A5026352573- Tuberculosis Research and Epidemiology
- Mycobacterium research and diagnosis
- Infectious Diseases and Tuberculosis
- Fermentation and Sensory Analysis
- Bacterial Genetics and Biotechnology
- Probiotics and Fermented Foods
- Evolution and Genetic Dynamics
- Bacterial biofilms and quorum sensing
- Diagnosis and treatment of tuberculosis
- Antibiotic Resistance in Bacteria
- Bacteriophages and microbial interactions
- Enzyme Production and Characterization
- Horticultural and Viticultural Research
- Immunodeficiency and Autoimmune Disorders
- Plant-Microbe Interactions and Immunity
- Wine Industry and Tourism
- Polysaccharides Composition and Applications
- Microbial Community Ecology and Physiology
- Protein Structure and Dynamics
- Plant Pathogenic Bacteria Studies
- Chronic Lymphocytic Leukemia Research
- Olfactory and Sensory Function Studies
- Clostridium difficile and Clostridium perfringens research
- Pneumocystis jirovecii pneumonia detection and treatment
- Biofuel production and bioconversion
Universitat Rovira i Virgili
2020-2021
Universidad de Málaga
2019-2020
Instituto de Hortofruticultura Subtropical y Mediterránea "La Mayora"
2019-2020
Consejo Superior de Investigaciones Científicas
2019
Centro de Investigación Biomédica en Red de Enfermedades Respiratorias
2011-2017
Hospital General Universitario Gregorio Marañón
2010-2017
Universidad Complutense de Madrid
2013-2017
Universidad San Pablo CEU
2013-2017
Centre National de la Recherche Scientifique
2015
Aix-Marseille Université
2015
Abstract Bacteria of the genera Pseudomonas and Bacillus can promote plant growth protect plants from pathogens. However, interactions between these plant-beneficial bacteria are understudied. Here, we explore interaction subtilis 3610 chlororaphis PCL1606. We show that extracellular matrix protects B. colonies infiltration by P. . The absence results in increased fluidity loss structure colony. type VI secretion system (T6SS) is activated upon contact with cells, stimulates sporulation....
It has been accepted that the infection by Mycobacterium tuberculosis (M. tuberculosis) can be more heterogeneous than considered. The emergence of clonal variants caused microevolution events leading to population heterogeneity is a phenomenon largely unexplored. Until now, we could only superficially analyze this standard fingerprinting (RFLP and VNTR).In study applied whole genome sequencing for in-depth analysis scale both at intrapatient interpatient scenarios.We found amount variation...
Bacteria can form biofilms that consist of multicellular communities embedded in an extracellular matrix (ECM). In Bacillus subtilis, the main protein component ECM is functional amyloid TasA. Here, we study further roles played by TasA B. subtilis physiology and biofilm formation on plant leaves vitro. We show ΔtasA cells exhibit a range cytological symptoms indicative excessive cellular stress leading to increased cell death. associates detergent-resistant fraction membrane, distribution...
ABSTRACT Clonally complex infections by Mycobacterium tuberculosis are progressively more accepted. Studies of their dimension in epidemiological scenarios where the infective pressure is not high scarce. Our study systematically searched for clonally (mixed than one strain and simultaneous presence clonal variants) applying mycobacterial interspersed repetitive-unit (MIRU)–variable-number tandem-repeat (VNTR) analysis to M. isolates from two population-based samples respiratory (703 cases)...
The alcohol content in wine has increased due to external factors recent decades. In reports, some non-Saccharomyces yeast species have been confirmed reduce ethanol during the alcoholic fermentation process. Thus, an efficient screening of yeasts with low yield is required broad diversity these yeasts. this study, we proposed a rapid method for selecting strains from forty-five belonging eighteen species. Single fermentations were carried out selection. Then, sequential synthetic and...
Mycobacterium bovis populations in countries with persistent bovine tuberculosis usually show a prevalent spoligotype wide geographical distribution. This study applied mycobacterial interspersed repetitive-unit-variable-number tandem-repeat (MIRU-VNTR) typing to random panel of 115 M. isolates that are representative the most frequent Iberian Peninsula, SB0121. VNTR targeted nine loci: ETR-A (alias VNTR2165), ETR-B (VNTR2461), ETR-D (MIRU4, VNTR580), ETR-E (MIRU31, VNTR3192), MIRU26...
Microbiological strategies are currently being considered as methods for reducing the ethanol content of wine. Fermentations started with a multistarter three non-Saccharomyces yeasts (Metschnikowia pulcherrima (Mp), Torulaspora delbrueckii (Td) and Zygosaccharomyces bailii (Zb)) at different inoculum concentrations. S. cerevisiae (Sc) was inoculated into fermentations 0 h (coinoculation), 48 or 72 (sequential fermentations). The microbial populations were analyzed by culture-dependent...
The use of controlled mixed inocula Saccharomyces cerevisiae and non-Saccharomyces yeasts is a common practice in winemaking, with Torulaspora delbrueckii, Lachancea thermotolerans Metschnikowia pulcherrima being the most commonly used species. Although S. usually dominant yeast at end fermentations, some species are also able to reach late stages; such may not grow culture media, which status known as viable but non-culturable (VBNC). Thus, an accurate methodology properly monitor...
The strains involved in tuberculosis outbreaks are considered highly virulent and transmissible. We analyzed the case of a patient Madrid, Spain, who was persistently infected over an 8-year period by same Beijing Mycobacterium strain. strain responsible for severe outbreak on Gran Canaria Island. provides us with unique opportunity to challenge our assumptions about M. strains. No clinical/radiological findings consistent were documented, vitro growth rate macrophages only moderate....
Molecular analysis of recurrent tuberculosis has revealed that a second episode may be caused by strain Mycobacterium other than involved in the first infection, thus indicating exogenous reinfection plays role recurrence. We focused on two aspects have received little attention. First, we evaluated whether lack methodological refinement could lead to inaccurate assignment mixed infections as reinfection, which differential selection each coinfecting strains occurred over time. used...
The role of clonal complexity has gradually been accepted in infection by Mycobacterium tuberculosis (MTB), although analyses this issue are limited. We performed an in-depth study a case recurrent MTB integrating genotyping, whole genome sequencing, analysis gene expression and infectivity vitro vivo models. Four different variants were identified from independent intrapatient evolutionary branches. One the single-nucleotide polymorphisms mapped mce3R, which encodes repressor operon...
This protocol is an optimization for metagenomic library construction from peat bog and arable soil samples. It suitable DNA inputs ranging 20 pg to 10 ng, with a fragment size of 250 bp Illumina sequencing.
Clonal complexity is increasingly accepted in Mycobacterium tuberculosis infection, including mixed infections by ≥2 strains, which usually occur settings with a high burden of and/or risk overexposure to infected patients. Mixed can hamper diagnostic procedures; obtaining an accurate antibiogram difficult when the susceptibility patterns strains differ. Here, we show how also prove challenging for other procedures, even outside where are traditionally expected. We unnoticed infection...
Abstract Bacteria can form biofilms that consist of multicellular communities embedded in an extracellular matrix (ECM). Previous studies have demonstrated genetic pathways involved biofilm formation are activated under a variety environmental conditions to enhance bacterial fitness; however, the functions individual ECM components still poorly understood. In Bacillus subtilis , main protein component is functional amyloid TasA. this study, we demonstrate beyond their well-known defect...