Darı́o Garcı́a de Viedma
A5006005815- Tuberculosis Research and Epidemiology
- Mycobacterium research and diagnosis
- Infectious Diseases and Tuberculosis
- SARS-CoV-2 and COVID-19 Research
- Diagnosis and treatment of tuberculosis
- SARS-CoV-2 detection and testing
- COVID-19 Clinical Research Studies
- Respiratory viral infections research
- Pneumocystis jirovecii pneumonia detection and treatment
- Pneumonia and Respiratory Infections
- Bacteriophages and microbial interactions
- Antibiotic Resistance in Bacteria
- Polyomavirus and related diseases
- Influenza Virus Research Studies
- Viral gastroenteritis research and epidemiology
- vaccines and immunoinformatics approaches
- Bacillus and Francisella bacterial research
- Bacterial Identification and Susceptibility Testing
- Genomics and Phylogenetic Studies
- Evolution and Genetic Dynamics
- RNA and protein synthesis mechanisms
- CRISPR and Genetic Engineering
- Bacterial Genetics and Biotechnology
- Antimicrobial Resistance in Staphylococcus
- Bacterial biofilms and quorum sensing
Hospital General Universitario Gregorio Marañón
2016-2025
Centro de Investigación Biomédica en Red de Enfermedades Respiratorias
2016-2025
Instituto de Salud Carlos III
2022-2025
Fundación Seimc-Gesida
2025
Centro de Investigación Biomédica en Red
2017-2024
Instituto de Biomedicina de Valencia
2019-2024
Madrid Health Service
2024
Fundación para el Fomento de la Investigación Sanitaria y Biomédica de la Comunitat Valenciana
2019-2021
Universitat de València
2019-2021
Centro de Investigación Biomédica en Red de Epidemiología y Salud Pública
2019-2021
Abstract Background The Direct Repeat locus of the Mycobacterium tuberculosis complex (MTC) is a member CRISPR (Clustered regularly interspaced short palindromic repeats) sequences family. Spoligotyping widely used PCR-based reverse-hybridization blotting technique that assays genetic diversity this and useful both for clinical laboratory, molecular epidemiology, evolutionary population genetics. It easy, robust, cheap, produces highly diverse portable numerical results, as result...
<ns3:p>Late in 2020, two genetically-distinct clusters of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) with mutations biological concern were reported, one the United Kingdom and South Africa. Using a combination data from routine surveillance, genomic sequencing international travel we track dispersal lineages B.1.1.7 B.1.351 (variant 501Y-V2). We account for potential biases surveillance efforts by including passenger volumes location where lineage was first London Africa...
<ns3:p>Late in 2020, two genetically-distinct clusters of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) with mutations biological concern were reported, one the United Kingdom and South Africa. Using a combination data from routine surveillance, genomic sequencing international travel we track dispersal lineages B.1.1.7 B.1.351 (variant 501Y-V2). We account for potential biases surveillance efforts by including passenger volumes location where lineage was first London Africa...
Abstract Background There is a paucity of knowledge on the long-term outcome in patients diagnosed with COVID-19. We describe cohort constellation symptoms occurring four weeks after diagnosis causing different degrees reduced functional capacity. Although hypothesis have been proposed to explain this condition like persistent immune activation or immunological dysfunction, date, no physiopathological mechanism has identified. Consequently, there are therapeutic options besides symptomatic...
It has been accepted that the infection by Mycobacterium tuberculosis (M. tuberculosis) can be more heterogeneous than considered. The emergence of clonal variants caused microevolution events leading to population heterogeneity is a phenomenon largely unexplored. Until now, we could only superficially analyze this standard fingerprinting (RFLP and VNTR).In study applied whole genome sequencing for in-depth analysis scale both at intrapatient interpatient scenarios.We found amount variation...
Abstract Mismatch repair (MMR) is a near ubiquitous pathway, essential for the maintenance of genome stability. Members MutS and MutL protein families perform key steps in mismatch correction. Despite major importance this MutS–MutL are absent almost all Actinobacteria many Archaea. However, these organisms exhibit rates spectra spontaneous mutations similar to MMR-bearing species, suggesting existence an alternative canonical MutS–MutL-based MMR. Here we report that Mycobacterium smegmatis...
In a patient on immunosuppressant treatment, SARS-CoV-2 RNA was documented in different extra-respiratory samples over several months the absence of positive determinations upper respiratory samples. Whole-genome sequencing these showed acquisition single-nucleotide polymorphisms time, suggesting viral evolution and thus viability.
ABSTRACT We evaluated 117 isolates of Mycobacterium tuberculosis for susceptibility to linezolid by the proportion and E-test methods. Linezolid showed high in vitro activity, with all strains inhibited ≤1 μg drug per ml. MICs were at least 4 dilutions lower than their equivalents standard method.
A monkeypox virus (MPXV) outbreak has been ongoing worldwide since May 2022. The role of specimens other than skin lesions for MPXV diagnosis is unknown. We evaluated 140 different clinical by real-time PCR. highest positivity rates (97%) were from any part the body, followed plasma, pharyngeal and anal swabs. Testing multiple sites may improve sensitivity reduce false-negative test results.
IntroductionMycobacterium caprae is a member of the Mycobacterium tuberculosis complex (MTBC) not routinely identified to species level. It lacks specific clinical features presentation and may therefore be as causative agent tuberculosis. Use whole genome sequencing (WGS) in investigation family microepidemic Almería, Spain, unexpectedly involvement M. caprae.AimWe aimed evaluate presence additional unidentified cases determine magnitude this occurrence.MethodsFirst-line characterisation...
To evaluate the efficacy of highly active antiretroviral therapy (HAART) in 12 patients with AIDS-associated progressive multifocal leukoencephalopathy (PML).The diagnosis PML was established by brain biopsy six and neuroimaging findings PCR detection JC virus cerebrospinal fluid (CSF) patients. We also studied 13 consecutive AIDS biopsy-proven cared for same institution before HAART available. Eleven group eight control received intravenous arabinoside cytosine cycles.With HAART, median...
ABSTRACT The emergence of resistance to antituberculosis drugs is a relevant matter worldwide, but the retrieval antibiograms for Mycobacterium tuberculosis severely delayed when phenotypic methods are used. Genotypic allow earlier detection resistance, although conventional approaches cumbersome or lack sensitivity specificity. We aimed design new real-time PCR method detect rifampin (RIF)- and isoniazid (INH)-resistant M. strains in single reaction tube. First, we characterized resistant...
Three patients with progressive multifocal leukoencephalopathy (PML) treated highly active antiretroviral therapy (HAART) worsened clinically and radiologically. At the time of deterioration all three had reduced HIV viraemia increased CD4 cell counts. Brain biopsy in disclosed PML marked perivascular lymphoplasmacytic infiltration. We reviewed slides 28 brain biopsies diagnostic PML. Inflammatory changes were observed four out nine on HAART one 19 not HAART. reactions against several...
ABSTRACT The Latin American-Mediterranean (LAM) family of Mycobacterium tuberculosis is believed to be the cause ∼15% cases worldwide. Previously, we defined a prevalent sublineage LAM in Brazil by single characteristic genomic deletion designated RD Rio . Using Brazilian strains, pinpoint an Ag85C 103 nucleotide polymorphism (SNP) (screened restriction fragment length [RFLP] analysis) that correctly identified all strains. Importantly, strains concomitantly possessed RD174 deletion. These...
During the last few years, PCR-based methods have been developed to simplify and reduce time required for genotyping Mycobacterium tuberculosis (MTB) by standard approaches based on IS6110-Restriction Fragment Length Polymorphism (RFLP). Of these, MIRU-12-VNTR (Mycobacterial interspersed repetitive units- variable number of tandem repeats) (MIRU-12) has considered a good alternative. Nevertheless, some limitations discrepancies with RFLP, which are minimized if technique is complemented...
We present our experience in patients with hematologic malignancy and Pseudomonas aeruginosa infection treated ceftolozane-tazobactam. performed a single-center case-control study comparing P. ceftolozane-tazobactam (study group) similar not (control to assess safety efficacy.
<ns3:p><ns3:bold>Background: </ns3:bold>Lineage 1 (L1) and 3 (L3) are two lineages of the<ns3:italic> Mycobacterium tuberculosis</ns3:italic> complex (MTBC) causing tuberculosis (TB) in humans. L1 L3 prevalent around the rim Indian Ocean, region that accounts for most world’s new TB cases. Despite their relevance this region, remain understudied.</ns3:p><ns3:p> <ns3:bold>Methods: </ns3:bold>We analyzed 2,938 2,030 whole genome sequences originating from 69 countries. We reconstructed...
A successful Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) variant, B.1.1.7, has recently been reported in the UK, causing global alarm. Most likely, new variant emerged a persistently infected patient, justifying special focus on these cases. Our aim this study was to explore certain clinical profiles involving severe immunosuppression that may help explain prolonged persistence of viable viruses. We present three severely immunosuppressed cases (A, B, and C) with history...
ABSTRACT We conducted a population-based molecular epidemiological study of tuberculosis (TB) in Madrid, Spain (2002 to 2004), define transmission patterns and factors associated with clustering. particularly focused on examining how the increase TB cases among immigrants recent years (2.8% 1997 1999 36.2% during current study) was modifying patterns. Mycobacterium isolates obtained from patients living nine districts Madrid (1,459,232 inhabitants) were genotyped. The case rate foreign-born...