A5033042329- Biofuel production and bioconversion
- Enzyme Production and Characterization
- Microbial Metabolites in Food Biotechnology
- Enzyme Catalysis and Immobilization
- Microbial Metabolic Engineering and Bioproduction
- Carbohydrate Chemistry and Synthesis
- Glycosylation and Glycoproteins Research
- Food composition and properties
- Polysaccharides and Plant Cell Walls
- Seaweed-derived Bioactive Compounds
- Probiotics and Fermented Foods
- Algal biology and biofuel production
- Protein Hydrolysis and Bioactive Peptides
- Phytochemicals and Antioxidant Activities
- Pancreatic function and diabetes
- Bacteriophages and microbial interactions
- Advanced Cellulose Research Studies
- Phytase and its Applications
- Marine and coastal plant biology
- Natural Antidiabetic Agents Studies
- Catalysis for Biomass Conversion
- Studies on Chitinases and Chitosanases
- Enzyme Structure and Function
- Gut microbiota and health
- Genomics and Phylogenetic Studies
Lund University
2016-2025
Technical University of Denmark
2020
Karolinska University Hospital
2009
Karolinska Institutet
1974-2009
Wake Forest University
2009
Getinge (Sweden)
2009
Malmö University
1983-2008
Swedish Defence Research Agency
2005
University of Bath
2002-2003
Newcastle University
2002
Abstract In today's world, there is an increasing trend towards the use of renewable, cheap and readily available biomass in production a wide variety fine bulk chemicals different biorefineries. Biorefineries utilize activities microbial cells their enzymes to convert into target products. Many these processes require which are operationally stable at high temperature thus allowing e.g . easy mixing, better substrate solubility, mass transfer rate, lowered risk contamination. Thermophiles...
The mucolytic human gut microbiota specialist Akkermansia muciniphila is proposed to boost mucin-secretion by the host, thereby being a key player in mucus turnover. Mucin glycan utilization requires removal of protective caps, notably fucose and sialic acid, but enzymatic details this process remain largely unknown. Here, we describe specificities ten A. glycoside hydrolases, which collectively remove all known sialyl fucosyl mucin caps including those on double-sulfated epitopes....
The thermophile Rhodothermus marinus produces extracellular polysaccharides (EPSs) that forms a distinct cellular capsule. Here, the first data on EPS production in strains DSM4252T and MAT493 are reported compared. Cultures of both strains, supplemented with either glucose, sucrose, lactose or maltose showed were produced exponential stationary growth phase was boosted by supplementation, while lactose. latter higher, resulting 8.8 (DSM4252T) 13.7 mg EPS/g cell dry weight (MAT493) cultures...
To compare xylans from forestry with agricultural origins, hardwood xylan (birch) and cereal arabinoxylan (rye) were hydrolyzed using two variants of the xylanase RmXyn10A, full-length enzyme catalytic module only, Rhodothermus marinus. Cultivations four selected bacterial species, xylooligosaccharide (XOS) containing hydrolysates as carbon source, showed selective growth Lactobacillus brevis DSMZ 1264 Bifidobacterium adolescentis ATCC 15703. Both strains confirmed to utilize XOS fraction...
Effects of xylooligosaccharides (XOSs) as well a mixture XOS, inulin, oligofructose, and partially hydrolyzed guar gum (MIX) in mice fed high-fat diet (HFD) were studied. Control groups an HFD or low-fat diet. Special attention was paid to the cecal composition gut microbiota formation short-chain fatty acids, but metabolic parameters also documented. The XOS group had significantly higher cecum levels acetic, propionic, butyric acids than group, acid content MIX group. contained more...
Onion waste is a renewable raw material, rich in different molecular species of the antioxidant quercetin. To utilize this resource, an environmentally sustainable procedure has been developed, using pressurized hot water to extract quercetin species, followed by biocatalytic conversion glycosides and carbohydrates. Two recombinantly expressed thermostable β-glucosidases, Thermotoga neapolitana β-glucosidase A B, were utilized as catalysts. These enzymes maintain activity at temperatures...
Galectins are defined by a conserved β-galactoside binding site that has been linked to many of their important functions in e.g. cell adhesion, signaling, and intracellular trafficking. Weak adjacent sites may enhance or decrease affinity for natural β-galactoside-containing glycoconjugates, but little is known about the biological role this modulation (fine specificity). We have now produced 10 mutants human galectin-3, with changes these altered carbohydrate-binding fine specificity...
Xylan was extracted from wheat bran after heat pretreatment in water using either an autoclave or a microwave oven. Xylooligosaccharides (XOS) were produced the xylan thermostable xylanase RmXyn10A and potential prebiotic properties of XOS studied vitro with different human gut bacteria: Lactobacillus brevis (DSMZ 1269), Bifidobacterium adolescentis (ATCC 15703) two strains recently isolated lactic acid bacteria species pair Weissella cibaria/confusa. The highest yield (arabino)xylan...
This paper presents a trans-disciplinary assessment of new and innovative biorefinery concepts producing high-value chemical compounds from residues agriculture, food forest industries. There is significant potential biomass in Sweden suitable for the extraction various compounds, including upgrading by biocatalytic processes, addition to current energy generation. Two examples presented are quercetin extracted onion waste pressurised hot water conjunction with enzymatic hydrolysis, betulin...
World quinoa production is increasing due its high nutritional value. As a consequence, large quantities of stalks accumulate as unused byproducts. Here, we verify the presence saponins in and present biorefinery approach with feedstock, using an integrated processing scheme to separate saponins, xylan cellulose. Saponins were extracted pressurized hot water extraction (PHWE), optimized by central composite experimental design (rotatable 22) temperature time factors. Xylan was from residual...
The success of establishing bioeconomies replacing current economies based on fossil resources largely depends our ability to degrade recalcitrant lignocellulosic biomass. This study explores the potential employing various enzymes acting synergistically previously pretreated agricultural side streams (corn bran, oat hull, soluble and insoluble bran). Degrees synergy (oligosaccharide yield obtained with enzyme combination divided by sum yields individual enzymes) up 88 were obtained....
Sugarcane processing roughly generates 54 million tonnes sugarcane bagasse (SCB)/year, making SCB an important material for upgrading to value-added molecules. In this study, integrated scheme was developed separating xylan, lignin and cellulose, followed by production of xylo-oligosaccharides (XOS) from SCB. Xylan extraction conditions were screened in: (1) single extractions in NaOH (0.25, 0.5, or 1 M), 121 °C (1 bar), 30 60 min; (2) 3 × repeated cycles 2 min (3) pressurized liquid (PLE),...
Production of fish meal and plant-based feed proteins continues to increase meet the growing demand for seafood, leading impacts on marine terrestrial ecosystems. Microbial such as single-cell (SCPs) have been introduced alternatives since they can replace current ingredients, e.g., soybean, which are associated with negative environmental impacts. protein production also enables utilization grain processing side-streams feedstock sources. This study assesses yeast-based SCP using oat...