A5002063674- Tuberculosis Research and Epidemiology
- Mycobacterium research and diagnosis
- Infectious Diseases and Tuberculosis
- Diagnosis and treatment of tuberculosis
- RNA and protein synthesis mechanisms
- Genomics and Phylogenetic Studies
- Pneumonia and Respiratory Infections
- CRISPR and Genetic Engineering
- Pneumocystis jirovecii pneumonia detection and treatment
- Advanced biosensing and bioanalysis techniques
- Bacteriophages and microbial interactions
- vaccines and immunoinformatics approaches
- Antibiotic Resistance in Bacteria
- Infectious Diseases and Mycology
- Forensic Anthropology and Bioarchaeology Studies
- Vibrio bacteria research studies
- Chronic Lymphocytic Leukemia Research
- Paleopathology and ancient diseases
- Innovative Microfluidic and Catalytic Techniques Innovation
- Biosensors and Analytical Detection
- Electrochemical sensors and biosensors
- Image Processing Techniques and Applications
- Salmonella and Campylobacter epidemiology
- Zoonotic diseases and public health
- Yersinia bacterium, plague, ectoparasites research
Nord University
2025
Inserm
2021-2025
Université Paris-Saclay
2015-2024
Université Paris Cité
2011-2024
Infection, Anti-microbiens, Modélisation, Evolution
2020-2024
Université Bourgogne Franche-Comté
2024
London School of Hygiene & Tropical Medicine
2024
Institut Pasteur de Lille
2024
Center for Infection and Immunity of Lille
2024
AgroParisTech
2024
Molecular typing based on 12 loci containing variable numbers of tandem repeats mycobacterial interspersed repetitive units (MIRU-VNTRs) has been adopted in combination with spoligotyping as the basis for large-scale, high-throughput genotyping Mycobacterium tuberculosis. However, even these two methods is still less discriminatory than IS6110 fingerprinting. Here, we define an optimized set MIRU-VNTR a significantly higher power. The resolution and stability/robustness 29 were analyzed,...
Abstract Background The Direct Repeat locus of the Mycobacterium tuberculosis complex (MTC) is a member CRISPR (Clustered regularly interspaced short palindromic repeats) sequences family. Spoligotyping widely used PCR-based reverse-hybridization blotting technique that assays genetic diversity this and useful both for clinical laboratory, molecular epidemiology, evolutionary population genetics. It easy, robust, cheap, produces highly diverse portable numerical results, as result...
ABSTRACT We analyzed a global collection of Mycobacterium tuberculosis strains using 212 single nucleotide polymorphism (SNP) markers. SNP diversity was high (average across all SNPs, 0.19), and 96% the locus pairs were in complete linkage disequilibrium. Cluster analyses identified six deeply branching, phylogenetically distinct cluster groups (SCGs) five subgroups. The SCGs strongly associated with geographical origin M. samples birthplace human hosts. most ancestral (SCG-1) predominated...
Laboratory surveillance systems for salmonellosis should ideally be based on the rapid serotyping and subtyping of isolates. However, current typing methods are limited in both speed precision. Using 783 strains isolates belonging to 130 serotypes, we show here that a new family DNA repeats named CRISPR (clustered regularly interspaced short palindromic repeats) is highly polymorphic Salmonella. We found polymorphism was strongly correlated with serotype multilocus sequence type....
Abstract Analysis of genome sequencing data from >100,000 genomes Mycobacterium tuberculosis complex using TB-Annotator software revealed a previously unknown lineage, proposed name L10, in central Africa. Phylogenetic reconstruction suggests L10 could represent missing link the evolutionary and geographic migration histories M. africanum.
ABSTRACT Bone and soft tissue samples from 85 ancient Egyptian mummies were analyzed for the presence of Mycobacterium tuberculosis complex DNA (aDNA) further characterized by spoligotyping. The specimens obtained individuals different tomb complexes in Thebes West, Upper Egypt, which used upper social class burials between Middle Kingdom (since ca. 2050 BC) Late Period (until 500 BC). A total 25 provided a specific positive signal amplification 123-bp fragment repetitive element IS 6110 ,...
ABSTRACT The present update on the global distribution of Mycobacterium tuberculosis complex spoligotypes provides both octal and binary descriptions for M. complex, including bovis , from >90 countries (13,008 patterns grouped into 813 shared types containing 11,708 isolates 1,300 orphan patterns). A number potential indices were developed to summarize information biogeographical specificity a given type, as well its geographical spreading (matching code index, respectively). To...
Abstract We present a short summary of recent observations on the global distribution major clades Mycobacterium tuberculosis complex, causative agent tuberculosis. This was defined by data-mining an international spoligotyping database, SpolDB3. database contains 11,708 patterns from as many clinical isolates originating more than 90 countries. The spoligotypes were clustered into 813 shared types. A total 1,300 orphan (clinical showing unique spoligotype) also detected.
Two-step electrochemical patterning methods have been employed to elaborate composite nanomaterials formed with multiwalled carbon nanotubes (MWCNTs) coated polypyrrole (PPy) and redox PAMAM dendrimers. The nanomaterial has demonstrated as a molecular transducer for DNA detection. nanocomposite MWCNTs-PPy by wrapping the PPy film on MWCNTs during polymerization of pyrrole gold electrode. layer was modified dendrimers fourth generation (PAMAM G4) covalent bonding electro-oxidation method....
Herein we present a microfluidic-multiplexed platform that integrates electrochemical sensors based on carbon nanotubes associated with ferrocene as redox marker (carbon nanotube (CNT)/ferrocene) for direct detection of pathogenic viral DNA from Hepatitis C and genomic Mycobacterium tuberculosis in clinical isolates. By operating the fluidic device under high flow (150 μl/min), formation very thin depletion layer at sensor surface (δS = 230 nm) enhances capture rate up to one strand per...
ABSTRACT We analyzed DNA polymorphisms in 455 Mycobacterium tuberculosis complex isolates from patients to evaluate the biodiversity of tubercle bacilli Ouest province, Cameroon. The phenotypic and genotypic identification methods gave concordant results for 99.5% M. (413 strains) 90% africanum (41 strains). bovis was isolated only one patient. Analysis regions difference (RD4, RD9, RD10) proved be an accurate rapid method distinguishing between unusual members complex. Whereas strains were...
We give an update on the worldwide spoligotype database, which now contains 3,319 patterns of Mycobacterium tuberculosis in 47 countries, with 259 shared types, i.e., identical spoligotypes by two or more patient isolates. The types contained a total 2,779 (84%) all Seven major genetic groups represented 37% clustered Two (119 and 137) were found almost exclusively USA accounted for 9% remaining 1,517 isolates scattered into 252 different spoligotypes. This database constitutes tool pattern...
Mycobacterium tuberculosis complex species display relatively static genomes and 99.9% nucleotide sequence identity. Studying the evolutionary history of such monomorphic bacteria is a difficult challenging task.We found that single-nucleotide polymorphism (SNP) analysis DNA repair, recombination replication (3R) genes in comprehensive selection M. strains from across world, yielded surprisingly high levels polymorphisms as compared to house-keeping genes, making it possible distinguish...
The aims of the present study were to implement a microbead-based 'spoligotyping' technique and evaluate improvements by addition panel 25 extra spacers that we expected provide an increased resolution on principal genetic group 1 (PGG 1) strains. We confirmed high sensitivity reproducibility classical using 43 spacer obtained perfect agreement between membrane-based techniques. further demonstrated increase in discriminative power extended 68 format for differentiation PGG clinical...
Evolution of the direct repeat region in Mycobacterium tuberculosis has created unique spoligotype signatures specifically associated with IS6110-defined strain families. Spoligotyping may enable analysis population structure different settings and will rapid identification families that acquire drug resistance or escape protective immunity vaccine trials.
Abstract One hundred five Mycobacterium tuberculosis clinical isolates from the Delhi area were typed by spoligotyping; 45 patterns identified. Comparison with an international spoligotype database showed type 26, (22%), 54 (12%), and 1, Beijing (8%), as most common. Eighteen spoligotypes did not match any existing pattern.
Abstract We give an update on the worldwide spoligotype database, which now contains 3,319 patterns of Mycobacterium tuberculosis in 47 countries, with 259 shared types, i.e., identical spoligotypes by two or more patient isolates. The types contained a total 2,779 (84%) all Seven major genetic groups represented 37% clustered Two (119 and 137) were found almost exclusively USA accounted for 9% remaining 1,517 isolates scattered into 252 different spoligotypes. This database constitutes tool...