A5011864259- Mycobacterium research and diagnosis
- Reproductive tract infections research
- Tuberculosis Research and Epidemiology
- Infectious Diseases and Mycology
- Genomics and Phylogenetic Studies
- Antibiotic Resistance in Bacteria
- Plant Pathogens and Fungal Diseases
- Urinary Tract Infections Management
- Hepatitis B Virus Studies
- Bacteriophages and microbial interactions
- Bacterial Identification and Susceptibility Testing
- Microbial infections and disease research
- Liver Disease Diagnosis and Treatment
- Streptococcal Infections and Treatments
- Syphilis Diagnosis and Treatment
- Antifungal resistance and susceptibility
- Hepatitis C virus research
- Bacterial Infections and Vaccines
- Pneumonia and Respiratory Infections
- Viral gastroenteritis research and epidemiology
- Ginseng Biological Effects and Applications
- Cervical Cancer and HPV Research
- Mycorrhizal Fungi and Plant Interactions
- Respiratory viral infections research
- Adolescent Sexual and Reproductive Health
Nigerian Defence Academy
2025
Universiti Tunku Abdul Rahman
2015-2024
Creative Commons
2023
University of Malaya
2005-2016
University Malaya Medical Centre
1997-2016
Universiti Malaysia Kelantan
2015
Chinese University of Hong Kong
2011
University of Hong Kong
2011
Monash University Malaysia
2009
National Institutes of Biotechnology Malaysia
2008
Abstract Background The Direct Repeat locus of the Mycobacterium tuberculosis complex (MTC) is a member CRISPR (Clustered regularly interspaced short palindromic repeats) sequences family. Spoligotyping widely used PCR-based reverse-hybridization blotting technique that assays genetic diversity this and useful both for clinical laboratory, molecular epidemiology, evolutionary population genetics. It easy, robust, cheap, produces highly diverse portable numerical results, as result...
Summary Mycobacterium abscessus is a rapidly growing non-tuberculous mycobacterial species that has been associated with wide spectrum of human infections. As the classification and biology this organism still not well understood, comparative genomic analysis on members may provide further insights their taxonomy, phylogeny, pathogenicity other information contribute to better management The MabsBase described in paper user-friendly database providing access whole-genome sequences newly...
A total of 61 isolates Salmonella enteritidis were analyzed by the techniques pulsed-field gel electrophoresis (PFGE) and ribotyping. Twenty-three from Zurich, Switzerland, 38 University Hospital, Kuala Lumpur, Malaysia. Five Malaysian hospital-related outbreak strains shown to be indistinguishable PFGE analysis following digestion with three different restriction endonucleases, XbaI (5'-TCTAGA-3'), SpeI (5'-ACTAGT-3'), AvrII (5'-CCTAGG-3'). The pattern an isolate a suspected carrier staff...
Mycobacterium abscessus (Ma) is an emerging human pathogen that causes both soft tissue infections and systemic disease. We present the first comparative whole-genome study of Ma strains isolated from patients wide geographical origin. found a high proportion accessory strain-specific genes indicating open, non-conservative pan-genome structure, clear evidence rapid phage-mediated evolution. Although we fewer virulence factors in compared to M. tuberculosis, our data indicated evolves...
In mycobacteria, conjugation differs from the canonical Hfr model, but is still poorly understood. Here, we quantified this evolutionary processe in a natural mycobacterial population, taking advantage of large clinical strain collection emerging pathogen Mycobacterium abscessus (MAB).Multilocus sequence typing confirmed existence three M. subspecies, and unravelled extensive allelic exchange between them. Furthermore, an asymmetrical gene flow occurring these main lineages was detected,...
Dematiaceous fungi (black fungi) are a heterogeneous group of present in diverse environments worldwide. Many species this known to cause allergic reactions and potentially fatal diseases humans animals, especially tropical subtropical climates. This study represents the first survey dematiaceous Malaysia provides observations on their diversity as well vitro response antifungal drugs. Seventy-five strains isolated from various clinical specimens were identified by morphology an internal...
Antimicrobial activities against reference Gram-positive (Staphylococcus aureus, Enterococcus faecalis) and Gram-negative (Escherichia coli, Pseudomonas aeruginosa) bacteria Candida albicans were tested on 191 plant extracts obtained from more than 30 families of plants found in the state Sabah, Malaysia. The by a disk-diffusion technique which antimicrobial activity was evaluated based ability to diffuse through agar affect target organisms. Callicarpa erioclona Schau. (Verbenaceae),...
Background:In many parts of Asia, the inaccessibility and high cost diagnostic tests have hampered study community-acquired pneumonia (CAP) caused by atypical respiratory pathogens.Objective:This surveillance examined frequency infection with Mycoplasma pneumoniae, Chlamydophila Legionella pneumophila in 1756 patients presenting signs symptoms CAP at 12 medical centres using standardised laboratory techniques interpretation criteria all participating centres.Methods:Diagnosis current was...
A variable-number tandem-repeat (VNTR) typing assay for the differentiation of Mycobacterium abscessus strains was developed. This showed complete reproducibility, locus stability, and a discriminatory power (Hunter-Gaston index [HGDI] 0.9563) that is superior to multilocus sequencing. It promising tool investigation epidemiology nosocomial outbreaks.
Abstract It is an urgent need to develop new drugs for Mycobacterium tuberculosis (Mtb) and the enzyme, dihydrofolate reductase (DHFR) a recognised drug target. The crystal structures of methotrexate binding mt - h -DHFR separately indicate that glycerol (GOL) site likely be critical function selective inhibitors. We have used in silico methods screen NCI small molecule database group related compounds were obtained inhibit activity showed bactericidal effects against test Mtb strain. poses...
A cross-sectional study was conducted from 10 January to 9 April 2012, determine the seroprevalence of tuberculosis (TB) all captive Asian elephants and their handlers in six locations Peninsular Malaysia. In addition, trunk-wash samples were examined for tubercle bacillus by culture polymerase chain reaction (PCR). For 63 149 elephant handlers, TB estimated at 20.4% 24.8%, respectively. From 151 trunkwash samples, 24 acid-fast isolates obtained, 23 which identified hsp65-based sequencing as...
Liver is the vital organ of metabolism and excretion. It also an important target toxicity xenobiotics oxidative stress. The study was carried out to determine expression profile caspase-3 gene in carbon tetrachloride (CCl4) induced hepatotoxicity wistar rats. Thiry-five (35) rats were divided into seven groups five each group given varying treatments along with intraperitonial administration CCl4. effects aqueous methanolic leaf extracts Azadirachta indica determined by evaluating liver...
Hepatitis B virus (HBV) has been divided into 10 genotypes, A to J, based on an 8% nucleotide sequence divergence between genotypes. The conventional practice of using a single set primers amplify near-complete HBV genome is hampered by its low analytical sensitivity. current overlapping conserved primer sets complete in clinical sample limited the lack pan-primers detect all In this study, we designed six highly conserved, cover genome. We our design sequences 5,154 genomes genotypes I...
Cladosporium sphaerospermum is one of the most widely distributed allergens causing serious problems in patients with respiratory tract disease. We report 26,644,473-bp draft genome sequence and gene annotation C. UM843. Analysis led to finding genes associated sphaerospermum's melanin biosynthesis, allergens, antifungal drug resistance.
Abstract Background Mycobacterium abscessus is a rapidly growing mycobacterium that often associated with human infections. The taxonomy of this species has undergone several revisions and still being debated. In study, we sequenced the genomes 12 M. strains used phylogenomic analysis to perform subspecies classification. Results A data mining approach was rank select informative genes based on relative entropy metric for construction phylogenetic tree. resulting tree topology similar...
Daldinia eschscholtzii is a wood-inhabiting fungus that causes wood decay under certain conditions. It has broad host range and produces large repertoire of potentially bioactive compounds. However, there no extensive genome analysis on this fungal species. Two isolates (UM 1400 UM 1020) from human specimens were identified as by morphological features ITS-based phylogenetic analysis. Both genomes similar in size with 10,822 predicted genes (35.8 Mb) 11,120 1020 (35.5 Mb). A total 751 gene...
The outbreak of extensively drug-resistant tuberculosis (XDR-TB) has become an increasing problem in many TB-burdened countries. underlying drug resistance mechanisms, including the genetic variation favored by selective pressure resistant population, are partially understood. Recently, first case XDR-TB was reported Malaysia. However, detailed genotype family and mechanisms formation multiple drugs unknown. We sequenced whole genome UM 1072388579 strain with a 2-kb insert-size library...
Abstract Cladosporium sphaerospermum , a dematiaceous saprophytic fungus commonly found in diverse environments, has been reported to cause allergy and other occasional diseases humans. However, its basic biology genetic information are largely unexplored. A clinical isolate C. genome, UM 843, was re-sequenced combined with previously generated sequences form model 26.89 Mb genome containing 9,652 predicted genes. Functional annotation on genes suggests the ability of this degrade...