A5016223683- Plant Molecular Biology Research
- CRISPR and Genetic Engineering
- Plant nutrient uptake and metabolism
- Chromosomal and Genetic Variations
- Legume Nitrogen Fixing Symbiosis
- Genetic and Environmental Crop Studies
- Agronomic Practices and Intercropping Systems
- GABA and Rice Research
- Plant Stress Responses and Tolerance
- Plant Virus Research Studies
- Plant and Biological Electrophysiology Studies
National Institute of Plant Genome Research
2020-2024
Cytokinin group of phytohormones regulate root elongation and branching during post-embryonic development. Cytokinin-degrading enzymes cytokinin oxidases/dehydrogenases (CKXs) have been deployed to investigate biological activities engineer growth. We expressed chickpea oxidase 6 (CaCKX6) under the control a root-specific promoter CaWRKY31 in Arabidopsis thaliana having determinate indeterminate growth patterns, respectively, study effect depletion on drought tolerance. Root-specific...
Abstract Chickpea is considered recalcitrant to in vitro tissue culture. The Clustered, Regularly Interspaced Short Palindromic Repeats/CRISPR-associated protein 9 (CRISPR/Cas9) based genome editing chickpea can remove the bottleneck of limited genetic variation this cash crop rich nutrients and protein. However, generation stable mutant lines using CRISPR/Cas9 requires efficient highly reproducible transformation approaches. We modified a binary vector pPZP200 by introducing codon-optimized...