A5035711444- Mycobacterium research and diagnosis
- Tuberculosis Research and Epidemiology
- Infectious Diseases and Mycology
- Genomics and Phylogenetic Studies
- Infectious Diseases and Tuberculosis
- Diagnosis and treatment of tuberculosis
- Quinazolinone synthesis and applications
- Actinomycetales infections and treatment
- Pharmacological Effects of Natural Compounds
- Antibiotic Resistance in Bacteria
- Pneumocystis jirovecii pneumonia detection and treatment
- Helicobacter pylori-related gastroenterology studies
- Ginseng Biological Effects and Applications
- Pneumonia and Respiratory Infections
- Bacteriophages and microbial interactions
- Inhalation and Respiratory Drug Delivery
- Antibiotics Pharmacokinetics and Efficacy
- Fungal Biology and Applications
- Whipple's Disease and Interleukins
- Antibiotic Use and Resistance
- Lichen and fungal ecology
- Immunodeficiency and Autoimmune Disorders
- Plant Disease Resistance and Genetics
- Innovative Microfluidic and Catalytic Techniques Innovation
- Fungal Infections and Studies
Istituto di Ricovero e Cura a Carattere Scientifico San Raffaele
2014-2024
Vita-Salute San Raffaele University
2014-2024
Istituti di Ricovero e Cura a Carattere Scientifico
2014-2024
University Hospital Leipzig
2021
Leipzig University
2021
Azienda Ospedaliero-Universitaria Careggi
2004-2019
Hospital General Universitario Gregorio Marañón
2019
Centre National de la Recherche Scientifique
2019
CEA Paris-Saclay
2019
Commissariat à l'Énergie Atomique et aux Énergies Alternatives
2019
Abstract Background The Direct Repeat locus of the Mycobacterium tuberculosis complex (MTC) is a member CRISPR (Clustered regularly interspaced short palindromic repeats) sequences family. Spoligotyping widely used PCR-based reverse-hybridization blotting technique that assays genetic diversity this and useful both for clinical laboratory, molecular epidemiology, evolutionary population genetics. It easy, robust, cheap, produces highly diverse portable numerical results, as result...
A significant knowledge gap exists concerning the geographical distribution of nontuberculous mycobacteria (NTM) isolation worldwide. To provide a snapshot NTM species distribution, global partners in NTM-Network European Trials Group (NET) framework ( www.ntm-net.org ), branch Tuberculosis Network (TB-NET), provided identification results total number patients 2008 whom were isolated from pulmonary samples. From these data, we visualised relative different found per continent and country....
Nontuberculous mycobacteria (NTM) represent over 190 species and subspecies, some of which can produce disease in humans all ages affect both pulmonary extrapulmonary sites. This guideline focuses on adults (without cystic fibrosis or human immunodeficiency virus infection) caused by the most common NTM pathogens such as Mycobacterium avium complex, kansasii, xenopi among slowly growing abscessus rapidly NTM. A panel experts was carefully selected leading international respiratory medicine...
Nontuberculous mycobacteria (NTM) represent over 190 species and subspecies, some of which can produce disease in humans all ages affect both pulmonary extrapulmonary sites. This guideline focuses on adults (without cystic fibrosis or human immunodeficiency virus infection) caused by the most common NTM pathogens such as Mycobacterium avium complex, kansasii , xenopi among slowly growing abscessus rapidly NTM. A panel experts was carefully selected leading international respiratory medicine...
Abstract Nontuberculous mycobacteria (NTM) represent over 190 species and subspecies, some of which can produce disease in humans all ages affect both pulmonary extrapulmonary sites. This guideline focuses on adults (without cystic fibrosis or human immunodeficiency virus infection) caused by the most common NTM pathogens such as Mycobacterium avium complex, kansasii, xenopi among slowly growing abscessus rapidly NTM. A panel experts was carefully selected leading international respiratory...
Extrapulmonary tuberculosis (EPTB) accounts for more than 20% of (TB) cases. Xpert MTB/RIF (Xpert) (Cepheid, Sunnyvale, CA, USA) is a fully automated amplification system, which excellent results in the diagnosis pulmonary TB highly endemic countries have been recently reported. We aimed to assess performance system diagnosing EPTB low incidence setting. investigated with large number consecutive extrapulmonary clinical specimens (1,476, corresponding 1,068 patients) including both...
The taxonomic position of members the Mycobacterium abscessus complex has been subject intensive investigation and, in some aspects confusion, recent years as a result varying approaches to genetic data interpretation. Currently, former species massiliense and bolletii are grouped together subsp. bolletii. They differ greatly, however, M. functional erm(41) gene that confers inducible resistance macrolides, primary therapeutic antimicrobials for abscessus, while is non-functional....
Abstract Mycobacterium tuberculosis and leprae have remained, for many years, the primary species of genus clinical microbiological interest. The other members genus, referred to as nontuberculous mycobacteria (NTM), long been underinvestigated. In last decades, however, number reports linking various NTM with human diseases has steadily increased treatment difficulties emerged. Despite availability whole genome sequencing technologies, limited effort devoted genetic characterization...
The possibility that the strains included within Mycobacterium avium complex (MAC), but not belonging either to M. or intracellulare , may be members of undescribed taxa, has already been questioned by several taxonomists. A very homogeneous cluster 12 characterized identical nucleotide sequences both in 16S rDNA and 16S–23S internal transcribed spacer was investigated. Similar strains, previously reported literature, had assigned species on basis similarity group MAC intermediates. However,...
ABSTRACT The BACTEC MGIT 960 instrument is a fully automated system that exploits the fluorescence of an oxygen sensor to detect growth mycobacteria in culture. Its performance was compared those radiometric 460 and egg-based Lowenstein-Jensen medium. An identical volume sample inoculated different media, incubation carried out for 6 weeks with automatic systems 8 on solid media. A total 2,567 specimens obtained from 1,631 patients were cultured parallel. Mycobacteria belonging nine taxa...
The names 'Mycobacterium abscessus subsp. abscessus' and massiliense', proposed by Leao et al. (2009, J Clin Microbiol 47, 2691-2698), cannot be validly published. purpose of this report is to provide a description in accordance with the Rules Bacteriological Code (1990 Revision). Moreover, proposal name massiliense' contrary Rule 38 correct taxon, at rank subspecies, Mycobacterium bolletii comb. nov. A an emended are also given.
Screening for active tuberculosis (TB) and latent TB infection (LTBI) is mandatory prior to the initiation of tumour necrosis factor-alpha inhibitor therapy. However, no agreement exists on best strategy detecting LTBI in this population. The aim present study was analyse performance tuberculin skin test (TST) QuantiFERON-TB Gold in-tube (QFT-GIT) detection subjects with immunomediated inflammatory diseases (IMID). TST QFT-GIT were prospectively performed 398 consecutive IMID subjects, 310...
ABSTRACT An outbreak of postsurgical infections caused by rapidly growing mycobacteria has been ongoing in Brazil since 2004. The degrees similarity the rpoB and hsp65 sequences from clinical isolates corresponding both Mycobacterium massiliense M. bolletii type strains were above accepted limit for interspecies variability, leading to conflicting identification results. Therefore, an extensive characterization members chelonae-M. abscessus group was carried out. , chelonae immunogenum a...