The fabrication of conventional PDMS on glass lab-on-a-chip (LOC) devices, using templates printed with a commercial (2299 US$) micro-stereo lithography 3D printer, is demonstrated. Printed replace clean room and photolithographic resources deliver resolutions 50 μm, up to 10 μm in localized hindrances, whereas the are smooth enough allow direct transfer proper sealing substrates. accommodate multiple thicknesses, from several mm within same template, no additional processing cost or effort....

Chemosensing based on angle-resolved surface plasmon resonance is demonstrated intact cell phones using a disposable optical coupler and software to configure illumination acquisition. This operates different applied for classical affinity assays with commercial chips custom-made tests embedded calibration. Measured performance (2.14x10−6 refractive index units) comparable compact SPR systems. Detailed facts of importance specialist readers are published as ”Supporting Information”. Such...

Versatile prototyping of 3D printed lab-on-a-chip devices, supporting different forms sample delivery, transport, functionalization and readout, is demonstrated with a consumer grade printer, which centralizes all critical fabrication tasks.

Sandwich lateral flow immunoassays (LFIAs) are limited at high antigen concentrations by the hook effect, leading to a contradictory decrease in test line (T) intensity and false-negative results. The effect is mainly associated with loss of T, research focuses on minimizing this effect. Nevertheless, control (C) also affected higher analyte concentrations, undesirably influencing T/C ratio LFIA readers. main aim work identify understand these concentration effects order develop ubiquitous...

Seeing sense: The detection of detailed absorption and emission responses caused by amines, CO, NOx is possible using an inexpensive array porphyrins as sensors combined with a computer screen web camera. computer-screen image shows the in presence triethylamine. [Zn]=[Zn(tpp)], [Fe]=[Fe(tppCl)] (tpp=5,10,15,20-tetraphenylporphyrin), BD=2,3,17,18-tetraethyl-7,8,12,13-tetramethyl-a,c-biladiene dihydrobromide.

The design of a smartphone imaging surface plasmon resonance (Smart-iSPR) system integrated with an affordable 3D-printed microfluidic SPR chip fabricated via facile manufacturing approach could pave the way towards development miniaturized and iSPR biosensors for emerging point-of-use applications. Conventional smartphone-based systems using soft photolithography fabrication microfluidics chips are costly, labour-intensive required specially-equipped light-controlled environment, that is...

Visible absorption features suitable for color recognition and micro-plate reading of a standard bioassay are performed by the combination computer screen used as programmable light source web camera detector. The method provides in this way highly available platform 'home tests' or 'self-tests', where requirement is to monitor well defined assays use economical instrumentation advantageous.

Exploiting the ubiquity of cell phones for quantitative chemical sensing imposes strong demands on interfacing devices. They should be autonomous, disposable, and integrate all necessary calibration actuation elements. In addition, a single design couple universally to variety phones, operate in their default configuration. Here, we demonstrate such concept its implementation as glucose meter that integrates finger pumps, unidirectional valves, references, focusing optics disposable device...

Als Chemosensoren können Mobiltelefone dienen, die durch Aufsetzen eines wegwerfbaren optischen Kopplers und eine Software zur Konfigurierung von Beleuchtung Aufnahme für winkelaufgelöste Oberflächenplasmonresonanz-Messungen (SPR) umgerüstet wurden. Der Koppler funktioniert mit verschiedenen Mobiltelefonen kann klassische Affinitätsassays kommerziellen Chips selbstgemachte Tests verwendet werden.

The unibody lab-on-a-chip (ULOC) concept entails a fast and affordable micro-prototyping system built around single monolithic 3D printed element (unibody). A consumer-grade stereo lithography (SL) printer can configure ULOCs with different forms of sample delivery, transport, handling readout, while minimizing material costs fabrication time. ULOC centralizes all complex procedures replaces the need for clean room resources, delivering prototypes less than 1 US$, which be in 10 min ready...

Acute intoxication incidents due to neurotoxic organophosphate (OP) insecticides are occasionally reported, related either suicidal attempts or occupational exposure the misuse of protective equipment. Among them, chlorpyrifos is a compound great controversy, which still authorized and easily accessible in many countries around world. However, screen for its markers, instrumental methods commonly applied, cannot enable rapid monitoring at an early stage intoxication. Therefore, this study,...

Artificial olfactory systems have been studied for the last two decades mainly from point of view features neuron receptor fields. Other fundamental olfaction properties only episodically considered in artificial systems. As a result, current are mostly intended as instruments and poor benefit biologists who may need tools to model test models. Herewith, we show how simple experimental approach can be used account several phenomena observed olfaction. An epithelium is formed disordered...

In this work, we investigated the sensing performance of epitaxial graphene on Si-face 4H-SiC (EG/SiC) for liquid-phase detection heavy metals (e.g., Pb and Cd), showing fast stable response low limit. The platform proposed includes 3D-printed microfluidic devices, which incorporate all features required to connect execute lab-on-chip (LOC) functions. obtained results indicate that EG exhibits excellent activity towards Cd ions. Several concentrations Pb2+ solutions, ranging from 125 nM 500...

A wide variety of 3D printing technologies have been used for the fabrication lab-on-a-chip (LOC) devices in recent years. Despite large number studies having examined use microfluidic devices, effect method on their performance has received little attention. In this paper, a comparison is shown between unibody-LOC micro-mixers, particular type monolithic design printed LOCs, fabricated polyjet, stereolithography (SLA) and fused deposition modelling (FDM or FFF) platforms, paying attention...

Nanostructured molecular assemblies may provide additional sensing properties not found in other arrangements of the same basic constituents. Among three-dimensional structures, nanotubes are particularly appealing for applications as chemical sensors, because potential inclusion different guests inside cavity or induced modification skeletal interaction after analyte binding. Porphyrins a class compounds characterized by brilliant properties, appearing also non-ordered solid-state...

The nitrocellulose (NC) membrane based lateral flow immunoassay device (LFID) is one of the most important and widely used biosensor platforms for point-of-care (PoC) diagnostics. However, analytical performance LFID has limitations its optimization restricted to bioassay chemistry, porosity, choice biolabel system. These bottom neck technical issues resulted from fact that conventional design principle not evolved many years, which limited advanced applications. Here we introduce a new...

Sehsinn: Die Detektion genauer Absorptions- und Emissionsantworten von Aminen, CO NOx gelingt mit einer einfachen Anordnung Porphyrinen als Sensoren, einem Computerbildschirm Web-Kamera. Im Bild ist die Situation in Gegenwart Triethylamin gezeigt. [Zn]=[Zn(tpp)], [Fe]=[Fe(tppCl)] (tpp=5,10,15,20-Tetraphenylporphyrin), BD=2,3,17,18-Tetraethyl-7,8,12,13-tetramethyl-a,c-biladien-dihydrobromid.

Securing food safety standards is crucial to protect the population from health-threatening contaminants. In case of pesticide residues, reference procedures typically find less than 1% tested samples being contaminated, thus indicating necessity for new tools able support smart and affordable prescreening. Here, we introduce a hybrid paper–lab-on-a-chip platform, which integrates on-demand injectors perform multiple step protocols in single disposable device. Simultaneous detection...