A5057382760- Enzyme Structure and Function
- Ion Transport and Channel Regulation
- Plant nutrient uptake and metabolism
- Ion channel regulation and function
- Plant Molecular Biology Research
- ATP Synthase and ATPases Research
- Reproductive System and Pregnancy
- Bacterial Genetics and Biotechnology
- Protein Structure and Dynamics
- Plant Reproductive Biology
- Lysosomal Storage Disorders Research
- Biochemical and Molecular Research
- Lipid Membrane Structure and Behavior
- Metabolism, Diabetes, and Cancer
- Photosynthetic Processes and Mechanisms
- RNA and protein synthesis mechanisms
- Plant tissue culture and regeneration
- Education, Healthcare and Sociology Research
- Assisted Reproductive Technology and Twin Pregnancy
- Membrane-based Ion Separation Techniques
- Glycosylation and Glycoproteins Research
- Reproductive Health and Technologies
- Inorganic Fluorides and Related Compounds
- Amino Acid Enzymes and Metabolism
- Cancer, Hypoxia, and Metabolism
Aarhus University
2015-2024
Danish National Research Foundation
2007-2017
University of California, San Francisco
2011-2016
Heinz Maier-Leibnitz Zentrum
2013
Aalborg University Hospital
1994-2011
Institute for Research in Biomedicine
2011
Centre for Biomedical Network Research on Rare Diseases
2011
University of Oslo
2011
Protein Express (United States)
2011
National Research Foundation
2010
Model building into experimental maps is a key element of structural biology, but can be both time consuming and error prone for low-resolution maps. Here we present Namdinator, an easy-to-use tool that enables the user to run molecular dynamics flexible fitting simulation followed by real-space refinement in automated manner through pipeline system. Namdinator will modify atomic model fit within cryo-EM or crystallography density maps, used advantageously initial models, geometrical...
Significance This paper reports the first structure of WT-human glucose transporter 1 (hGLUT1), to our knowledge, cocrystallized with inhibitors. The structures provide a template develop therapeutic inhibitors applicable cancers, because cancer cells become dependent on greatly increased consumption. dependence results in up-regulation expression, especially hGLUT1. bound include natural compound cytochalasin B and two series previously undescribed organic compounds that bind submicromolar...
Auxins are hormones that have central roles and control nearly all aspects of growth development in plants1–3. The proteins the PIN-FORMED (PIN) family (also known as auxin efflux carrier family) key participants this process export from cytosol to extracellular space4–9. Owing a lack structural biochemical data, molecular mechanism PIN-mediated transport is not understood. Here we present biophysical analysis together with three structures Arabidopsis thaliana PIN8: two outward-facing...
Plants are dependent on controlled sugar uptake for correct organ development and storage, apoplastic depletion is a defense strategy against microbial infections like rust mildew. Uptake of glucose other monosaccharides mediated by Sugar Transport Proteins, proton-coupled symporters from the Monosaccharide Transporter (MST) superfamily. We present 2.4 Å structure Arabidopsis thaliana high affinity transport protein, STP10, with bound. The explains recognition suggests proton donor/acceptor...
Abstract Sucrose import from photosynthetic tissues into the phloem is mediated by transporters low-affinity sucrose transporter family (SUC/SUT family). Furthermore, redistribution to other driven sap movement, product of high turgor pressure created this activity. Additionally, sink organs such as fruits, cereals and seeds that accumulate concentrations sugar also depend on active transport sucrose. Here we present structure sucrose–proton symporter, Arabidopsis thaliana SUC1, in an...
BACKGROUND: Previous trials of intravenous immunoglobulin (IvIg) treatment women with recurrent miscarriage (RM) have provided diverging results. This may be due to different inclusion criteria and suboptimal protocols in some trials. METHODS: According a computer-generated list, 58 at least four unexplained miscarriages were randomly assigned receive infusions high doses IvIg or placebo starting as soon the pregnancy test was positive. RESULTS: In intention-to-treat analysis, 45% live birth...
To obtain the initial crystallization hit and subsequent optimization remain major bottlenecks in membrane protein structure determination. Here we describe an approach to achieve diffraction quality crystals high concentrations of lipid detergent (HILIDE). Initial hits are achieved through a controlled variation protein/lipid/detergent ratios, optimized established by systematic screening with secondary detergents at that adapted for method. The is generally applicable native heterologously...
The human glucose transporters GLUT1 and GLUT3 have a central role in uptake as canonical members of the Sugar Porter (SP) family. share fully conserved substrate-binding site with identical substrate coordination, but differ significantly transport affinity line their physiological function. Here, we present 2.4 Å crystal structure an inward open conformation compare it using both structural functional data. Our work shows that interactions between cytosolic “SP motif” “A stabilize outward...
DsbB is an E. coli membrane protein that oxidizes DsbA, the primary disulfide donor present in periplasm.To understand how bonds are generated and introduced into secreted proteins, we determined crystal structure of a complex with DsbA endogenous ubiquinone at 3.7 Å resolution.The first revealed contains four-helix bundle scaffold transmembrane region one short membrane-parallel α-helix long periplasmic loop.Strikingly, disulfide-generating reaction center composed Cys41, Cys44, Arg48...
Previously, siblings of patients with idiopathic recurrent miscarriage (IRM) have been shown to a higher risk miscarriage. This study comprises two parts: (i) an epidemiological part, in which we introduce data on the frequency among 268 244 IRM and (ii) genetic part presenting from genome-wide linkage 38 affected sibling pairs IRM. All (probands) had experienced three or more miscarriages miscarriages. The were genotyped by Affymetrix GeneChip 50K XbaI platform non-parametric analysis was...