A5014104516- Mycobacterium research and diagnosis
- Tuberculosis Research and Epidemiology
- Infectious Diseases and Mycology
- Infectious Diseases and Tuberculosis
- Genomics and Phylogenetic Studies
- Antibiotic Resistance in Bacteria
- Pneumocystis jirovecii pneumonia detection and treatment
- Antimicrobial Resistance in Staphylococcus
- Diagnosis and treatment of tuberculosis
- Bacterial biofilms and quorum sensing
- Viral gastroenteritis research and epidemiology
- Cancer therapeutics and mechanisms
- Clostridium difficile and Clostridium perfringens research
- Biochemical and Molecular Research
- HIV/AIDS drug development and treatment
- Infections and bacterial resistance
- Immunodeficiency and Autoimmune Disorders
- Fungal Biology and Applications
- Pediatric health and respiratory diseases
- Bacteriophages and microbial interactions
- Pneumonia and Respiratory Infections
- Ginseng Biological Effects and Applications
- Genomics and Rare Diseases
- Plant Pathogenic Bacteria Studies
- Actinomycetales infections and treatment
Istituti di Ricovero e Cura a Carattere Scientifico
2014-2024
Istituto di Ricovero e Cura a Carattere Scientifico San Raffaele
2014-2024
Vita-Salute San Raffaele University
2014-2024
San Raffaele University of Rome
2014-2022
IRCCS Ospedale San Raffaele
2019-2022
Tunis El Manar University
2019
University of Monastir
2019
The taxonomic position of members the Mycobacterium abscessus complex has been subject intensive investigation and, in some aspects confusion, recent years as a result varying approaches to genetic data interpretation. Currently, former species massiliense and bolletii are grouped together subsp. bolletii. They differ greatly, however, M. functional erm(41) gene that confers inducible resistance macrolides, primary therapeutic antimicrobials for abscessus, while is non-functional....
Molecular diagnostics are considered the most promising route to achieving rapid, universal drug susceptibility testing for
The Comprehensive Resistance Prediction for Tuberculosis: an International Consortium (CRyPTIC) presents here a data compendium of 12,289 Mycobacterium tuberculosis global clinical isolates, all which have undergone whole-genome sequencing and had their minimum inhibitory concentrations to 13 antitubercular drugs measured in single assay. It is the largest matched phenotypic genotypic dataset M. date. Here, we provide summary detailing breadth collected, along with description how isolates...
The objective of this study was to develop standardized protocols for rapid delamanid drug susceptibility testing (DST) using the colorimetric resazurin microtitre assay (REMA) and semi-automated BACTEC™ MGIT™ 960 system (MGIT) by establishing breakpoints that accurately discriminate between resistance Mycobacterium tuberculosis delamanid. MICs were determined MGIT, REMA solid agar method 19 pre-characterized strains. MIC distribution then established a panel clinical strains never exposed...
Staphylococcus aureus strains are now regarded as zoonotic agents. In pastoral settings where human-animal interaction is intimate, multi-drug resistant microorganisms have become an emerging issue of public health concern. The study S. prevalence, antimicrobial resistance and clonal lineages in humans, animals food African has great relevance, taking into consideration the high diversity ethnicities, cultures habits that determine lifestyle people. Little known about milk carriage...
We report on the first six cases of acquired resistance to bedaquiline in Pakistan. Seventy sequential isolates from 30 drug-resistant-tuberculosis patients bedaquiline-containing regimens were retrospectively tested for by MIC testing and detection mutations relevant genes. documented failing therapy that developed specific Rv0678 had increased MICs associated with cross-resistance clofazimine during treatment. This study underlines relevance surveillance programs following introduction new drugs.
In this work we compared the most frequently used Klebsiella pneumoniae typing methods: PFGE, cgMLST and coreSNP. We evaluated discriminatory power of three methods to confirm or exclude nosocomial transmission on K. strains isolated from January December 2017, in framework routine surveillance for multidrug-resistant organisms at San Raffaele Hospital, Milan. results different epidemiological investigation. Our showed that coreSNP are more discriminant than both approaches suitable...
Using a hybrid long-read sequencing approach, we aimed to fully characterise four extensively-drug resistant (XDR) hypervirulent Klebsiella pneumoniae isolates, one of which represented the first strain isolated in Italy co-expressing NDM-1/5 and OXA-48 carbapenemases.Whole-genome was performed using Illumina Oxford Nanopore Technology platforms. An assembly pipeline used recover structures both chromosome plasmids.Multilocus sequence typing (MLST) showed that these strains belonged...
ABSTRACT Clostridium difficile infection (CDI) became a public health problem for the global spreading of so-called hypervirulent PCR ribotypes (RTs) 027 and 078, associated with increases in transmission severity disease. However, especially Europe, several RTs are prevalent, concept hypervirulence is currently debated. We investigated toxin resistance profiles genetic relatedness 312 C. strains isolated large Italian teaching hospital during 5-year period. evaluated role CDI-related...
The emergence of multidrug resistant Staphylococcus aureus strains, including methicillin (MRSA), is a global concern. Treatment bacterial infections in Uganda's health care settings largely empirical, rarely accompanied by laboratory confirmation. Here we show the burden, characteristics MRSA and epidemiology Panton-Valentine Leukocidin (PVL) positive strains asymptomatic carriers pastoral households south-west Uganda.Nasal swabs from 253 participants were cultured following standard...
Whole Genome Sequencing (WGS) is becoming affordable with overall costs comparable to other tests currently in use perform the diagnosis of drug-resistant tuberculosis (TB) and cluster analysis. The WGS approach allows an “all-in-one” providing results on expected sensitivity strains, genetic background, epidemiological data, indication risk laboratory cross-contamination. Although ideal, from direct diagnostic specimen not yet standardized, date two most promising approaches are early...
Clostridium difficile infection (CDI), one of the most common hospital-acquired infections, is increasing in incidence and severity with emergence diffusion hypervirulent strains. CDI precipitated by antibiotic treatment that destroys equilibrium gut microbiota. Human α-defensin 5 (HD5), abundant enteric antimicrobial peptide, a key regulator microbiota homeostasis, yet it still unknown if C. difficile, which successfully evades killing other host microbicidal peptides, susceptible to HD5....
Four strains isolated in Iran from pulmonary specimens of unrelated patients are proposed as representative a novel Mycobacterium species. Similarity, at the phenotypic level, with kansasii is remarkable photochromogenic yellow pigmentation colonies being salient feature. They differ, however, genotypically this species and present unique sequences 16S rRNA, hsp65 rpoB genes. The average nucleotide identity genome-to-genome distance fully support status an independent name for persicum sp....
Background: The possibility of inter-human transmission Mycobacterium abscessus in cystic fibrosis centres has been recently hypothesized suggesting the need for molecular characterization strains isolated from such patients. Materials and Methods: One hundred forty one isolates M. grown sputum samples 29 patients with were genotyped resorting to variable number tandem repeats (VNTR) determination whole genome sequencing (WGS). Results: Out VNTR profiles, 15 unique same while seven shared by...
Abstract To investigate transmission of drug-resistant strains Mycobacterium tuberculosis in Tunisia, we performed whole-genome sequencing on 46 multidrug-resistant isolated during 2012–2016. Core-genome multilocus sequence typing grouped 30 (65.2%) into 3 clusters, indicating extensive recent and Haarlem clone predominance. Whole-genome might help public health services undertake appropriate control actions.
Abstract In a 12-month nationwide study on the prevalence of drug-resistant tuberculosis (TB) in Lebanon, we identified 3 multidrug-resistant cases and extensively TB refugees, migrants, 1 Lebanon resident. Enhanced diagnostics, particularly major destinations for asylum seekers, migrant workers, can inform treatment decisions may help prevent spread TB.
Two mycobacterial strains with close similarity to the Mycobacterium tuberculosis complex (MTBC) were isolated from cutaneous lesions of patients in USA and Italy. At phenotypic level, similarities MTBC included slow growth rate, rough morphotype unpigmented colonies nearly identical high-performance liquid chromatography profiles mycolic acids. In contrast MTBC, niacin- nitrate-negative, catalase-positive both at 68 °C semi-quantitative tests. The clinical isolates more closely related M....
Background Molecular genotyping of M.tuberculosis is an important laboratory tool in the context emerging drug resistant TB. The standard 24-loci MIRU-VNTR typing includes PCR amplification followed by detection and sizing fragments using capillary electrophoresis on automated sequencers or agarose gels. QIAxcel Advanced system might offer a cost-effective medium-throughput alternative. Methods Performance characteristics platform for 24 VNTR loci panel was evaluated at two centres total 140...
Abstract Drug susceptibility testing of M. tuberculosis is rooted in a binary susceptible/resistant paradigm. Whilst there are considerable advantages measuring the minimum inhibitory concentrations (MICs) panel drugs for an isolate it n ecessary to measure epidemiological cutoff values (ECOFF/ECVs) permit comparison with qualitative data. Here we present ECOFF/ECVs 13 anti-TB compounds, including bedaquiline and delamanid, derived from 20,637 clinical isolates collected by 14 laboratories...