A5003289620- Enzyme Catalysis and Immobilization
- Microbial Metabolic Engineering and Bioproduction
- Analytical Chemistry and Chromatography
- Electrochemical sensors and biosensors
- Biochemical and Molecular Research
- Protein Hydrolysis and Bioactive Peptides
- Innovative Microfluidic and Catalytic Techniques Innovation
- Pancreatic function and diabetes
- Proteins in Food Systems
- Microbial Metabolites in Food Biotechnology
- Enzyme Structure and Function
- Metabolism and Genetic Disorders
- 3D Printing in Biomedical Research
- Chemical Synthesis and Analysis
- Pharmacogenetics and Drug Metabolism
- Biotin and Related Studies
- Antibiotics Pharmacokinetics and Efficacy
- Phytase and its Applications
- Enzyme Production and Characterization
- Amino Acid Enzymes and Metabolism
- Protein Interaction Studies and Fluorescence Analysis
- Viral Infectious Diseases and Gene Expression in Insects
- Click Chemistry and Applications
Pontificia Universidad Católica de Valparaíso
2003-2023
Instituto de Catálisis y Petroleoquímica
2006-2010
Universidad Autónoma de Madrid
2006-2009
Consejo Superior de Investigaciones Científicas
2006-2009
Lipases from Candida rugosa (CRL) and lipase isoforms A B antarctica (CAL-A CAL-B) were adsorbed on aminated supports in the presence of detergents to have individual molecules. Then, one fraction was washed eliminate detergent, both preparations treated with glutaraldehyde. The detergent during cross-linking lipases support permitted an increase recovered activity (in some instances, even by a 10-fold factor). This higher than that exhibited just lipases, suggesting it not result protective...
Abstract The addition of a very small concentration detergent (in many instances under the critical micellar (cmc)) has been found to greatly increase activity immobilized lipases, using those from Pseudomonas fluorescens (PFL) and Candida antarctica (isoform B) as model enzymes. However, detergents may also have negative effect on enzyme activity; in fact, for all preparations substrates activity/detergent curve reached maximum value started decrease, even initial value. nature (SDS, CTAB,...
Cascade reactions have been described as efficient and universal tools, are of substantial interest in synthetic organic chemistry. This review article provides an overview the novel recent achievements enzyme cascade processes catalyzed by multi-enzymatic or chemoenzymatic systems. The examples here selected collect advances related to application sequential use enzymes natural genetically modified combination; second, important combination metal complex systems, finally we biocatalytic...
Desymmetrization of prochiral compounds is one the most efficient methods to obtain enantiomerically pure (yield close 100%); proof this its growing application over last decade in synthesis key chiral intermediates used preparation important biologically active compounds. Enzymes are highly specific, enantio- and regioselective catalysts that operate under relatively mild conditions therefore represent an interesting alternative chemical methods. This review describes significant progress,...
The effects of the reaction medium and substrate concentration were studied on selectivity Novozym 435 using asymmetric hydrolysis dimethyl-3-phenylglutarate as a model reaction. Results show that use choline chloride ChCl:urea/phosphate buffer 50% (v/v) increased by 16% (e.e = 88%) with respect to one in 100% phosphate 76%). Best results obtained when high concentrations (well above solubility limit, 27-fold) at pH 7 30 °C used. Under such conditions, R-monoester was produced an...
Defatted rapeseed meal (DRM) is a sub-valorized agro-industrial by-product, with high protein content whose peptides could have potential anticancer activity against cancer cell lines. The objective of the present study to obtain an enzymatic hydrolysate that inhibits proliferation on breast line (MCF-7), but not healthy human fibroblast cells. DRM was solubilized in alkaline medium extract (RAE). Acid precipitation proteins contained RAE recovered isolate (RPI). To produce hydrolysates, two...
Oligosaccharide and peptide extracts obtained separately from defatted rapeseed meal (DRM) have shown antiproliferative activities on the MCF-7 breast cancer cell line. However, oligosaccharide were not tested human fibroblasts low yields. The objective of present study was to combine two extracts, peptides oligosaccharides, that independently with commercial enzymes DRM, allowing improvement mass yield activity. DRM solubilized in an alkaline medium obtain insoluble residue (IMR) extract...
Different materials containing carboxylic groups have been functionalized with geranyl-amine molecules by using an EDC/NHS strategy. Chemical modification of the support was confirmed XRD, UV-spectrophotometer, and FT-IR. This geranyl-functionalized material successfully applied for four different strategies site-selective immobilization proteins at room temperature aqueous media. A reversible hydrophobic (lipases, phosphoglucosidases, or tyrosinase) performed in neutral pH yields from 40 to...