell serum, mediates interactions situ. As a consequence this, here we show same nanomaterial can lead very different outcomes, when exposed presence or absence preformed corona. In particular, silica serum have stronger adhesion membrane and higher...
The interactions between nanosized particles and living systems are commonly mediated by what adsorbs to the nanoparticle in biological environment, its biomolecular corona, rather than pristine surface. Here, we characterize adhesion toward cell membrane of nanoparticles different material size study how this is modulated presence or absence a corona on results corroborated with adsorption simple model supported lipid bilayers using quartz crystal microbalance. We conclude that proteins...
Nanoplastic debris, resulted from runoff and weathering breakdown of macro- microplastics, represents an emerging concern for marine ecosystems. The aim the present study was to investigate disposition toxicity polystyrene nanoparticles (NPs) in early development sea urchin embryos (Paracentrotus lividus). NPs with two different surface charges where chosen, carboxylated (PS-COOH) amine (PS-NH2) polystyrene, latter being a less common variant, known induce cell death several vitro systems....
Graphing graphene: Because the naming of graphene-based materials (GBMs) has led to confusion and inconsistency, a classification approach is necessary. Three physical-chemical properties GBMs have been defined by GRAPHENE Flagship Project European Union for unequivocal these (see grid).
Nanotechnology is expected to play a vital role in the rapidly developing field of nanomedicine, creating innovative solutions and therapies for currently untreatable diseases, providing new tools various biomedical applications, such as drug delivery gene therapy. In order optimize efficacy nanoparticle (NP) cells, it necessary understand mechanisms by which NPs are internalized this will likely determine their ultimate sub-cellular fate localisation. Here we have used pharmacological...
Cerium dioxide nanoparticles (CeO2 NPs) are increasingly being used as a catalyst in the automotive industry. Consequently, increasing amounts of CeO2 NPs expected to enter environment where their fate and potential impacts unknown. In this paper we describe effects three different sizes (14, 20, 29 nm) aquatic toxicity tests. each standard test medium (pH 7.4) aggregated (mean aggregate size approximately 400 nm). Four organisms covering trophic levels were investigated, i.e., unicellular...
Abstract The mechanism(s) of nanoparticle–cell interactions are still not understood. At present there is little knowledge the relevant length‐ and timescales for nanoparticle intracellular entry localization within cells, or cell‐specificity uptake localisation. Here, effect particle size on in‐vitro model fluorescent carboxyl‐modified polystyrene nanoparticles investigated in various cell lines. A range micro‐ defined sizes (40 nm to 2 μm) incubated with a series types, including HeLa A549...
Nanosized objects, such as nanoparticles and other drug carriers used in nanomedicine, once contact with biological environments are modified by adsorption of biomolecules on their surface. The presence this corona strongly affects the following interactions at cell organism levels. It has been shown that proteins can be recognized receptors. However, it is not known whether composition acquired layer also affect mechanisms use to enter cells. This particular importance when considering same...
Biomolecules adsorbed on nanoparticles are known to confer a biological identity nanoparticles, mediating the interactions with cells and barriers. However, how these molecules presented particle surface in milieu remains unclear. The central aim of this study is identify key protein recognition motifs link them specific cell-receptor interactions. Here, we employed an immuno-mapping technique quantify epitope presentations two major proteins serum corona, low-density lipoprotein...
It has been well established that the early stages of nanoparticle–cell interactions are governed, at least in part, by layer proteins and other biomolecules adsorbed slowly exchanged with surrounding biological media (biomolecular corona). Subsequent to membrane interactions, nanoparticles typically internalized into cell trafficked along defined pathways such as, many cases, endolysosomal pathway. Indeed, if original corona is partially retained on nanoparticle surface, this may play an...
Abstract In terms of lipid nanoparticle (LNP) engineering, the relationship between particle composition, delivery efficacy, and composition biocoronas that form around LNPs, is poorly understood. To explore this we analyze naturally efficacious biocorona compositions using an unbiased screening workflow. First, LNPs are complexed with plasma samples, from individual lean or obese male rats, then functionally evaluated in vitro. Then, a fast, automated, miniaturized method retrieves intact...
A spatio-temporal mapping of the uptake silica (SiO(2)) nanoparticles different sizes by lung epithelial cells has been obtained. Based on high control nanoparticle dispersion in cell media and exposure, one obtains reproducible quantitative time-resolved data using a combination flow cytometry, fluorescence electron microscopies. We are thereby able to give rather detailed account journey SiO(2) from early events their final sub-cellular localization.
Genotoxicity of commercial colloidal and laboratory-synthesized silica nanoparticles was tested using the single cell gel electrophoresis or Comet assay. By a carefully developed protocol careful characterization nanoparticle dispersions, assays were performed on 3T3-L1 fibroblasts with 3, 6, 24 h incubations 4 40 μg/ml nanoparticles. No significant genotoxicity observed for under conditions described, results independently validated in two separate laboratories, showing that vitro toxicity...
Positively charged polymers and nanoparticles (NPs) can be toxic to cells in various systems. Using human astrocytoma cells, we have previously shown that 50 nm amine-modified polystyrene NPs damage mitochondria induce cell death by apoptosis. Here provide comprehensive details of the cellular events occurring after exposure a time-resolved manner. We demonstrate accumulation lysosomes plays central role observed death, leading swelling release cathepsins into cytosol, which ultimately...
Nanoparticles (NPs) typically accumulate in lysosomes. However, their impact on lysosomal function, as well autophagy, a degradative pathway, is still not known. We have previously reported the 1321N1 cell line that amine-modified polystyrene (NH 2 -PS) NPs induce apoptosis through damage initiated lysosomes leading ultimately to release of content cytosol, followed by apoptosis. Here, using combination biochemical and biological approaches, we characterized mouse embryonic fibroblast...