A5003691586- Tuberculosis Research and Epidemiology
- Mycobacterium research and diagnosis
- Parasitic Diseases Research and Treatment
- RNA and protein synthesis mechanisms
- Malaria Research and Control
- Parasite Biology and Host Interactions
- Genomics and Phylogenetic Studies
- Insect symbiosis and bacterial influences
- Parasites and Host Interactions
- Mosquito-borne diseases and control
- Enzyme Production and Characterization
- Cancer therapeutics and mechanisms
- Reproductive Health and Contraception
- Phosphodiesterase function and regulation
- Biofuel production and bioconversion
- Microbial metabolism and enzyme function
- Mollusks and Parasites Studies
- Synthesis and Catalytic Reactions
- Medical Malpractice and Liability Issues
- Maternal and Perinatal Health Interventions
London School of Hygiene & Tropical Medicine
2019-2024
Universidad de Salamanca
2017
San Antonio College
2014
Abstract Background Gluconacetobacter diazotrophicus Pal5 is an endophytic diazotrophic bacterium that lives in association with sugarcane plants. It has important biotechnological features such as nitrogen fixation, plant growth promotion, sugar metabolism pathways, secretion of organic acids, synthesis auxin and the occurrence bacteriocins. Results third to be completely sequenced. Its genome composed a 3.9 Mb chromosome 2 plasmids 16.6 38.8 kb, respectively. We annotated 3,938 coding...
Abstract The genetics underlying tuberculosis (TB) pathophysiology are poorly understood. Human genome-wide association studies have failed so far to reveal reproducible susceptibility loci, attributed in part the influence of Mycobacterium ( Mtb ) bacterial genotype on outcome infection. Several found associations human genetic polymorphisms with phylo-lineages, but analysing genome-genome interactions needed. By implementing a phylogenetic tree-based -to-human analysis for 714 TB patients...
Angiostrongylus chabaudi (arrows) on the endothelium of right ventricle a wildcat in Northern Greece
Abstract Tuberculosis (TB), caused by Mycobacterium tuberculosis , is one of the deadliest infectious diseases worldwide. Multidrug and extensively drug-resistant strains are making disease control difficult, exhausting treatment options. New anti-TB drugs bedaquiline (BDQ), delamanid (DLM) pretomanid (PTM) have been approved for multi-drug resistant TB, but there increasing resistance to them. Nine genetic loci strongly linked identified ( mmpR5 atpE pepQ BDQ; ddn fgd1 fbiA fbiB fbiC fbiD...
Introduction Around 10% of the coding potential Mycobacterium tuberculosis is constituted by two poorly understood gene families, pe and ppe loci, thought to be involved in host-pathogen interactions. Their repetitive nature high GC content have hindered sequence analysis, leading exclusion from whole-genome studies. Understanding genetic diversity pe/ppe families essential facilitate their translation into tools for prevention treatment. Methods To investigate 169 / genes, we performed a...
Abstract With >1 million associated deaths in 2020, human tuberculosis (TB) caused by the bacteria Mycobacterium remains one of deadliest infectious diseases. A plethora genomic tools and bioinformatics pipelines have become available recent years to assist whole genome sequencing M. tuberculosis. The Oxford Nanopore Technologies (ONT) portable sequencer is a promising platform for cost-effective application clinics, including personalizing treatment through detection drug...
Abstract Human tuberculosis disease (TB), caused by Mycobacterium ( Mtb) , is a complex disease, with spectrum of outcomes. Genomic, transcriptomic and methylation studies have revealed differences between Mtb lineages, likely to impact on transmission, virulence drug resistance. However, so far no integrated sequence-based genomic, characterisation across common set samples, which critical understand how DNA sequence affect RNA expression and, ultimately, pathogenesis. Here we perform such...
Abstract The genomic diversity of Plasmodium malariae malaria parasites is understudied, partly because infected individuals tend to present with low parasite densities, leading difficulties in obtaining sufficient DNA for genome analysis. Selective whole amplification (SWGA) increases the relative levels pathogen a clinical sample, but has not been adapted P. parasites. Here we design customized SWGA primers which successfully amplify extracted directly from unprocessed blood samples...
Abstract Since dirofilariosis caused by Dirofilaria immitis is a vector-borne disease, its distribution depends on environmental conditions as well demographic factors and the management of pets humans. In province Salamanca (west-central Spain) disease has been known for many years, appearing in an area with extensive irrigated crops along Tormes river. Because recent changes have occurred this area, present study carried out aim monitoring prevalence canine population area. For that...
Cyclic nucleotide–dependent phosphodiesterases (PDEs) play essential roles in regulating the malaria parasite life cycle, suggesting that they may be promising antimalarial drug targets. PDE inhibitors are used safely to treat a range of noninfectious human disorders. Here, we report three subseries fast-acting and potent Plasmodium falciparum PDEβ block asexual blood-stage development also active against clinical isolates. Two inhibitor have transmission-blocking activity by targeting PDEs...
Objectives: To increase the rate of obtaining informed consent for family planning procedures (insertion intrauterine devices and subcutaneous contraceptive implants) Study design: Uncontrolled pre- post- intervention design Setting: S. Julião Family Health Unit. Participants: Patients in FHU who visited Unit insertion an device or implant. Methods: The team designed form August 2012 but usage rates were found to be low (62.5%). Focus groups organized propose quality improvement strategies...