A5010118534- Parasite Biology and Host Interactions
- Vector-borne infectious diseases
- Parasites and Host Interactions
- Bacterial Identification and Susceptibility Testing
- Parasitic Diseases Research and Treatment
- Viral Infections and Vectors
- Parasitic Infections and Diagnostics
- Infective Endocarditis Diagnosis and Management
- Salmonella and Campylobacter epidemiology
- Mosquito-borne diseases and control
- Parasitic infections in humans and animals
- Reproductive tract infections research
- Antimicrobial Resistance in Staphylococcus
- Antibiotic Resistance in Bacteria
- Helminth infection and control
- Amoebic Infections and Treatments
- Burkholderia infections and melioidosis
- Viral gastroenteritis research and epidemiology
- Mycobacterium research and diagnosis
- Vibrio bacteria research studies
- Insect symbiosis and bacterial influences
- Antifungal resistance and susceptibility
- Leptospirosis research and findings
- Streptococcal Infections and Treatments
- Bacterial biofilms and quorum sensing
Bernhard Nocht Institute for Tropical Medicine
2014-2025
Swiss Tropical and Public Health Institute
2018-2024
University of Basel
2018-2024
University Medical Center Hamburg-Eppendorf
2015-2019
Universität Hamburg
2015-2019
Institute of Medical Microbiology and Hygiene
2006-2017
University Children's Hospital Tübingen
2017
University Medical Center Freiburg
2017
Eye Center
2017
Freie Universität Berlin
2017
Introduction: The increasing incidence of infections caused by extended-spectrum beta-lactamase (ESBL)-producing Escherichia coli in sub-Saharan Africa is serious concern. Studies from countries with a highly industrialized poultry industry suggest the production-food-consumer chain as potential transmission route. In Africa, integrated studies at this human-animal interface are still missing. Aim: To determine molecular epidemiology ESBL-producing E. intestinal tract humans and rural Ghana....
There is paucity of data regarding the geographical distribution, incidence, and phylogenetics multi-drug resistant (MDR) Salmonella Typhi in sub-Saharan Africa. Here we present a phylogenetic reconstruction whole genome sequenced 249 contemporaneous S. isolated between 2008-2015 11 African countries, context 2,057 global genomic framework. Despite broad genetic diversity, majority organisms (225/249; 90%) belong to only three genotypes, 4.3.1 (H58) (99/249; 40%), 3.1.1 (97/249; 39%), 2.3.2...
ABSTRACT Real-time PCR and fluorescence in situ hybridization (FISH) were evaluated as rapid methods for the diagnosis of bacterial meningitis compared to standard diagnostic procedures. For PCR, a LightCycler approach was chosen, implementing eubacterial specific assays most relevant bacteria. FISH, similar probe set containing probes composed published newly designed probes. Both by use cerebrospinal fluid (CSF) samples from patients with suspected meningitis. all microscopy-...
Angiostrongylus chabaudi (arrows) on the endothelium of right ventricle a wildcat in Northern Greece
Acute schistosomiasis constitutes a rare but serious condition in individuals experiencing their first prepatent Schistosoma infection. To circumvent costly and time-consuming diagnostics, an early rapid diagnosis is required. So far, classic diagnostic tools such as parasite microscopy or serology lack considerable sensitivity at this stage of validate the use blood based real-time polymerase chain reaction (PCR) test for detection DNA patients with acute who acquired infection various...
During weeks 32–33, 2013, 24 cases of cryptosporidiosis were notified in the city Halle (annual mean 2008–2012: 9 cases). We investigated outbreak to identify source and recommend control measures, considering that between 23–25 river Saale which flows through centre overflowed floodplain, parts damaged sewage systems. defined a case as resident with gastroenteritis, Cryptosporidium-positive stool disease onset 27 47. In case–control study among kindergarten children, we compared controls...
Rodents are important reservoirs for a large number of zoonotic pathogens. We examined the occurrence 11 viral, bacterial, and parasitic agents in rodent populations Austria, including three different hantaviruses, lymphocytic choriomeningitis virus, orthopox Leptospira spp., Borrelia Rickettsia Bartonella Coxiella burnetii, Toxoplasma gondii. In 2008, 110 rodents four species (40 Clethrionomys glareolus, 29 Apodemus flavicollis, 26 sylvaticus, 15 Microtus arvalis) were trapped at two rural...
Cutaneous dirofilariosis is a canine mosquito-borne zoonosis that can cause larva migrans disease in humans. Dirofilaria repens considered an emerging pathogen occurring with high prevalence Mediterranean areas and many parts of tropical Asia. In Hong Kong, second species, Candidatus hongkongensis, has been reported. The present study aimed to compare mitochondrial genomes from these parasites obtain population genetic information.Complete were obtained by PCR Sanger sequencing or ILLUMINA...
Little is known about the presence of vector-borne bacteria in southwest Madagascar. Anthropogenic alteration natural habitats represents an important driver for emergence new diseases. Especially involvement livestock and involuntary maintaining invasive synanthropic animals (particularly rats) facilitate disease transmission from wildlife to humans associated vice versa. The dissemination or acquisition ectoparasites most likely regions where human/wildlife contact increasing. In 2016...
Abstract Nodding syndrome is a neglected, disabling and potentially fatal epileptic disorder of unknown aetiology affecting thousands individuals mostly confined to Eastern sub-Saharan Africa. Previous studies have identified multiple associations—including Onchocerca volvulus, antileiomodin-1 antibodies, vitamin B6 deficiency measles virus infection—yet, none proven causal. We conducted case-control study children with early-stage nodding (symptom onset <1 year). Cases controls were...
Phenotypic identification of gram-negative bacteria from Cystic Fibrosis (CF) patients carries a high risk misidentification. Therefore, we compared the results biochemical by API 20NE with 16S rRNA gene sequencing in 88 gram-negative, oxidase-positive rods, other than morphologically and biochemically typical P. aeruginosa, respiratory secretions CF patients. The allowed correct bacterial species 15 out (17%) isolates investigated. Agreement between was only an result classified as...
ABSTRACT Chlamydiae are important pathogens of humans and animals but diagnosis chlamydial infections is still hampered by inadequate detection methods. Fluorescence in situ hybridization (FISH) using rRNA-targeted oligonucleotide probes widely used for the investigation uncultured bacteria complex microbial communities has recently also been shown to be a valuable tool rapid various bacterial clinical specimens. Here we report on development evaluation hierarchic probe set specific...
Chlamydiae are a unique group of obligate intracellular bacteria comprising important pathogens vertebrates as well symbionts free-living amoebae. Although there is ample molecular evidence for huge diversity and wide distribution chlamydiae in nature, environmental currently represented by only few isolates. This paper reports the recovery novel chlamydia strain from activated sludge co-cultivation with Acanthamoeba sp. The recovered UV-7 showed characteristic morphology chlamydial...
Dirofilaria repens, a filarial nematode of dogs and other carnivores, can accidentally infect humans. Clinical symptoms are usually restricted to subcutaneous nodule containing single infertile parasite. Here, we report case D. repens infection with gravid worm the patient's concomitant meningoencephalitis aphasia.
Knowledge of the potential vector role Culicidae mosquitoes in Germany is very scanty, and until recently it was generally assumed that they are not involved transmission anthroponotic or zoonotic pathogens this country. However, anticipated changes course global warming globalization may alter their status.We conducted a molecular mass screening for filarial parasites using mitochondrial 12S rRNA-based real-time PCR.No causing disease humans such as Dirofilaria spp. were detected about...
Due to considerable differences in pathogenicity, Haemophilus influenzae, H. parainfluenzae and haemolyticus have be reliably discriminated routine diagnostics. Retrospective analyses suggest frequent misidentifications of commensal as influenzae. In a multi-center approach, we assessed the suitability fluorescence situ hybridization (FISH) matrix-assisted laser-desorption-ionization time-of-flight mass-spectrometry (MALDI-TOF-MS) for identification species level.A strain collection 84 spp....
Methicillin-resistant Staphylococcus aureus (MRSA) clones pose a significant threat to hospitalised patients because the bacteria can be transmitted by asymptomatic carriers within healthcare facilities. To date, nothing is known about prevalence of S. and MRSA among workers in Madagascar. The objective our study was examine clonal epidemiology nasal non-medical University students Antananarivo, This cross sectional screened swabs taken from for aureus. Multiplex PCR performed identify...
Rarely, zoonotic Taenia species other than solium cause human cysticercosis. The larval stages are morphologically often indistinguishable. We therefore investigated 12 samples of suspected cysticercosis cases at the molecular level and surprisingly identified one crassiceps serialis (coenurosis) infection, which were caused by tapeworm larvae normally infecting rodents sheep via eggs released from foxes dogs.
Non-typhoidal Salmonella (NTS) cause the majority of bloodstream infections in Ghana, however mode transmission and source invasive NTS Africa are poorly understood. This study compares from water sources rural Ghana. Blood hospitalised, febrile children samples drinking were analysed for spp. Strains serotyped to trace possible epidemiological links between human water-derived isolates.. Antibiotic susceptibility testing was performed, In 2720 blood culture samples, 165 (6%) isolated. S....
Leptospirosis is among the most important zoonotic diseases in (sub-)tropical countries. The research objective was to evaluate accuracy of Serion IgM ELISA EST125M against Microscopic Agglutination Test (MAT = imperfect reference test); assess its ability diagnose acute leptospirosis infections and detect previous exposure leptospires an endemic setting. In addition, estimate overall Leptospira spp. seroprevalence Wiwa indigenous population North-East Colombia. We analysed serum samples...