A5049209185- Virus-based gene therapy research
- Pluripotent Stem Cells Research
- Genomics and Phylogenetic Studies
- Animal Genetics and Reproduction
- Molecular Biology Techniques and Applications
- Fish Biology and Ecology Studies
- RNA modifications and cancer
- Fish Ecology and Management Studies
- Retinal Diseases and Treatments
- Gene expression and cancer classification
- Genetic diversity and population structure
- Morphological variations and asymmetry
- Evolution and Paleontology Studies
- Bayesian Methods and Mixture Models
- Bioinformatics and Genomic Networks
- Forensic Entomology and Diptera Studies
- Statistical Distribution Estimation and Applications
- Cancer-related molecular mechanisms research
- Parasite Biology and Host Interactions
- Retinal and Macular Surgery
- Diptera species taxonomy and behavior
- Glaucoma and retinal disorders
- Ophthalmology and Visual Impairment Studies
- Optical Coherence Tomography Applications
- Corneal surgery and disorders
University of Malaya
2014-2024
National University of Singapore
2010
We explored the gut microbiota profile among HIV-infected individuals with diverse immune recovery profiles following long-term suppressive ART and investigated relationship between altered bacteria markers of dysfunction. The rectal swabs from 26 20 HIV-uninfected controls were examined. Patients classified as suboptimal responders, sIR (n = 10, CD4 T-cell <350 cells/ul) optimal oIR 16, >500 after a minimum 2 years on ART. Canonical correlation analysis(CCA) multiple regression modelling...
The drug discovery and development pipeline is a long arduous process that inevitably hampers rapid development. Therefore, strategies to improve the efficiency of are urgently needed enable effective drugs enter clinic. Precision medicine has demonstrated genetic features cancer cells can be used for predicting response, emerging evidence suggest gene-drug connections could predicted more accurately by exploring cumulative effects many genes simultaneously.We developed DeSigN, web-based...
APOBEC3B is a cytosine deaminase implicated in immune response to viral infection, cancer predisposition and carcinogenesis. Germline deletion more common East Asian women confers modest risk breast both Caucasian women. Analysis of tumour samples from European descent has shown that germline associated with an increased propensity develop somatic mutations enrichment for response-related gene sets. However, this not been examined samples, where population differences genetic dietary factors...
Background. Anchors are one of the important attachment appendages for monogenean parasites. Common descent and evolutionary processes have left their mark on anchor morphometry, in form patterns shape size variation useful systematic studies. When combined with morphological molecular data, analysis morphometry can potentially answer a wide range biological questions. Materials Methods. We used data from body morphology 13 Ligophorus (Monogenea: Ancyrocephalidae) species infecting two...
Abstract Aim Freshwater planarians may have a wide geographical range despite their assumed low vagility. Found across four continents, Dugesia either an ancient origin on large palaeo landmass, followed by colonisation in different regions before continental fragmentation, or more recent and subsequent transoceanic dispersal. We seek to resolve between these two hypotheses. Location Africa, Eurasia Australasia. Taxon Genus (Platyhelminthes: Tricladida: Dugesiidae). Methods used data from...
Helminth infection and dietary intake can affect the intestinal microbiota, as well immune system. Here we analyzed relationship between fecal microbiota blood profiles of indigenous Malaysians, referred to locally Orang Asli, in comparison urban participants from capital city Malaysia, Kuala Lumpur. We found that helminth infections had a larger effect on gut microbial composition than did or profiles. Trichuris trichiura intensity also strongest association with transcriptional By...
Abstract Background Mycobacterium abscessus is a rapidly growing mycobacterium that often associated with human infections. The taxonomy of this species has undergone several revisions and still being debated. In study, we sequenced the genomes 12 M. strains used phylogenomic analysis to perform subspecies classification. Results A data mining approach was rank select informative genes based on relative entropy metric for construction phylogenetic tree. resulting tree topology similar...
Background. A common research goal in transcriptome projects is to find genes that are differentially expressed different phenotype classes. Biologists might wish validate such gene candidates experimentally, or use them for downstream systems biology analysis. Producing a coherent differential expression analysis from RNA-seq count data requires an understanding of how numerous sources variation as the replicate size, hypothesized biological effect and specific method making calls interact....
<h3>Background/Aims</h3> To compare homocysteine (Hcy) concentration in the blood plasma, vitreous and aqueous of eyes with proliferative diabetic retinopathy (PDR) against control, to investigate associations between Hcy plasma that these two groups. <h3>Methods</h3> Blood samples were collected during combined cataract pars plana vitrectomy from 20 PDR 21 patients without diabetes mellitus. was determined by chemiluminescent microparticle immunoassay. <h3>Results</h3> The mean (± standard...
In current statistical methods for calling differentially expressed genes in RNA-Seq experiments, the assumption is that an adjusted observed gene count represents unknown true count. This adjustment usually consists of a normalization step to account heterogeneous sample library sizes, and then resulting normalized counts are used as input parametric or non-parametric differential expression tests. A distribution counts, each with different probability, can result same Importantly,...
A new generalization of the logarithmic distribution (LD)—the generalized (GLD)—is introduced. The GLD is a cluster size inverse trinomial distribution, which arises in random walk problem. Major properties are derived and discussed. method first moment frequency one count maximum likelihood estimation (MLE) used for parameter estimation. Rao's score test statistic as model selection tool. Finally, we demonstrate an application fitting insect data from temperate tropical regions.
In medical, veterinary and forensic entomology, the ease affordability of image data acquisition have resulted in whole-image analysis becoming an invaluable approach for species identification. Krawtchouk moment invariants are a classical mathematical transformation that can extract local features from image, thus allowing subtle species-specific biological variations to be accentuated subsequent analyses. We extracted invariant binarised wing images 759 male fly specimens Calliphoridae,...
Head and neck cancer (HNC)–derived cell lines represent fundamental models for studying the biological mechanisms underlying development precision therapies. However, mining genomic information of HNC cells from available databases requires knowledge on bioinformatics computational skill sets. Here, we developed a user-friendly web resource exploring, visualizing, analyzing genomics commonly used lines. We populated current version GENIPAC with 44 3 studies: ORL Series, OPC-22, H Series....
Salmonid species are main actors in the Italian socio-ecological landscape of inland fisheries. We present novel data on size-age structure one remnant populations critically endangered marble trout Salmo marmoratus , which co-occurs with other stocked non-native salmonids a large glacial river Lake Maggiore basin (Northern Italy-Southern Switzerland). Like native populations, Toce River population is affected by anthropogenic introgression brown S. trutta . Our sample includes 579...
Abstract Wing shape variation has been shown to be useful for delineating forensically important fly species in two Diptera families: Calliphoridae and Sarcophagidae. Compared DNA‐based identification, the cost of geometric morphometric data acquisition analysis is relatively much lower because tools required are basic, stable softwares available. However, date, an explicit demonstration using wing identity prediction these families remains lacking. Here, from 19 homologous landmarks on left...
To report the rate of cystoid macular oedema (CMO) as detected by spectral-domain optical coherence tomography (SD-OCT) after intraoperative complication during phacoemulsification. The secondary objectives include comparing mean thickness and best-corrected visual acuity (BCVA) between those who developed postoperative CMO against did not. This is a prospective cohort study conducted in tertiary hospital July 2009 June 2010. Serial SD-OCT BCVA were performed at baseline, 1 week, 6 weeks 16...
Infection with the hepatitis B virus (HBV) may lead to an acute or chronic infection. It is generally accepted that clinical outcome of infection depends on balance between host immunity and viral survival strategies. In order persist, needs have a high rate replication some immune-escape capabilities. Hence, HBVs lacking these properties are likely be eliminated more rapidly by host, leading lower chronicity. To test this hypothesis, 177 HBV genomes from non-fulminant cases 1,149 were...