A5072620092- Tuberculosis Research and Epidemiology
- Mycobacterium research and diagnosis
- Pneumocystis jirovecii pneumonia detection and treatment
- Infectious Diseases and Tuberculosis
- Diagnosis and treatment of tuberculosis
- Animal Disease Management and Epidemiology
- Microbial infections and disease research
- Immunodeficiency and Autoimmune Disorders
- Antibiotic Resistance in Bacteria
- Bacteriophages and microbial interactions
- Pneumonia and Respiratory Infections
- vaccines and immunoinformatics approaches
- Viral Infections and Vectors
- Immune Cell Function and Interaction
- Viral Infections and Outbreaks Research
- HIV/AIDS Impact and Responses
- HIV Research and Treatment
- Trypanosoma species research and implications
- SARS-CoV-2 and COVID-19 Research
- Vector-borne infectious diseases
- Vector-Borne Animal Diseases
- Monoclonal and Polyclonal Antibodies Research
- Immune Response and Inflammation
- Infectious Diseases and Mycology
- Data-Driven Disease Surveillance
Administración Nacional de Laboratorios e Institutos de Salud
2013-2022
Consejo Nacional de Investigaciones Científicas y Técnicas
2005-2022
Centro Científico Tecnológico - San Juan
1998-2019
National Space Activities Commission
2016
Centro Científico Tecnológico - Tucumán
2009-2016
Centers for Disease Control and Prevention
2009
National Institute of Health of Thailand
2009
Queen Sirikit National Institute of Child Health
2009
Sanatorio Otamendi y Miroli
2009
Centro de Educación Médica e Investigaciones Clínicas Norberto Quirno
1979-2009
SUMMARY In the last decade, an unprecedented genetic diversity has been disclosed among Mycobacterium tuberculosis strains found worldwide. However, well-conserved genotypes seem to prevail in areas with high incidence of tuberculosis. As this may be related selective advantages, such as advanced mechanisms circumvent [M. bovis Bacille Calmette–Guerin (BCG)-induced] host defence mechanisms, we investigated influence strain on course experimental disease. Twelve M. strains, representing four...
Abstract The rise of drug-resistant strains is a major challenge to containing the tuberculosis (TB) pandemic. Yet, little known about extent resistance in early years chemotherapy and when transmission resistant on larger scale became public health issue. Here we reconstruct timeline acquisition antimicrobial during ongoing outbreak multidrug-resistant TB Argentina. We estimate that progenitor strain acquired isoniazid, streptomycin rifampicin by around 1973, indicating continuous...
One hundred fifty-three Mycobacterium bovis strains from cattle, various animal species zoos and wild parks, humans were analyzed for three different genetic markers use in the epidemiology of bovine tuberculosis. M. isolated cattle found to carry a single IS6110 element, whereas majority other animals such as antelopes, monkeys, seals harbored multiple elements, suggesting that reservoirs are separated. Because element is located at same chromosomal position, strain differentiation by...
On the basis of population genomic and phylogeographic analyses 1669 Mycobacterium tuberculosis lineage 4 (L4) genomes, we find that dispersal L4 has been completely dominated by historical migrations out Europe. We demonstrate an intimate temporal relationship between European colonial expansion into Africa Americas spread (TB). Markedly, in age antibiotics, mutations conferring antimicrobial resistance overwhelmingly emerged locally (at level nations), with minimal cross-border...
Current drug choices to treat extensively drug-resistant (XDR) tuberculosis (TB) are scarce; therefore, information on the safety, tolerability and efficacy of alternative regimens is utmost importance. The aim this study was describe management, adverse effects outcome combined treatment in a series XDR-TB patients.A retrospective performed 17 non-AIDS, pulmonary adult patients with admitted referral centre for infectious diseases Buenos Aires from 2002 through 2008. Drug susceptibility...
A steep upsurge of human immunodeficiency virus (HIV)-associated multidrug-resistant tuberculosis (MDR-TB) was recently observed at a referral treatment center in Buenos Aires City. Between January 1994 and June 1995, TB isolates resistant to least five drugs were recovered from 101 272 HIV-infected inpatients. Highly 77 patients underwent restriction fragment length polymorphism study with IS6110. After cross-contamination eliminated, single strain found have caused disease 68 history...
ABSTRACT Mycobacterium avium has become a major human pathogen, primarily due to the emergence of AIDS epidemic. Restriction fragment length polymorphism (RFLP) typing, using insertion sequence IS 1245 as probe, provides powerful tool in molecular epidemiology M. -related infections and will facilitate well-founded studies into sources animal environmental reservoirs. The standardization this technique allows computerization RFLP patterns for comparison on local level establishment DNA...
Abstract Background Mutations associated with resistance to rifampin or streptomycin have been reported for W/Beijing and Latin American Mediterranean (LAM) strain families of Mycobacterium tuberculosis . A few studies limited sample sizes separately evaluated mutations in kat G, ahp C inh genes that are isoniazid (INH) resistance. Increasing prevalence INH resistance, especially high (TB) prevalent countries is worsening the burden TB control programs, since similar transmission rates noted...
Background. The proinflammatory cytokine interleukin 17 (IL-17) plays an important role in immune responses but it is also associated with tissue-damaging inflammation. So, we evaluated the ability of Mycobacterium tuberculosis clinical isolates to induce IL-17 (TB) patients and healthy human tuberculin reactors (PPD+HD). Methods. IL-17, interferon γ (IFN-γ), 23 (IL-23) receptor expression were ex vivo cultured peripheral blood mononuclear cells from TB PPD+HD stimulated irradiated multidrug...
The tuberculosis (TB) epidemic is fueled by a parallel Human Immunodeficiency Virus (HIV) epidemic, but it remains unclear to what extent the HIV has been driver for drug resistance in Mycobacterium (Mtb). Here we assess impact of co-infection on emergence and transmission Mtb largest outbreak multidrug-resistant TB South America date. By combining Bayesian evolutionary analyses reconstruction networks utilizing new model optimized TB, find that does not significantly affect transmissibility...
Ultrastructural and immunohistochemical studies on tissues from five patients with Argentine hemorrhagic fever revealed previously undetected lesions caused by the viral infection. Two types of particle were seen in cells all organs examined. The particles had some characteristics similar to those described for arenaviruses. However, virus-like intracellular, a single membrane, apparently originated process budding into endoplasmic reticulum cisternae. Intranuclear bodies three cytopolasmic...
In Argentina, multidrug-resistant tuberculosis (MDR-TB) outbreaks emerged among hospitalized patients with AIDS in the early 1990s and thereafter disseminated to immunocompetent community. Epidemiological, bacteriological, genotyping data allowed identification of certain MDR Mycobacterium outbreak strains, such as so-called strain M Haarlem lineage Ra Latin America Mediterranean lineage. current study, we evaluated immune responses induced by strains peripheral blood mononuclear cells from...
The frequency of the Beijing genotype Mycobacterium tuberculosis as a cause (TB) in South America was determined by analyzing genotypes strains isolated from patients that had been diagnosed with disease between 1997 and 2003 seven countries subcontinent. In total, 19 1,202 (1.6%) TB cases carried isolates, including 11 185 Peru (5.9%), five 512 Argentina (1.0%), two 252 Brazilian (0.8%), one 166 Paraguay (0.6%) none samples obtained Chile (35), Colombia (36) Ecuador (16). Except for were...
Tuberculosis remains the single largest infectious disease with 10 million new cases and two deaths that are estimated to occur yearly, more than any time in history. The intracellular replication of Mycobacterium tuberculosis (Mtb) its spread from lungs other sites before development adaptive immune responses. Dendritic cells (DC) professional antigen-presenting whose maturation is critical for onset protective response against may vary depending on nature cell wall Mtb strain. Here, we...
We present a picture of the biodiversity Mycobacterium tuberculosis in Paraguay, an inland South American country harboring 5 million inhabitants with notification rate 38/100,000. A total 220 strains collected throughout 2003 were classified by spoligotyping into 79 different patterns. Spoligopatterns 173 matched 51 shared international types (SITs) already updated version SpolDB4, global spoligotype database at Pasteur Institute, Guadeloupe. Our study contributed to 13 new SITs and 15...