A5059677833- Bacillus and Francisella bacterial research
- Tuberculosis Research and Epidemiology
- Mycobacterium research and diagnosis
- Bacteriophages and microbial interactions
- Bacterial Genetics and Biotechnology
- Monoclonal and Polyclonal Antibodies Research
- Diagnosis and treatment of tuberculosis
- Botulinum Toxin and Related Neurological Disorders
- Microbial Inactivation Methods
- Escherichia coli research studies
- Viral gastroenteritis research and epidemiology
- Antibiotic Resistance in Bacteria
- RNA and protein synthesis mechanisms
- Pneumonia and Respiratory Infections
- Biosensors and Analytical Detection
- Protein purification and stability
- Infectious Diseases and Tuberculosis
- Probiotics and Fermented Foods
- Neurological disorders and treatments
- Yersinia bacterium, plague, ectoparasites research
- SARS-CoV-2 and COVID-19 Research
- COVID-19 Clinical Research Studies
- Advanced biosensing and bioanalysis techniques
- SARS-CoV-2 detection and testing
- Bacterial biofilms and quorum sensing
State Research Center for Applied Microbiology and Biotechnology
2015-2024
Federal Service for Surveillance on Consumer Rights Protection and Human Wellbeing
2008-2023
Ministry of Health of the Russian Federation
2021
Centers for Disease Control and Prevention
2015
Arkhangel'skiy Klinicheskiy Onkologicheskiy Dispanser
2015
Northern State Medical University
2015
Institute of Bioorganic Chemistry
2008
Research Institute of Medical Genetics of Russian Academy of Medical Sciences
2001
Abstract Background The Direct Repeat locus of the Mycobacterium tuberculosis complex (MTC) is a member CRISPR (Clustered regularly interspaced short palindromic repeats) sequences family. Spoligotyping widely used PCR-based reverse-hybridization blotting technique that assays genetic diversity this and useful both for clinical laboratory, molecular epidemiology, evolutionary population genetics. It easy, robust, cheap, produces highly diverse portable numerical results, as result...
Ever decreasing efficiency of antibiotic treatment due to growing resistance pathogenic bacteria is a critical issue in clinical practice. The two generally accepted major approaches this problem are the search for new antibiotics and development adjuvants enhance antimicrobial activity known compounds. It was therefore aim present study test whether alkylresorcinols, class phenolic lipids, can be used as potentiate effect various classes antibiotics. Alkylresorcinols were combined with 12...
The genotypic characteristics and drug susceptibility profiles of clinical isolates Mycobacterium tuberculosis recovered from prison hospital patients in the Tula region (central Russia) during 2001 2002 are reported. emergence multi-drug-resistant (TB) poses a major health risk to population, with economic implications for TB control. Prisons serve as continuous source transmission. results showed that members LAM Beijing families contributors epidemiological picture population studied. two...
Pollution of the environment by crude oil and products (represented various types compounds, mainly aliphatic, mono- polyaromatic hydrocarbons) poses a global problem. The strain Pseudomonas veronii 7-41 can grow on medium-chain n-alkanes (C8-C12) polycyclic aromatic hydrocarbons such as naphthalene. We performed genetic analysis physiological/biochemical characterization cultivated in mineral medium with decane, naphthalene or mixture hydrocarbons. genes responsible for degradation alkanes...
Acquired resistance to antituberculosis drugs decreases effective treatment options and the likelihood of success. We identified risk factors for acquisition drug during multidrug-resistant tuberculosis (MDR TB) evaluated effect on outcomes. Data were collected prospectively from adults Arkhangelsk Oblast, Russia, who had pulmonary MDR TB 2005-2008. Acquisition capreomycin extensively drug-resistant more likely among patients received <3 than >3 (9.4% vs. 0% 8.6% 0.8%, respectively). Poor...
Anthrax and botulism are dangerous infectious diseases that can be fatal unless detected treated quickly. Fatalities from these primarily due to endopeptidase toxins secreted by the pathogens. Rapid sensitive detection of presence active is key element for protection natural outbreaks anthrax botulism, as well threat bioterrorism. We describe an ultrasensitive polymerase chain reaction (PCR)-based assay detecting proteolytic activity botulinum using composite probes consisting covalent...
Lethal factor (LF), a zinc-dependent protease of high specificity produced by Bacillus anthracis, is the effector component binary toxin that causes death in anthrax. New therapeutics targeting are required to reduce systemic anthrax-related fatalities. In particular, new insights into LF catalytic mechanism will be useful for development inhibitors. We evaluated minimal length formation bona fide substrates using substrate phage display. Phage-based selection yielded cleaved seven times...
We analyzed IS6110-associated polymorphisms in the phospholipase C genes of 107 isolates Mycobacterium tuberculosis selected to be representative circulating central Russia. found that majority Latin American-Mediterranean family strains contained an insertion a unique position plcA gene, suggesting common ancestor. This can serve as specific genetic marker for this group, which we designate LAM-RUS family.
Anti-DNA autoantibodies are responsible for tissue injury in lupus. A subset of DNA-specific antibodies capable DNA cleavage can be even more harmful after entering the living cells by destroying nuclear DNA. Origins anti-DNA not fully understood, and mechanism induction DNA-cleaving activity remains speculative. The autoantibody BV04-01 derived from lupus-prone mouse is only DNA-hydrolyzing immunoglobulin with known 3D structure. Identification analysis homologous to may help understand...
Botulism is one of the most serious food intoxications, manifesting as prolonged paralytic conditions. This disease usually result consumption poor quality canned or smoked foods, so inhabitants many countries world are exposed to risk this kind poisoning every year. In view severity poisonings caused by botulinum neurotoxins, monoclonal antibodies (mAbs) show great promise because their targeting action, lack allergic reactions and serum sickness. The use a cocktail mAbs increases...
Shiga toxin-producing Escherichia coli (STEC) food-borne infections are reported worldwide and represent a serious problem for public healthcare. In the Russian Federation there is little information on epidemiology etiology of STEC-infections as well molecular-genetic peculiarities STEC pathogens.Our aim was to describe outbreak hemorrhagic colitis (HC) along with hemolytic uremic syndrome (HUS), enterocolitis, acute gastroenteritis in children St. Petersburg 2013.Epidemiological,...
Tuberculosis imposes a major burden of death and disease on the human population. Each year, tens millions people become infected with Mycobacterium tuberculosis , several develop clinical disease, more than 2 million die (1)(2). The successful treatment depends heavily timely diagnostics selection an adequate strategy. Use genotyping tools based molecular techniques decreases time necessary for detection drug resistance in M. from weeks to few days or even less, patient’s regimen can be...
Category A select agents continue to be major threat human population both as naturally occurring diseases and potential weapon of bioterrorists. Anthrax botulism are probably the most threatening have virtually uncontrolled natural reservoirs from which they can isolated propagated. Available specific antitoxin therapy is outdated; its efficiency questionable well safety reactogenic or human-derived components used in treatment. Highly sensitive toxin detection techniques still not...
Immobilization of anti- B. anthracis monoclonal antibodies (MAbs) on latex microparticles was studied, the optimal load these MAbs determined to be 20 µg for 50 µl stock suspension. The highest sensitivity agglutination test observed 1E6 MAbs. Latex suspensions with immobilized were lyophilized. Their and specificity shown highly competitive those liquid diagnosticum Bacillus spores detection constructed basis lyophilized reagents, developed approved regulatory documentation that included...