A5052800707- Pancreatic function and diabetes
- Signaling Pathways in Disease
- Genetics and Neurodevelopmental Disorders
- Zebrafish Biomedical Research Applications
- Diabetes and associated disorders
- Single-cell and spatial transcriptomics
- Immune Cell Function and Interaction
- Mitochondrial Function and Pathology
- RNA modifications and cancer
- Congenital heart defects research
- Birth, Development, and Health
- CRISPR and Genetic Engineering
- Effects and risks of endocrine disrupting chemicals
- MicroRNA in disease regulation
- Pancreatic and Hepatic Oncology Research
- Pancreatitis Pathology and Treatment
- Parasites and Host Interactions
- Diabetes Management and Research
- Liver Disease Diagnosis and Treatment
- Retinal Development and Disorders
- TGF-β signaling in diseases
- T-cell and B-cell Immunology
- IL-33, ST2, and ILC Pathways
- Regulation of Appetite and Obesity
- S100 Proteins and Annexins
University of Liège
2016-2025
Genomics (United Kingdom)
2020-2024
Zebra Biologics (United States)
2023-2024
The multiple vital functions of the human liver are performed by highly specialised parenchymal and non-parenchymal cells organised in complex collaborative sinusoidal units. Although crucial for homeostasis, cellular make-up remains to be fully elucidated. Here, single-cell RNA-sequencing was used unravel heterogeneity cells, particular hepatocytes (HEPs) hepatic stellate (HSCs).The transcriptome ~25,000 freshly isolated profiled using droplet-based RNA-sequencing. Recently published data...
Restoring damaged β-cells in diabetic patients by harnessing the plasticity of other pancreatic cells raises questions efficiency process and functionality new
Transfer RNA dynamics contribute to cancer development through regulation of codon-specific messenger translation. Specific aminoacyl-tRNA synthetases can either promote or suppress tumourigenesis. Here we show that valine synthetase (VARS) is a key player in the codon-biased translation reprogramming induced by resistance targeted (MAPK) therapy melanoma. The proteome rewiring patient-derived MAPK therapy-resistant melanoma biased towards usage and coincides with upregulation cognate tRNAs...
Defining the transcriptome and genetic pathways of pancreatic cells is great interest for elucidating molecular attributes pancreas disorders such as diabetes cancer. As function different cell types has been maintained during vertebrate evolution, comparison their transcriptomes across distant species a means to pinpoint genes under strong evolutionary constraints due crucial function, which have therefore preserved selective expression in these types. In this study, RNA-sequencing was...
Zebrafish are now widely used to study skeletal development and bone-related diseases. To that end, understanding osteoblast differentiation function, the expression of essential transcription factors, signaling molecules, extracellular matrix proteins is crucial. We isolated Sp7-expressing osteoblasts from 4-day-old larvae using a fluorescent reporter. identified two distinct subpopulations characterized their specific transcriptome as well structural, regulatory, profile. Based on...
Parasitic helminths induce the production of interleukin-4 (IL-4), which causes expansion virtual memory CD8+ T cells (TVM cells), a cell subset that contributes to control coinfection with intracellular pathogens. However, mechanisms regulating IL-4-dependent TVM activation and remain ill defined. Here, we used single-cell RNA sequencing identify pathways responses. Gene signature analysis identified cluster marked by CD22, canonical regulator B activation, as selective surface marker...
Abstract Cancer cells adapt their metabolism to support aberrant cell proliferation. However, the functional link between metabolic reprogramming and cycle progression remains largely unexplored. Mitochondria rely on transfer of multiple lipids from endoplasmic reticulum (ER) membranes be functional. Several mitochondrial‐derived metabolites influence cancer proliferation by modulating epigenome. Here, loss STARD7, a lipid protein whose expression is enhanced in breast cancer, shown lead...
Abstract Background Endocrine cells of the zebrafish digestive system play an important role in regulating metabolism and include pancreatic endocrine (PECs) clustered islets Langerhans enteroendocrine (EECs) scattered intestinal epithelium. Despite EECs PECs are being located distinct organs, their differentiation involves shared molecular mechanisms transcription factors. However, degree relatedness remains unexplored. In this study, we investigated comprehensively similarity by defining...
We report the generation and analysis of single-cell RNA-Seq data (> 38,000 cells) from mouse native retinae induced pluripotent stem cell (iPSC)-derived retinal organoids at four matched stages development spanning emergence major types. combine information temporal sampling, visualization 3D UMAP manifolds, pseudo-time RNA velocity analyses, to show that iPSC-derived broadly recapitulate developmental trajectories. However, we observe relaxation spatial transcriptome control, premature...
ARP/ASCL transcription factors are key determinants of cell fate specification in a wide variety tissues, coordinating the acquisition generic fates and specific subtype identities. How these factors, recognizing highly similar DNA motifs, display activities, is not yet fully understood. To address this issue, we overexpressed different zebrafish ascl1a-/- mutant embryos to determine which ones able rescue intestinal secretory lineage. We found that Ascl1a/b, Atoh1a/b Neurod1 all trigger...
Introduction Estrogenic endocrine disrupting chemicals (EDCs) such as diethylstilbestrol (DES) are known to alter the timing of puberty onset and reproductive function in females. Accumulating evidence suggests that steroid synthesis inhibitors ketoconazole (KTZ) or phthalates may also affect female health, however their mode action is poorly understood. Because hypothalamic activity very sensitive sex steroids, we aimed at determining whether how EDCs with different can transcriptome GnRH...
Non-alcoholic fatty liver disease (NAFLD) is the most common chronic hepatic pathology in Western countries. It encompasses a spectrum of conditions ranging from simple steatosis to more severe and progressive non-alcoholic steatohepatitis (NASH) that can lead hepatocellular carcinoma (HCC). Obesity related metabolic syndrome are important risk factors for development NAFLD, NASH HCC. DUSP3 small dual-specificity protein phosphatase with poorly known physiological function. We investigated...
Stimulation of pancreatic beta cell regeneration could be a therapeutic lead to treat diabetes. Unlike humans, the zebrafish can efficiently regenerate cells, notably from ductal progenitors. To gain insight into molecular pathways involved in this process, we established transcriptomic profile cells after ablation adult zebrafish. These data highlighted protein phosphatase calcineurin as new potential modulator regeneration. We showed that overexpression abolished regenerative response,...
<title>Abstract</title> Cancer cells adapt their metabolism to support aberrant cell proliferation. However, the functional link between metabolic reprogramming and cycle progression remains largely unexplored. Mitochondria rely on transfer of multiple lipids from endoplasmic reticulum (ER) membranes be functional. Several mitochondrial-derived metabolites influence cancer proliferation by modulating epigenome. Here we show that loss STARD7, a lipid protein whose expression is enhanced in...
Stimulation of pancreatic beta cell regeneration could be a therapeutic lead to treat diabetes. Unlike humans, the zebrafish can efficiently regenerate cells, notably from ductal progenitors. To gain insight into molecular pathways involved in this process, we established transcriptomic profile cells after ablation adult zebrafish. These data highlighted protein phosphatase calcineurin as new potential modulator regeneration. We showed that overexpression abolished regenerative response,...
Stimulation of pancreatic beta cell regeneration could be a therapeutic lead to treat diabetes. Unlike humans, the zebrafish can efficiently regenerate cells, notably from ductal progenitors. To gain insight into molecular pathways involved in this process, we established transcriptomic profile cells after ablation adult zebrafish. These data highlighted protein phosphatase calcineurin (CaN) as new potential modulator regeneration. We showed that CaN overexpression abolished regenerative...
Abstract We report the generation and analysis of single-cell RNA-Seq data (> 38,000 cells) from native iPSC-derived murine retina at four matched developmental stages spanning emergence major retinal cell types. combine information temporal sampling, visualization 3D UMAP manifolds, pseudo-time RNA velocity analyses, to show that aggregates broadly recapitulate trajectories. However, we relaxation spatial transcriptome control, premature dominance photoreceptor precursor cells,...