A5063054836- Tuberculosis Research and Epidemiology
- Mycobacterium research and diagnosis
- Antibiotic Resistance in Bacteria
- Antibiotic Use and Resistance
- Diagnosis and treatment of tuberculosis
- Infectious Diseases and Tuberculosis
- Bacterial Identification and Susceptibility Testing
- Pneumocystis jirovecii pneumonia detection and treatment
- Clostridium difficile and Clostridium perfringens research
- Antimicrobial Resistance in Staphylococcus
- Antibiotics Pharmacokinetics and Efficacy
- Bacteriophages and microbial interactions
- Pneumonia and Respiratory Infections
- Pharmaceutical and Antibiotic Environmental Impacts
- Streptococcal Infections and Treatments
- Salmonella and Campylobacter epidemiology
- Fungal Infections and Studies
- Vibrio bacteria research studies
- Antifungal resistance and susceptibility
- Viral gastroenteritis research and epidemiology
- Microscopic Colitis
- Biosimilars and Bioanalytical Methods
- Immunodeficiency and Autoimmune Disorders
- Infectious Diseases and Mycology
- Cytomegalovirus and herpesvirus research
Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán
2016-2025
NIMS University
2013
Universidad de Guanajuato
2008
National Institute of Nutrition
2008
Central Arkansas Veterans Healthcare System
2006
Rutgers New Jersey Medical School
2006
Victorian Infectious Diseases Reference Laboratory
2006
Michigan State University
2006
Howard Hughes Medical Institute
2003
Albert Einstein College of Medicine
2003
ABSTRACT We analyzed a global collection of Mycobacterium tuberculosis strains using 212 single nucleotide polymorphism (SNP) markers. SNP diversity was high (average across all SNPs, 0.19), and 96% the locus pairs were in complete linkage disequilibrium. Cluster analyses identified six deeply branching, phylogenetically distinct cluster groups (SCGs) five subgroups. The SCGs strongly associated with geographical origin M. samples birthplace human hosts. most ancestral (SCG-1) predominated...
The molecular basis for isoniazid resistance in Mycobacterium tuberculosis is complex. Putative mutations have been identified katG, ahpC, inhA, kasA, and ndh. However, small sample sizes related potential biases selection precluded the development of statistically valid significant population genetic analyses clinical resistance. We present first large-scale analysis 240 alleles previously associated with a diverse set 608 isoniazid-susceptible 403 isoniazid-resistant M. isolates. detected...
To determine the clinical consequences of pulmonary tuberculosis (TB) among patients with diabetes mellitus (DM).We conducted a prospective study TB in Southern Mexico. From 1995 to 2010, acid-fast bacilli or Mycobacterium sputum samples underwent epidemiological, and microbiological evaluation. Annual follow-ups were performed ascertain treatment outcome, recurrence, relapse reinfection.The prevalence DM 1262 was 29.63% (n=374). Patients had more severe manifestations (cavities any size on...
OBJECTIVE—To determine the impact of diabetes on rates tuberculosis in a region where both diseases are prevalent. RESEARCH DESIGN AND METHODS—Data from population-based cohort patients with pulmonary undergoing clinical and mycobacteriologic evaluation (isolation, identification, drug-susceptibility testing, IS6110-based genotyping spoligotyping) were linked to 2000 National Health Survey (ENSA2000), national probabilistic, polystage, stratified, cluster household survey civilian,...
<b>Background:</b> In most low income countries there are twice as many cases of tuberculosis (TB) reported among men than women, a difference commonly attributed to biological and epidemiological characteristics well socioeconomic cultural barriers in access health care. The World Health Organization has encouraged gender specific comparisons TB rates determine whether women with less likely be diagnosed, reported, treated. A study was undertaken identify based differences patients...
Mutations at position 306 of embB (embB306) have been proposed as a marker for ethambutol resistance in Mycobacterium tuberculosis; however, recent reports embB306 mutations ethambutol-susceptible isolates caused us to question the biological role this mutation. We tested 1,020 clinical M. tuberculosis with different drug susceptibility patterns and geographical origins associations between mutations, patterns, major genetic group. One hundred (10%) contained mutation embB306; only 55 these...
Interleukin 12Rβ1 (IL-12Rβ1)-deficient patients are prone to clinical disease caused by mycobacteria, Salmonella, and other intramacrophagic pathogens, probably because of impaired interleukin 12-dependent interferon γ production. About 25% also display mucocutaneous candidiasis, owing 23-dependent 17 immunity. The features outcome candidiasis in these have not been described before, our knowledge. We report here the signs 35 with IL-12Rβ1 deficiency.Most (n = 71) 76 episodes were...
Carbapenem-resistant Enterobacteriaceae (CRE) infections have emerged as a serious threat to health worldwide. They are associated with increased morbidity and mortality capable of silently colonizing the gastrointestinal tract. Because this, there is great interest characterize epidemiology CRE carriage acquisition in healthcare facilities. The aim this study was determine prevalence factors fecal (CRE-fc), risk for incident cases.A cohort conducted at tertiary care hospital from January...
Background Human tuberculosis caused by M. bovis is a zoonosis presently considered sporadic in developed countries, but remains poorly studied problem low and middle resource countries. The disease humans mainly attributed to unpasteurized dairy products consumption. However, transmission due exposure of infected animals has been also recognized. prevalence infection associated risk factors have insufficiently characterized among farm workers (DFW) exposed settings with poor control bovine...
Human tuberculosis caused by Mycobacterium bovis is believed to be frequent in developing countries. Transmission usually through ingestion of unpasteurized dairy products, although airborne contagion possible. Disease M. or clinically indistinguishable from each other. The aim this study was determine the factors associated with disease. Retrospective analysis all culture-positive cases and 2000 2015, a Mexican tertiary-care centre. Sociodemographic, clinical, radiographic data medical...
Objective: To describe empirical antimicrobial prescription on admission in patients with severe COVID-19, the prevalence of Hospital-Acquired Infections, and susceptibility patterns causing organisms. Methods: In this prospective cohort study a tertiary care center Mexico City, we included consecutive admitted COVID-19 between March 20th June 10th evaluated occurrence HAI. Results: 794 were during period. Empiric antibiotic treatment was started 92% (731/794); most frequent regimes...
Background: worldwide, the frequency of tuberculosis among older people almost triples that observed young adults. Objective: to describe clinical and epidemiological consequences pulmonary people. Methods: we screened persons with a cough lasting more than 2 weeks in Southern Mexico from March 1995 February 2007. We collected mycobacteriological information (isolation, identification, drug-susceptibility testing IS6110-based genotyping spoligotyping) individuals bacteriologically confirmed...
Carbapenem-resistant Enterobacteriaceae carrying New Delhi metallo-β-lactamase 1 (NDM-1) have rarely been reported in Latin America. We report of an outbreak caused by a blaNDM-1-harboring plasmid spread through different bacterial species, including Escherichia coli (ST617) and Enterobacter cloacae (ST182) isolates from the same patient three Klebsiella pneumoniae (ST22) derived epidemiologically related patients. IncFII plasmids were found all strains. Measures to control applied successfully.
This report presents phenotypic and genetic data on the prevalence characteristics of extended-spectrum β-lactamases (ESBLs) representative carbapenemases-producing Gram-negative species in Mexico.A total 52 centers participated, 43 hospital-based laboratories 9 external laboratories. The distribution antimicrobial resistance for Escherichia coli, Klebsiella pneumoniae, Enterobacter cloacae complex, Acinetobacter baumannii Pseudomonas aeruginosa selected clinical specimens from January 1 to...
ABSTRACT The utility of luciferase reporter mycobacteriophages (LRPs) for detection, identification, and antibiotic susceptibility testing Mycobacterium tuberculosis was prospectively evaluated in a clinical microbiology laboratory Mexico City, Mexico. Five hundred twenty-three consecutive sputum samples submitted to the during 5-month period were included this study. These specimens cultivated Middlebrook 7H9 (MADC), MGIT, Löwenstein-Jensen (LJ) media. Of 71 mycobacterial isolates...
The use of polymerase chain reaction-single-stranded conformational polymorphism (PCR-SSCP) to study rpoB gene mutations in rifampin-resistant (RIFr) Mycobacterium tuberculosis has yielded contradictory results. To determine the sensitivity this method, we analyzed 35 RIFr strains and 11 rifampin-susceptible (RIFs) strains, using DNA sequencing core region for comparison. Of RIFr, 24 had a PCR-SSCP pattern identical that H37Rv; other four different patterns. RIFs patterns H37Rv. assay was...
The definitive diagnosis of spontaneous bacterial peritonitis is made by a positive ascitic fluid culture. Causative organisms cannot be isolated in up to 65% patients with well-defined peritonitis, probably due inadequate ascites culture techniques. We prospectively compared two methods: conventional (on chocolate agar and thioglycolate broth) modified (inoculation 10 ml tryptic soy broth blood bottle at the patient's bedside). In 10-month period, 31 cirrhotic met our diagnostic criteria...
Summary After encounter with Mycobacterium tuberculosis , a series of non‐uniform immune responses are triggered that define the course infection. Eight M. strains were selected from prospective population‐based study pulmonary patients (1995–2003) based on relevant clinical/epidemiological patterns and tested in well‐characterized BALB/c mouse model progressive tuberculosis. In addition, new transmissibility consisting prolonged cohousing (up to 60 days) infected naïve animals was tested....
Cronobacter spp. ( Enterobacter sakazakii ) includes gram-negative opportunistic foodborne pathogens known as rare but important causes of life-threatening neonatal infections. However, the pathogenic mechanism is not yet clear. In this study, 43 isolates Cronobacter, from human and nonhuman sources, were analyzed. A total four clusters identified 32 DNA pulsotypes observed by pulsed-field gel electrophoresis. addition, 86% able to adhere HEp-2 cells 35% invasive, being most efficient....
Background Mycobacterium tuberculosis causes the majority of (TB) cases in humans; however, developing countries, human TB caused by M. bovis may be frequent but undetected. Human is considered a zoonosis; transmission mainly through consumption unpasteurized dairy products, and it less frequently attributed to animal-to-human or human-to-human contact. We describe trends isolation from samples first-line drug susceptibility during 15-year period referral laboratory located tertiary care...
Tuberculosis remains a serious threat worldwide. For this reason, it is necessary to identify agents that shorten the duration of treatment, strengthen host immune system, and/or decrease damage caused by infection. Statins are drugs reduce plasma cholesterol levels and have immunomodulatory, anti-inflammatory antimicrobial effects. Although there evidence statins may contribute containment
Vaccination with Mycobacterium bovis bacille Calmette-Guérin (BCG) has variable efficacy in preventing tuberculosis. We hypothesized that some of this variation might be due to differences among BCG strains. To test this, neonates Orizaba, Mexico, were vaccinated one three different strains (BCG-Brazil [BBCG], BCG-Denmark [DBCG], or BCG-Japan [JBCG]). One year after vaccination, peripheral blood mononuclear cells (PBMC) obtained and recall immune responses culture filtrate proteins (CFP)...
ObjectivesTo study the prevalence, risk factors, outcome, and molecular epidemiology in patients with bacteremia caused by extended-spectrum β-lactamase (ESBL)-producing Klebsiella pneumoniae (Kp) (cases), comparison a susceptible Kp (controls).MethodsThis was retrospective case–control including all episodes of for period 1993 to 2002 at referral hospital adults Mexico. ESBL production tested E-test. All isolates were typed pulsed field gel electrophoresis (PFGE). A subset underwent plasmid...