David Santos-Lázaro
A5031775310- Mycobacterium research and diagnosis
- Tuberculosis Research and Epidemiology
- Antibiotic Resistance in Bacteria
- Pneumonia and Respiratory Infections
- Cancer therapeutics and mechanisms
- Medical Device Sterilization and Disinfection
- Biochemical and Molecular Research
- Bacterial biofilms and quorum sensing
- Biosimilars and Bioanalytical Methods
- vaccines and immunoinformatics approaches
Instituto Nacional de Salud
2020-2024
Centro Peruano de Estudios Sociales
2022
Molecular diagnostics are considered the most promising route to achieving rapid, universal drug susceptibility testing for
The Comprehensive Resistance Prediction for Tuberculosis: an International Consortium (CRyPTIC) presents here a data compendium of 12,289 Mycobacterium tuberculosis global clinical isolates, all which have undergone whole-genome sequencing and had their minimum inhibitory concentrations to 13 antitubercular drugs measured in single assay. It is the largest matched phenotypic genotypic dataset M. date. Here, we provide summary detailing breadth collected, along with description how isolates...
The World Health Organization has a goal of universal drug susceptibility testing for patients with tuberculosis. However, molecular diagnostics to date have focused largely on first-line drugs and predicting susceptibilities in binary manner (classifying strains as either susceptible or resistant). Here, we used multivariable linear mixed model alongside whole genome sequencing quantitative microtiter plate assay relate genomic mutations minimum inhibitory concentration (MIC) 15,211...
Bedaquiline is a core drug for the treatment of multidrug-resistant tuberculosis; however, understanding resistance mechanisms poor, which hampering rapid molecular diagnostics. Some bedaquiline-resistant mutants are also cross-resistant to clofazimine. To decipher bedaquiline and clofazimine determinants, we combined experimental evolution, protein modelling, genome sequencing, phenotypic data.
Abstract Tuberculosis (TB), caused by Mycobacterium tuberculosis , is one of the deadliest infectious diseases worldwide. Multidrug and extensively drug-resistant strains are making disease control difficult, exhausting treatment options. New anti-TB drugs bedaquiline (BDQ), delamanid (DLM) pretomanid (PTM) have been approved for multi-drug resistant TB, but there increasing resistance to them. Nine genetic loci strongly linked identified ( mmpR5 atpE pepQ BDQ; ddn fgd1 fbiA fbiB fbiC fbiD...
Whole Genome Sequencing (WGS) is a promising tool in the global fight against tuberculosis (TB). The aim of this study was to evaluate use WGS routine conditions for detection drug resistance markers and transmission clusters multidrug-resistant TB hot-spot area Peru. For this, 140 drug-resistant Mycobacterium strains from Lima Callao were prospectively selected processed through (GenoType MTBDR sl BACTEC MGIT) workflows, simultaneously. Resistance determined accordance with World Health...
Abstract Peru has the highest burden of multidrug-resistant tuberculosis in Americas region. Since 1999, annual number extensively drug-resistant (XDR-TB) Peruvian cases been increasing, becoming a public health challenge. The objective this study was to perform genomic characterization Mycobacterium strains obtained from patients with XDR-TB diagnosed 2011 2015 Peru. Whole genome sequencing (WGS) performed on 68 different regions 58 (85.3%) came most populated districts Lima and Callao....
Abstract Drug susceptibility testing of M. tuberculosis is rooted in a binary susceptible/resistant paradigm. Whilst there are considerable advantages measuring the minimum inhibitory concentrations (MICs) panel drugs for an isolate it n ecessary to measure epidemiological cutoff values (ECOFF/ECVs) permit comparison with qualitative data. Here we present ECOFF/ECVs 13 anti-TB compounds, including bedaquiline and delamanid, derived from 20,637 clinical isolates collected by 14 laboratories...
Abstract Background Tuberculosis (TB) is a communicable, preventable and curable disease caused by the bacterium Mycobacterium tuberculosis (MTB). Peru amongst 30 countries with highest burden of multidrug-resistant (MDR-TB) worldwide. In fight against drug-resistant tuberculosis, UKMYC6 microdilution plate was developed validated CRyPTIC project. The objective study to evaluate use broth (BMD) methodology for susceptibility testing MTB strains in Peru. Methods isolated between 2015 2018...
Evaluation of resistance to antituberculosis drugs is routinely performed with genotypic or phenotypic methods; however, discordance can be seen between these different methodologies. Our objective was identify mutations that could explain discordant results in the evaluation susceptibility rifampicin and isoniazid molecular methods, using whole genome sequencing (WGS). Peruvian strains showing sensitive GenoType MTBDR plus v2.0 test resistant proportions agar-plaque for rifampin were...
To determine the genetic structure of drug-resistant strains Mycobacterium tuberculosis that circulated throughout Peru during years 2011-2015, by using haplotypes obtained from a line probe assay.A total 6589 samples were admitted to Instituto Nacional de Salud for routine diagnosis GenoType® MTBDRplus v2 assay analyzed study period. Resistant created concatenating 21 polymorphic sites evaluated genes assay; and association analysis was carried out with phenotypes 7H10 agar ratio method.The...
ABSTRACT Peru is amongst the 30 countries with highest burden of multidrug-resistant tuberculosis worldwide. In fight against drug-resistant tuberculosis, UKMYC6 microtiter plate was developed and validated by CRyPTIC project. Our objective to evaluate use broth microdilution for susceptibility testing Mycobacterium (MTB) strains in Peru. 496 nationally-representative MTB determined routine agar proportion method (APM) were selected. MICs 13 anti-tuberculosis drugs each strain using plates...