A5075938126- Legume Nitrogen Fixing Symbiosis
- Plant Molecular Biology Research
- Genetic and Environmental Crop Studies
- Plant Genetic and Mutation Studies
- Plant Reproductive Biology
- Microtubule and mitosis dynamics
- Plant nutrient uptake and metabolism
- Plant Stress Responses and Tolerance
- Genetic Neurodegenerative Diseases
- Plant tissue culture and regeneration
- Plant Micronutrient Interactions and Effects
- Cellular transport and secretion
- Cardiomyopathy and Myosin Studies
- Phytase and its Applications
- Agricultural pest management studies
- Plant-Microbe Interactions and Immunity
- Wheat and Barley Genetics and Pathology
- Plant Gene Expression Analysis
- Muscle Physiology and Disorders
- Agronomic Practices and Intercropping Systems
- Soybean genetics and cultivation
- Gene expression and cancer classification
- Polysaccharides and Plant Cell Walls
- Plant Parasitism and Resistance
- Genetics and Plant Breeding
Agricultural Research Organization
2021-2024
National Institute of Plant Genome Research
2015-2024
Ministry of Agriculture and Rural Development
2023-2024
Cytokinin group of phytohormones regulate root elongation and branching during post-embryonic development. Cytokinin-degrading enzymes cytokinin oxidases/dehydrogenases (CKXs) have been deployed to investigate biological activities engineer growth. We expressed chickpea oxidase 6 (CaCKX6) under the control a root-specific promoter CaWRKY31 in Arabidopsis thaliana having determinate indeterminate growth patterns, respectively, study effect depletion on drought tolerance. Root-specific...
Myosins are important motor proteins that associate with the actin cytoskeleton. Structurally, myosins function as heteromeric complexes where smaller light chains, such calmodulin (CaM), bind to isoleucine-glutamine (IQ) domains in neck region facilitate mechano-enzymatic activity. We recently identified Arabidopsis CaM-like (CML) CML13 and CML14 interactors of containing multiple IQ domains, including a myosin VIII. Here, we demonstrate CaM, CML13, all four VIII isoforms. Among CMLs tested...
Flower and seed coat colour are important agronomic traits in chickpea (Cicer arietinum L.). Cultivated chickpeas of two types namely, desi (dark seeded, purple flowered) kabuli (light white flowered). There has been limited information about the molecular mechanism underlying variation flower coats chickpea. We profiled anthocyanin proanthocyanidin (PA) contents flowers coats. Tissue-specific silencing genes encoding a basic helix-loop-helix (CabHLH) protein tonoplast-localized multidrug...
Calcium signaling plays an important role in adaptation and developmental processes plants animals. A class of calcium sensors, known as Calcineurin B-like (CBL) proteins sense specific temporal changes cytosolic Ca(2+) concentration regulate activities a group ser/thr protein kinases called CBL-interacting (CIPKs). Although number CIPKs have been shown to play crucial roles the regulation stress signaling, no study on function CIPK25 or its orthologs has reported so far. In present study,...
In our recent publication, we have shown that a member of the Laccase family, LACCASE2 (LAC2) acted as negative regulator lignin deposition in root xylem tissue Arabidopsis thaliana. LAC2 messenger RNA (mRNA) level was post-transcriptionally regulated by microRNA 397b, which showed increased expression under water and phosphate deficiency, resulting downregulation this report, investigated growth an economically important legume crop chickpea (Cicer arietinum L.) response to natural drought...
Abstract Identifying potential molecular tags for drought tolerance is essential achieving higher crop productivity under stress. We employed an integrated genomics-assisted breeding and functional genomics strategy involving association mapping, fine map-based cloning, haplotyping transcript profiling in the introgression lines (ILs)- near isogenic (NILs)-based panel mapping population of chickpea (Cicer arietinum). This combinatorial approach delineated a bHLH (basic helix–loop–helix)...
Abstract Lateral roots are a major component of root system architecture, and lateral count (LRC) positively contributes to yield under drought in chickpea. To understand the genetic regulation LRC, biparental mapping population derived from two chickpea accessions having contrasting LRCs was genotyped by sequencing, phenotyped map four quantitative trait loci (QTLs) contributing 13–32% LRC variation. A single- nucleotide polymorphism tightly linked locus highest variation located on coding...
Chickpea (Cicer arietinum L.) is the third most important food legume crop. Seed size economically trait for chickpea. To understand genetic regulation of seed in chickpea, present study established a three-way association CT repeat length variation simple sequence (SSR) myo-inositol monophosphatase gene (CaIMP) with weight and phytic acid content by large scale validation genotyping set genetically diverse germplasm accessions two reciprocal intra-specific mapping populations. Germplasms...
Calcium ion (Ca2+) is a ubiquitous second messenger that transmits various internal and external signals including stresses and, therefore, important for plants' response process. Calcineurin B-like proteins (CBLs) are one of the plant calcium sensors, which sense convey changes in cytosolic Ca2+-concentration A search four leguminous (soybean, Medicago truncatula, common bean chickpea) genomes identified 9 to 15 genes each species encode CBL proteins. Sequence analyses peptides coding...
Abstract Clonal propagation of plants by induction adventitious roots (ARs) from stem cuttings is a requisite step in breeding programs. A major barrier exists for propagating valuable that naturally have low capacity to form ARs. Due the central role auxin organogenesis, indole-3-butyric acid (IBA) often used as part commercial rooting mixtures, yet many recalcitrant do not ARs response this treatment. Here, we describe synthesis and screening focused library synthetic conjugates Eucalyptus...
The distribution of myosin VIII ATM1 tail in association with the plasma membrane is often observed coordination that cortical microtubules (MTs). prevailing hypothesis between organization MTs and proteins results from inhibition free lateral diffusion by barriers formed MTs. Since positioning relatively stable, we ask: can it affect MTs? Myosin co-localized remorin 6.6, position which also stable. Overexpression led to a larger fraction lower rate orientation dispersion. In addition,...
Argania spinosa trees have attracted attention in recent years due to their high resistance extreme climate conditions. Initial domestication activities practiced Morocco. Here we report on selection and vegetative propagation of A. grown Israel. Trees yielding relatively amounts fruit were propagated by rooting stem cuttings. High variability ability was found among the 30 clones selected. In-depth comparison a difficult-to-root (ARS7) easy-to-root (ARS1) clone revealed that rooted cuttings...
Abstract Myosins are a crucial motor protein associated with the actin cytoskeleton in eukaryotic cells. Structurally, myosins form heteromeric complexes, smaller light chains such as calmodulin (CaM) bound to isoleucine–glutamine (IQ) domains neck region. These interactions facilitate mechano-enzymatic activity. Recently, we identified Arabidopsis CaM-like (CML) proteins CML13 and CML14 interactors containing multiple IQ domains, that function myosin VIII chains. This study demonstrates...
The endoplasmic reticulum (ER) is the largest organelle in terms of membrane content, occupying entire cytoplasmic volume. It tethered to cell cortex through ER-plasma contact sites (EPCS). Previous studies have shown that EPCSs labelled by VAP27 align with cortical microtubules, and ER tubules elongate along microtubules. Here, we addressed question whether this relationship bidirectional, influencing microtubule organisation. Using TIRF microscopy track dynamics simultaneously, demonstrate...
Abstract Myosins are important motor proteins that associate with the actin cytoskeleton. Structurally, myosins function as heteromeric complexes where smaller light chains, such calmodulin (CaM), bind to isoleucine-glutamine (IQ) domains in neck regions facilitate mechano-enzymatic activity. We recently identified Arabidopsis CaM-like (CML) proteins, CML13 and CML14 interactors of containing multiple IQ domains, including a member myosin VIII class. Here, using vivo vitro assays we...
Abstract Flower and seed coat colour are important agronomic traits in chickpea ( Cicer arietinum L.). Cultivated chickpeas of two types namely, desi (dark seeded, purple flower) kabuli (light white flower). There has been limited information about the molecular mechanism underlying variation flower coats chickpea. We profiled anthocyanin proanthocyanidin (PA) contents flowers coats. Tissue-specific silencing genes encoding a basic helix-loop-helix (CabHLH) protein tonoplast-localized...