A5045822931- Tuberculosis Research and Epidemiology
- Mycobacterium research and diagnosis
- Bacterial Genetics and Biotechnology
- Bacterial Infections and Vaccines
- RNA and protein synthesis mechanisms
- Genomics and Phylogenetic Studies
- Antibiotic Resistance in Bacteria
- DNA Repair Mechanisms
- Bacteriophages and microbial interactions
- Mitochondrial Function and Pathology
- Microbial infections and disease research
- Synthesis and biological activity
- Diagnosis and treatment of tuberculosis
- Pneumonia and Respiratory Infections
- Cancer therapeutics and mechanisms
- Infectious Diseases and Tuberculosis
- Infective Endocarditis Diagnosis and Management
- Streptococcal Infections and Treatments
- Protist diversity and phylogeny
- Biochemical and Structural Characterization
- Escherichia coli research studies
- Phenothiazines and Benzothiazines Synthesis and Activities
- Inflammatory Bowel Disease
- Evolution and Genetic Dynamics
- Quinazolinone synthesis and applications
University of Oslo
2016-2025
Oslo University Hospital
2016-2025
Coalition for Epidemic Preparedness Innovations
2021
International Centre of Insect Physiology and Ecology
2019
Med-Storm Innovation (Norway)
2009
Czech Academy of Sciences, Institute of Microbiology
1998-2007
University of Michigan
1998
Abstract Mismatch repair (MMR) is a near ubiquitous pathway, essential for the maintenance of genome stability. Members MutS and MutL protein families perform key steps in mismatch correction. Despite major importance this MutS–MutL are absent almost all Actinobacteria many Archaea. However, these organisms exhibit rates spectra spontaneous mutations similar to MMR-bearing species, suggesting existence an alternative canonical MutS–MutL-based MMR. Here we report that Mycobacterium smegmatis...
Alzheimer disease (AD) is a devastating neurological associated with progressive loss of mental skills and cognitive physical functions whose etiology not completely understood. Here, our goal was to simultaneously uncover novel known molecular targets in the structured layers hippocampus olfactory bulbs that may contribute early hippocampal synaptic deficits dysfunction AD mice. Spatially resolved transcriptomics used identify high-confidence genes were differentially regulated mice...
ABSTRACT Although Mycobacterium ulcerans , M. marinum and haemophilum are closely related, their exact taxonomic placements have not been determined. We performed gas chromatography of fatty acids alcohols, as well DNA-DNA hybridization 16S rRNA gene sequence analysis, to clarify relationships each other tuberculosis. were most related one another, displayed very strong genetic affinities tuberculosis ; they actually the two mycobacterial species outside complex . was more distinct from it...
Summary Expression of type IV pili appears to be a requisite determinant infectivity for the strict human pathogens Neisseria gonorrhoeae and meningitidis. The assembly these colonization factors is complex process. This report describes new pilus‐assembly gene, pilG , that immediately precedes gonococcal ( Gc) pilD gene encoding pre‐pilin leader peptidase. nucleotide sequence this region revealed single complete open reading frame whose derived polypeptide displayed significant identities...
Mycobacterium tuberculosis complex species display relatively static genomes and 99.9% nucleotide sequence identity. Studying the evolutionary history of such monomorphic bacteria is a difficult challenging task.We found that single-nucleotide polymorphism (SNP) analysis DNA repair, recombination replication (3R) genes in comprehensive selection M. strains from across world, yielded surprisingly high levels polymorphisms as compared to house-keeping genes, making it possible distinguish...
The bacterial pathogen Neisseria meningitidis expresses long, thin, retractile fibers (called type IV pili) from its cell surface and uses these adhesive structures to mediate primary attachment epithelial cells during host colonization invasion. PilQ is an outer membrane protein complex that essential for the translocation of pili across membrane. Here, we present structure determined by cryoelectron microscopy 12 Å resolution. dominant feature a large central cavity, formed four arm...
In all sexual organisms, adaptations exist that secure the safe reassortment of homologous alleles and prevent intrusion potentially hazardous alien DNA. Some bacteria engage in a simple form sex known as transformation. human pathogen Neisseria meningitidis related bacterial species, transformation by exogenous DNA is regulated presence specific Uptake Sequence (DUS), which present thousands copies respective genomes. DUS affects limiting uptake recombination favour The mechanisms...
ABSTRACT Recent genotyping studies of Mycobacterium tuberculosis in Ethiopia have reported the identification a new phylogenetically distinct M. lineage, lineage 7. We therefore investigated genetic diversity and association specific lineages with sociodemographic clinical parameters among pulmonary TB patients Amhara Region, Ethiopia. DNA was isolated from tuberculosis- positive sputum specimens ( n = 240) analyzed by PCR 24-locus mycobacterial interspersed repetitive unit–variable-number...
Type IV pili are polymeric fibers which protrude from the cell surface and play a critical role in adhesion invasion by pathogenic bacteria. The secretion of across periplasm outer membrane is mediated specialized secretin protein, PilQ, but way this large channel formed unknown. Using NMR, we derived structures periplasmic domains N. meningitidis PilQ: N-terminus shown to consist two β-domains, unique type pilus-dependent secretins. structure second β-domain revealed an eight-stranded...
The aim of this study was to investigate the relationship between aquaporin (AQP) water channel expression and pathological features early untreated inflammatory bowel disease (IBD) in humans.Patients suspected have IBD on basis predefined symptoms, including abdominal pain, diarrhea, and/or blood stool for more than 10 days, were examined at local hospital. Colonoscopy with biopsies performed samples taken. Patients who did not meet diagnostic criteria displayed no evidence infection or...
ABSTRACT PilQ is a member of the secretin family outer membrane proteins and specifically involved in secretion type IV pili Neisseria meningitidis, gonorrhoeae , Pseudomonas aeruginosa . The quaternary structure from N. meningitidis was analyzed by transmission electron microscopy using negative stain. Single particle averaging carried out with total data set 650 individual particles, which produced projection map generated 296 particles at an estimated resolution 2.6 nm. Oligomeric adopts...
The frequently occurring DNA uptake sequence (DUS), recognized as a 10-bp repeat, is required for efficient genetic transformation in the human pathogens Neisseria meningitidis and gonorrhoeae. Genome scanning DUS occurrences three different species of demonstrated that 76% nearly 2,000 neisserial were found to have two semiconserved base pairs extending from 5' end constitute 12-mer repeat. Plasmids containing sequential variants tested their ability transform N. gonorrhoeae, was outperform...
Abstract Background Efficient natural transformation in Neisseria requires the presence of short DNA uptake sequences (DUSs). Doubts remain whether DUSs propagate by pure selfish molecular drive or are selected for 'safe sex' among conspecifics. Results Six neisserial genomes were aligned to identify gene conversion fragments, DUS distribution, spacing, and conservation. We found a strong link between recombination DUS: spacing matches size fragments; with shorter fragments have more...
Neisseria meningitidis can be the causative agent of meningitis or septicemia. This bacterium expresses type IV pili, which mediate a variety functions, including autoagglutination, twitching motility, biofilm formation, adherence, and DNA uptake during transformation. The secretin PilQ supports pilus extrusion retraction, but it also requires auxiliary proteins for its assembly localization in outer membrane. Here we have studied physical properties lipoprotein PilP examined interaction...
As an intracellular human pathogen, Mycobacterium tuberculosis (Mtb) is facing multiple stressful stimuli inside the macrophage and granuloma. Understanding Mtb responses to stress essential identify new virulence factors pathways that play a role in survival of tubercle bacillus. The main goal this study was map regulatory networks differentially expressed (DE) transcripts upon various forms genotoxic stress. We exposed cells oxidative (H2O2 or paraquat), nitrosative (DETA/NO), alkylation...
PurposeTo identify essential oils (EOs) active against non-growing stationary phase Mycobacterium abscessus and multidrug-resistant M. strains.MethodsThe activity of EOs both log was evaluated by colony forming unit (CFU) assay minimum inhibitory concentration (MIC) testing.ResultsWe assessed the 80 found 12 (Cinnamon, Satureja montana, Palmarosa, Lemon eucalyptus, Honey myrtle, Combava, Health shield, Mandarin, Thyme, Rosewood, Valerian Root Basil) at 0.5% to be growing abscessus. Among...
Repeated sequence signatures are characteristic features of all genomic DNA. We have made a rigorous search for repeat sequences in the human pathogens Neisseriameningitidis, Neisseria gonorrhoeae and Haemophilus influenzae found that by far most frequent 9–10mers residing within coding regions DNA uptake (DUS) required natural genetic transformation. More importantly, we significantly higher density DUS genes involved repair, recombination, restriction‐modification replication than any...