A5038149250- Mycobacterium research and diagnosis
- Tuberculosis Research and Epidemiology
- Infectious Diseases and Mycology
- Genomics and Phylogenetic Studies
- Bacteriophages and microbial interactions
- Actinomycetales infections and treatment
- Ginseng Biological Effects and Applications
- Bacterial Identification and Susceptibility Testing
- Vibrio bacteria research studies
- Bacillus and Francisella bacterial research
- Pharmacological Effects of Natural Compounds
- Plant Pathogens and Fungal Diseases
- Infectious Diseases and Tuberculosis
- Leptospirosis research and findings
- Quinazolinone synthesis and applications
- Microbial infections and disease research
- Plant Pathogenic Bacteria Studies
- Legionella and Acanthamoeba research
- Infective Endocarditis Diagnosis and Management
- Aquaculture disease management and microbiota
- Coccidia and coccidiosis research
- Antibiotic Resistance in Bacteria
- Animal Disease Management and Epidemiology
- Fungal Infections and Studies
- Genetic diversity and population structure
University of Manitoba
2000-2024
Manitoba Health
2018-2024
Diagnostic Services Manitoba
2016-2018
Saskatchewan Disease Control Laboratory
2012-2017
The University of Texas Health Science Center at Tyler
2007-2011
Munson Medical Center
2011
McGill University Health Centre
2005-2009
Montreal General Hospital
2006-2009
McGill University
2006-2009
Health Canada
2000-2007
ABSTRACT Invasive fungal disease often plays an important role in the morbidity and mortality of immunocompromised patients. The poor sensitivity current blood culture histological practices has led to development highly sensitive specific molecular techniques, such as PCR. Sequence variability internal transcribed spacer 2 (ITS2) region fungi is potentially useful rapid accurate diagnosis clinical isolates. PCR with fungus-specific primers targeted toward conserved sequences 5.8S 28S...
The taxonomic position of members the Mycobacterium abscessus complex has been subject intensive investigation and, in some aspects confusion, recent years as a result varying approaches to genetic data interpretation. Currently, former species massiliense and bolletii are grouped together subsp. bolletii. They differ greatly, however, M. functional erm(41) gene that confers inducible resistance macrolides, primary therapeutic antimicrobials for abscessus, while is non-functional....
ABSTRACT The use of the 16S rRNA gene for identification nontuberculous mycobacteria (NTM) provides a faster and better ability to accurately identify them in addition contributing significantly discovery new species. Despite their associated problems, many rely on public sequence databases comparisons. To best evaluate taxonomic status NTM species submitted our reference laboratory, we have created database by sequencing 121 American Type Culture Collection strains encompassing 92...
ABSTRACT Amikacin is a major drug used for the treatment of Mycobacterium avium complex (MAC) disease, but standard laboratory guidelines susceptibility testing are not available. This study presents in vitro amikacin MICs 462 consecutive clinical isolates MAC using broth microdilution assay. Approximately 50% had 8 μg/ml, and 86% ≤16 μg/ml. Of eight (1.7%) with 64 five an MIC 32 μg/ml on repeat testing. Ten (2.1%) initial >64 which seven (1.5%) These 16S rRNA gene A1408G mutation...
Recent studies have shown that respiratory isolates from pulmonary disease patients and household water/biofilm of Mycobacterium avium could be matched by DNA fingerprinting. To determine if this is true for intracellulare, water sources 36 with complex (MAC) lung were evaluated. MAC three published included 37 additional also Species identification was done initially using nonsequencing methods confirmation internal transcribed spacer (ITS) and/or partial 16S rRNA gene sequencing. M....
Current methods for identification of Mycobacterium spp. rely upon time-consuming phenotypic tests, mycolic acid analysis, and narrow-spectrum nucleic probes. Newer approaches include PCR sequencing technologies. We evaluated the MicroSeq 500 16S ribosomal DNA (rDNA) bacterial kit (Applied Biosystems, Foster City, Calif.) its ability to identify isolates. The is based on first bp rRNA gene. One hundred nineteen mycobacterial isolates (94 clinical 25 reference strains) were identified using...
ABSTRACT The Mycobacterium avium complex consists of epidemiologically distinct subsets. classification these subsets is complicated by a number factors, including the ambiguous results obtained with phenotypic and genetic assays recent appreciation that human avian strains appear to be distinct. In previous work, sequencing based on 441-bp portion hsp65 gene has proven efficiently classify isolates within genus but provides low resolution for distinguishing among members M. complex....
Mycobacterium avium comprises organisms that share the same species designation despite considerable genomic and phenotypic variability. To determine degree nature of variability between subspecies strains M. avium, we used multilocus sequencing analysis, studying 56 genetically diverse included all described subspecies. In total, 8,064 bp sequence from 10 gene loci were studied, with 205 (2.5%) representing variable positions. The majority (149/205) these variations found among subsp....
Abstract Background In the past decade, availability of complete genome sequence data has greatly facilitated comparative genomic research aimed at addressing genetic variability within species. More recently, analysis across species become feasible, especially in genera where sequencing projects multiple have been initiated. To understand genesis pathogen Mycobacterium tuberculosis a genus majority are harmless environmental organisms, we used from 16 mycobacteria to look for evidence...
The genus Leptospira currently comprises 16 named species. In addition, four unnamed hybridization groups were designated genomospecies 1, 3, 4 and 5. These represent valid species-level taxa, but not assigned names in the original description by Brenner et al. [Int J Syst Bacteriol 49, 839-858 (1999)]. To rectify this situation, it is proposed that 5 should be classified as alstonii sp. nov., vanthielii terpstrae nov. yanagawae respectively, with strains L. 79601(T) ( = ATCC BAA-2439(T)),...
ABSTRACT 16S rRNA sequence data have been used to provide a molecular basis for an accurate system identification of members the genus Mycobacterium . Previous studies shown that species demonstrate high levels (>94%) similarity and this method cannot differentiate between all species, i.e., M. gastri kansasii In present study, we recA gene as alternative sequencing target in order complement sequence-based genetic identification. The genes 30 were amplified by PCR, sequenced, compared...
Mycobacterium porcinum is a rarely encountered rapidly growing (RGM). We identified M. from 24 patients at Galveston university hospital (University of Texas Medical Branch) over 5-year period. was considered pathogen in 11 (46%) infected patients, including 4 with community-acquired disease. Retrospective patient data were collected, and water samples cultured. Molecular analysis isolates, clustered clinical 15 unrelated control strains performed. Among ice tap water, 63% positive for RGM,...
Summary Matrix‐assisted laser desorption ionisation‐time of flight mass spectrometry ( MALDI ‐ TOF MS ) is commonly used by clinical microbiology laboratories to identify bacterial pathogens and yeasts, but not for the identification moulds. Recent progress in extraction protocols composition comparative libraries support potential application mould laboratories. We evaluated performance Bruker Microflex™ instrument (Billerica, MA , USA isolates reference strains moulds using 3 libraries,...
ABSTRACT The Bacillus genus is a large heterogeneous group in need of an efficient method for species differentiation. To determine the current validity sequence-based identification and provide contemporary data, PCR sequencing 500-bp product encompassing V1 to V3 regions 16S rRNA gene were undertaken using 65 83 type strains this genus. This region proved discriminatory between most (70.0 100% similarity), exceptions being clinically relevant B. cereus anthracis as well nonpathogenic...
The clinical profile of nontuberculous mycobacteria (NTM) has been raised by the human immunodeficiency virus and AIDS pandemic. Different laboratory techniques, often molecular based, are available to facilitate rapid accurate identification NTM. expense these advanced techniques questioned. At National Reference Center for Mycobacteriology Health Sciences Center, University Manitoba, in Winnipeg, Canada, we performed a direct cost analysis commercial DNA probe-negative (Gen-Probe, Inc.,...
ABSTRACT DNA degradation (which results in a smear pattern) occurs with almost 50% of Mycobacterium abscessus strains during pulsed-field gel electrophoresis (PFGE). We assessed the potential benefit using thiourea-containing buffer M. by studying 69 isolates not previously typeable PFGE (i.e., those pattern). Random (epidemiologically unrelated) that were (no included as controls. Genomic was digested DraI, XbaI, and AseI. gels run regular without 100 μM thiourea. All generated patterns had...
ABSTRACT The Mycobacterium avium species consists of a group organisms that are genetically related but phenotypically diverse, with certain variants presenting clear differences in terms their host association and disease manifestations. ability to distinguish between these subtypes is relevance for accurate diagnosis control programs. Using comparative genomics approach, we have uncovered large sequence polymorphisms are, respectively, absent from bird-type M. isolates cattle types sheep...
ABSTRACT Mycobacterium avium comprises genetically related yet phenotypically distinct subspecies. Consistent with their common origin, whole-genome sequence comparisons have revealed extensive synteny among M. organisms. However, the sequenced strains also display numerous regions of heterogeneity that likely contribute to diversity individual Starting from a phylogenetic framework derived by multilocus analysis, we examined distribution 25 large polymorphisms across panel defined strains....
Mycobacterium avium complex (MAC) currently comprises eight species of environmental and animal-associated, slowly-growing mycobacteria: avium, intracellulare, chimaera, colombiense, arosiense , bouchedurhonense, marseillense timonense. In humans, MAC organisms are responsible for opportunistic infections whose unique epidemiology remains poorly understood, in part due to the lack a genotyping method applicable all species. this study we developed multispacer sequence typing (MST),...
ABSTRACT Mycobacterium terrae complex has been recognized as a cause of tenosynovitis, with M. and nonchromogenicum reported the primary etiologic pathogens. The molecular taxonomy causing tenosynovitis not established despite approximately 50 previously cases. We evaluated 26 isolates associated or osteomyelitis recovered between 1984 2014 from 13 states, including 5 in 1991 by nonmolecular methods. belonged to three validated species, one new proposed two novel related strains. majority...
ABSTRACT Bacteremia continues to result in significant morbidity and mortality, particularly patients who are immunocompromised. Currently, with suspected bacteremia empirically administered broad-spectrum antibiotics, as definitive diagnosis relies upon the use of blood cultures, which impose delays limitations pathogen identification. To address growth-based identification, sequence variability 16S rRNA gene bacteria was targeted for rapid identification bacterial pathogens isolated...