A5077558844- Enzyme Catalysis and Immobilization
- Microbial Metabolic Engineering and Bioproduction
- Electrochemical sensors and biosensors
- Protein purification and stability
- Enzyme Production and Characterization
- Analytical Chemistry and Chromatography
- Biofuel production and bioconversion
- Amino Acid Enzymes and Metabolism
- Chemical Synthesis and Analysis
- Monoclonal and Polyclonal Antibodies Research
- Carbohydrate Chemistry and Synthesis
- Glycosylation and Glycoproteins Research
- Biochemical and Molecular Research
- Click Chemistry and Applications
- Enzyme Structure and Function
- Pancreatic function and diabetes
- Microfluidic and Capillary Electrophoresis Applications
- Biotin and Related Studies
- Peptidase Inhibition and Analysis
- Protein Hydrolysis and Bioactive Peptides
- Microbial Metabolites in Food Biotechnology
- Innovative Microfluidic and Catalytic Techniques Innovation
- Advanced Biosensing Techniques and Applications
- Polyamine Metabolism and Applications
- Enzyme-mediated dye degradation
Instituto de Catálisis y Petroleoquímica
2016-2025
Universidad Autónoma de Madrid
2016-2025
Institute of Catalysis and Petrochemistry
1990-2024
Consejo Superior de Investigaciones Científicas
2013-2023
Marie Curie
2015-2022
ORCID
2022
Instituto de Investigación en Ciencias de la Alimentación
2020-2022
CIC biomaGUNE
2015
John Wiley & Sons (Germany)
2015
Unidades Centrales Científico-Técnicas
2015
A number of bacterial lipases can be immobilized in a rapid and strong fashion on octyl-agarose gels (e.g., from Candida antarctica, Pseudomonas fluorescens, Rhizomucor miehei, Humicola lanuginosa, Mucor javanicus, Rhizopus niveus). Adsorption rates absence ammonium sulfate are higher than its presence, opposite to the observation for typical hydrophobic adsorption proteins. At 10 mM phosphate, is fairly selective allowing enzyme purification associated with their reversible immobilization....
Different strategies for the preparation of efficient and robust immobilized biocatalysts are here reviewed. physico-chemical approaches discussed. i.- The stabilization enzyme by any kind immobilization on pre-existing porous supports. ii.- enzymes multipoint covalent attachment support surfaces. iii.- Additional immobilized-stabilized physical or chemical modification with polymers. These three can be easily developed when in In addition to that, these derivatives optimal develop reaction...
Multifunctional supports containing epoxy groups are here proposed as a second generation of activated for covalent immobilization enzymes following the chemistry on any type support (hydrophobic or hydrophilic ones) under very mild experimental conditions (e.g., low ionic strength, neutral pH values, and temperatures). These multifunctional have been easily prepared by modifying small fraction (10-20%) contained in commercial supports. In this way, additional that were able to physically...
Abstract Sepabeads‐EP (a new epoxy support) has been utilized to immobilize‐stabilize the enzyme penicillin G acylase (PGA) via multipoint covalent attachment. These supports are very robust and suitable for industrial purposes. Also, internal geometry of support is composed by cylindrical pores surrounded convex surfaces (this offers a good geometrical congruence reaction with enzyme), it high superficial density groups (around 100 μmol/mL). features should permit intense enzyme‐support...
The properties of a new commercially available amino-epoxy support (amino-epoxy-Sepabeads) for immobilizing enzymes have been compared to those conventional epoxy supports. has layer groups over ethylenediamine that is covalently bound the support. Thus, this great anionic exchanger power and high number groups. We found advantages heterofunctional Immobilization proceeds at low ionic strength using amino Sepabeads while requiring monofunctional much more rapid supports than employing...
Three microbial lipases (those from Candida rugosa, Humicola lanuginosa, and Mucor miehei) have been found to exhibit a tendency form bimolecular aggregates in solution even at very low enzyme concentrations (44 microg/mL) the absence of detergent, as detected by gel filtration. The monomolecular enzymes was unique only concentration presence detergents. However, case lipase B antarctica, no could be identified high 1.2 mg/mL detergent. It has stated that structures display different...
The bacterial thermoalkalophilic lipases that hydrolyze saturated fatty acids at 60-75 degrees C and pH 8-10 are grouped as the lipase family I.5. We report here crystal structure of from Geobacillus thermocatenulatus, first a member I.5 showing an open configuration. Unexpectedly, enzyme activation involves large structural rearrangements around 70 amino concerted movement two lids, alpha6- alpha7-helices, unmasking active site. Central in restructuring process lids both transfer bulky...
Several strategies for linking antibodies (Abs) through their Fc region in an oriented manner have been proposed at the present time. By using these strategies, Fab of Ab is available antigen molecular recognition, leading to a more efficient interaction. Most are complex processes optimized mainly functionalization surfaces or microbeads. These methodologies imply though modification several steps purification use expensive immobilized proteins. Besides, magnetic nanoparticles (MNPs) turned...
Abstract In bio‐redox cascade reactions that are immobilized on porous supports, mass‐transfer limitations may impede the effective concentration of cofactor around corresponding dehydrogenases. This main drawback has been addressed by co‐immobilization both and recycling Herein, we report tailor‐made procedures to assemble three different orthogonal cascades in vitro (two selective reductions one oxidation) with situ cofactor‐regeneration. However, itself does not guarantee success...
Residual oil from babassu (Orbignya sp.), a low-cost raw material, was used in the enzymatic esterification for biodiesel production, using lipase B Candida antarctica (Novozym® 435) and ethanol. For first time literature, residual Novozym® 435 are being investigated to obtain biodiesel. In this communication, response surface methodology (RSM) central composite design (CCD) were optimize study effects of four factors (molar ratio (1:1–1:16, free fatty acids (FFAs) /alcohol), temperature...