A5046401519- Reproductive tract infections research
- Syphilis Diagnosis and Treatment
- Vector-borne infectious diseases
- Viral Infections and Vectors
- Bacterial Infections and Vaccines
- Vector-Borne Animal Diseases
- Antibiotic Resistance in Bacteria
- Mosquito-borne diseases and control
- Systemic Lupus Erythematosus Research
- Dermatological diseases and infestations
- Monoclonal and Polyclonal Antibodies Research
- Adolescent Sexual and Reproductive Health
- Antimicrobial Resistance in Staphylococcus
- Microbial infections and disease research
- Mycobacterium research and diagnosis
- Bacterial biofilms and quorum sensing
- Streptococcal Infections and Treatments
- Urinary Tract Infections Management
- Clostridium difficile and Clostridium perfringens research
- Bacterial Identification and Susceptibility Testing
- Rabies epidemiology and control
- Pneumonia and Respiratory Infections
- Blood groups and transfusion
- Genital Health and Disease
- Reproductive Health and Contraception
Amsterdam University Medical Centers
2019-2025
University of Amsterdam
1999-2025
Public Health Service of Amsterdam
2016-2025
National Institute for Public Health and the Environment
2022-2024
Office of Infectious Diseases
2024
Czech Academy of Sciences, Institute of Microbiology
2024
Amsterdam UMC Location University of Amsterdam
1998-2023
GGD Amsterdam
2011-2023
OLVG
2011-2023
University Medical Center
2022-2023
Borrelia burgdorferi sensu lato has been subdivided into three genospecies: B. stricto, garinii, and group VS461. Sixty-eight isolates cultured from patients 26 strains ticks were characterized with use of SDS-PAGE, western blotting, rRNA gene restriction analysis. Fifty-seven 58 obtained the skin 70 who had erythema migrams or acrodermatitis chronica atrophicans VS461, whereas genotype remaining strain was unidentifiable. Of 10 CSF (n = 3) 7) 20 extracutaneous symptoms Lyme borreliosis,...
Traditional methods for molecular epidemiology of Neisseria gonorrhoeae are suboptimal. Whole-genome sequencing (WGS) offers ideal resolution to describe population dynamics and predict infer transmission antimicrobial resistance, can enhance infection control through linkage with epidemiological data. We used WGS, in conjunction linked phenotypic data, the gonococcal 20 European countries. aimed detail changes resistance levels (and reasons these changes) strain distribution (with a focus...
To clarify the taxonomic status of two recently described Borrelia genomic groups, groups VS116 and M19, three group strains eight M19 isolated from Ixodes ricinus ticks in Switzerland, The Netherlands, United Kingdom were characterized. PCR-restriction fragment length polymorphism (RFLP) analysis 5S-23S intergenic spacer amplicon, rRNA gene restriction analysis, 16S sequence randomly amplified polymorphic DNA (RAPD) fingerprinting, sodium dodecyl sulfate-polyacrylamide gel electrophoresis...
ABSTRACT Serology and nucleic acid amplification are the main diagnostic tools for diagnosis of Mycoplasma pneumoniae infection. Since no reference standard is generally accepted, serologic assays M. have not been evaluated on a broad scale. In this study, 12 commercially available (for immunoglobulin G [IgG] IgM) complement fixation test (CFT) were by using DNA detection real-time PCR as “gold standard.” The tested Platelia EIA (Bio-Rad), SeroMP (Savyon), Serion classic (Virion/Serion),...
A curated Web-based user-friendly sequence typing tool based on antimicrobial resistance determinants in Neisseria gonorrhoeae was developed and is publicly accessible (https://ngstar.canada.ca). The N. Sequence Typing for Antimicrobial Resistance (NG-STAR) molecular scheme uses the DNA sequences of 7 genes (penA, mtrR, porB, ponA, gyrA, parC, 23S rRNA) associated with to β-lactam antimicrobials, macrolides, or fluoroquinolones. NG-STAR entire penA sequence, combining historical nomenclature...
BackgroundGenomic surveillance using quality-assured whole-genome sequencing (WGS) together with epidemiological and antimicrobial resistance (AMR) data is essential to characterise the circulating Neisseria gonorrhoeae lineages their association patient groups (defined by demographic factors). In 2013, European gonococcal population was characterised genomically for first time. We describe in 2018 identify emerging or vanishing associated AMR characteristics of patients, elucidate recent...
BackgroundRegular quality-assured whole-genome sequencing linked to antimicrobial resistance (AMR) and patient metadata is imperative elucidate the shifting gonorrhoea epidemiology, both nationally internationally. We aimed examine gonococcal population in European Economic Area (EEA) 2020, emerging disappearing lineages associated with AMR metadata, compare 2013 2018 data, explain changes epidemiology.MethodsIn this retrospective genomic surveillance study, we analysed consecutive isolates...
A global increase in the incidence of invasive group Streptococcus (iGAS) infections was observed after lifting COVID-19 related restrictions 2022, and type M1UK dominated many countries. After seasonal declines iGAS during summer 2023, simultaneous, rapid expansion a previously rare emm 3.93 seen beginning November, increasing to 20% all cases England 60% Netherlands within 4 months. emm3.93 associated with children 6-17 years age increased risk for pneumonia or pleural empyema meningitis...
Borrelia burgdorferi-related isolates were tested for their sensitivity to normal human serum (NHS) and ability activate complement. By dark-field microscopy, electron subsurface plating, it was shown that exposure of a garinii isolate 10% or more NHS resulted in immobilization, blebbing, killing the spirochetes. These effects mediated by complement, since they not seen after heat treatment NHS, presence EDTA, an agammaglobulinemic serum. All seven B. type 5 6 all four VS116/M19 strains...
Encephalitozoon species are important pathogens in human immunodeficiency virus-infected patients. However, immunocompetent persons, little is known about infections, mainly because of the lack reliable diagnostic tests. To improve diagnosis, three serologic techniques that use intestinalis as antigen were developed: an ELISA, immunofluorescence technique (IFAT), and a counterimmunoelectrophoresis (CIE) method. The response against E. was studied sera from 300 Dutch blood donors 276 pregnant...
Borrelia burgdorferi, the agent of Lyme disease, is transmitted by ticks. During transmission from tick to host, spirochetes are delivered with saliva, which contains salivary protein Salp15. Salp15 has been shown protect against B. burgdorferi-specific antibodies. We now show that both Ixodes ricinus and scapularis protects serum-sensitive isolates burgdorferi sensu lato complement-mediated killing. I. showed strong protective effects compared those Deposition terminal C5b C9 (one molecule...
Repeated exposure of rabbits and other animals to ticks results in acquired resistance or immunity subsequent tick bites is partially elicited by antibodies directed against antigens. In this study we demonstrate the utility a yeast surface display approach identify salivary antigens that react with tick-immune serum. We constructed an Ixodes scapularis nymphal gland library screened nymph-immune rabbit sera identified five Four these proteins, designated P8, P19, P23 P32, had predicted...
To elucidate the genome-based epidemiology and phylogenomics of azithromycin-resistant (MIC >2 mg/L) Neisseria gonorrhoeae strains collected in 2009-14 Europe clarify azithromycin resistance mechanisms.Seventy-five 4 to >256 N. isolates 17 European countries during were examined using antimicrobial susceptibility testing WGS.Thirty-six multi-antigen sequence typing STs five phylogenomic clades, including 4-22 from several per clade, identified. The target mutation A2059G (Escherichia coli...
CXCL13 in cerebrospinal fluid (CSF) could be an important component for diagnosing Lyme neuroborreliosis (LNB). Levels of intrathecal were determined 58 LNB patients and 210 controls; sensitivity was 88% specificity 89% (cutoff, 250 pg CXCL13/ml CSF). Elevated levels can aid the diagnosis LNB, but should interpreted with care.
Abstract Objectives Novel antimicrobials for treatment of gonorrhoea are imperative. The first-in-class spiropyrimidinetrione zoliflodacin is promising and currently in an international Phase 3 randomized controlled clinical trial (RCT) uncomplicated gonorrhoea. We evaluated the vitro activity genetic conservation target (GyrB) other potential resistance determinants among 1209 consecutive Neisseria gonorrhoeae isolates obtained from 25 EU/European Economic Area (EEA) countries 2018 compared...