A5052076384- SARS-CoV-2 and COVID-19 Research
- Bacillus and Francisella bacterial research
- Poxvirus research and outbreaks
- Antimicrobial Resistance in Staphylococcus
- Herpesvirus Infections and Treatments
- Pneumocystis jirovecii pneumonia detection and treatment
- COVID-19 Clinical Research Studies
- Bacteriophages and microbial interactions
- Tuberculosis Research and Epidemiology
- Antibiotic Use and Resistance
- SARS-CoV-2 detection and testing
- Antifungal resistance and susceptibility
- CRISPR and Genetic Engineering
- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- Pneumonia and Respiratory Infections
- Nail Diseases and Treatments
- Viral gastroenteritis research and epidemiology
- Tracheal and airway disorders
- Infective Endocarditis Diagnosis and Management
- Fungal Infections and Studies
- vaccines and immunoinformatics approaches
- Antibiotic Resistance in Bacteria
- Antibiotics Pharmacokinetics and Efficacy
- PARP inhibition in cancer therapy
- Medical Imaging and Pathology Studies
Hospital General Universitario Gregorio Marañón
2020-2024
Hospital General De Segovia
2023
A monkeypox virus (MPXV) outbreak has been ongoing worldwide since May 2022. The role of specimens other than skin lesions for MPXV diagnosis is unknown. We evaluated 140 different clinical by real-time PCR. highest positivity rates (97%) were from any part the body, followed plasma, pharyngeal and anal swabs. Testing multiple sites may improve sensitivity reduce false-negative test results.
Pneumocystis jirovecii pneumonia (PJP) in immunocompromised patients entails high mortality and requires adequate laboratory diagnosis. We compared the performance of a real time-PCR assay against immunofluorescence (IFA) routine large microbiology laboratory. Different respiratory samples from HIV non-HIV-infected were included. The retrospective analysis used data September 2015 to April 2018, which included all for P. test was requested. A total 299 tested (bronchoalveolar lavage fluid (n...
The emergence of the Omicron variant SARS-CoV-2 represented a challenge to treatment COVID-19 using monoclonal antibodies. Only Sotrovimab maintained partial activity, allowing it be used in high-risk patients infected with variant. However, reports resistance mutations demand efforts better understand intra-patient resistance. A retrospective genomic analysis was conducted on respiratory samples from immunocompromised who received at our hospital between December 2021 and August 2022. study...
The present outbreak of Human Monkeypox (HMPX) that has begun in May 2022 and spread across all continents less than two months qualitative quantitative characteristics make it different from the pattern human disease previously caused by this virus. It with enormous ease, affects almost exclusively adults, behaves as a sexually transmitted focuses on very specific groups transmission conditions. high incidence city Madrid males have sex (MSM) allowed us to observe report experience first 30...
Centers for Disease Control and Prevention guidelines consider SARS-CoV-2 reinfection when sequential COVID-19 episodes occur >90 days apart. However, genomic diversity acquired over recent waves could mean previous infection provides insufficient cross-protection. We used analysis to assess the percentage of early reinfections in a sample 26 patients with 2 separated by 20-45 days. Among sampled patients, 11 (42%) had involving different variants or subvariants. Another 4 cases were...
Abstract Background SARS-CoV-2 recombinants involving the divergent Delta and Omicron lineages have been described, one of them, “Kraken” (XBB.1.5), has recently a matter concern. Recombination requires coexistence two strains in same individual. Only limited number studies focused on identification co-infections are restricted to Delta/Omicron lineages. Methods We performed systematic throughout pandemic (7609 different patients sequenced), not biassed towards involvement highly Through...
Dalbavancin is an antibiotic with activity against gram-positive bacteria that allows early discharge of patients requiring intravenous therapy. Outpatient treatment helps offset hospitalisation costs associated standard treatment. Our objective was to assess the cost disease management, including dalbavancin, in a Spanish hospital for 1 year, and hypothetical other therapeutic alternatives dalbavancin.A single-centre, observational, retrospective post-hoc analysis conducted based on...
To the Editors: Considering major outbreaks of monkeypox reported in nonendemic areas, as well changing epidemiology, there is a need to highlight difficulties diagnosing infection children, given current low index suspicion. Monkeypox zoonotic disease described 1970 endemic West-Central African countries.1 However, from May 2022 onwards, highest have been countries. Nowadays, Spain most affected country Europe.2 There are currently few publications on pediatric cases countries, and even...
Despite the proven value of applying genomic data for epidemiological purposes, commonly used high-throughput sequencing formats are not adapted to response times required intervene and finally control outbreaks. In this study, we propose a fast alternative whole-genome (WGS) track relevant microbiological strains: nanopore multiple amplicons including strain marker single nucleotide polymorphisms (SNPs). As proof concept, evaluated performance our approach offer rapid most recent public...
Abstract The emergence of the Omicron variant SARS-CoV-2 represented a challenge to treatment COVID-19 with monoclonal antibodies. Only Sotrovimab maintained partial activity, allowing it be used in high-risk patients infected variant. However, reports resistance mutations call for efforts better understand intra-patient this resistance. A retrospective genomic analysis was conducted on respiratory samples from immunocompromised who received at our hospital between December 2021 and August...
Abstract Background COVID-19 diagnosis lies on the detection of SARS-CoV-2 nasopharyngeal specimens by RT-PCR. The Xpert-Xpress assay provides results in less than one hour from specimen reception, which makes it suitable for clinical/epidemiological circumstances that require faster responses. analysis a outbreak suspected neonatology ward our institution showed Ct values obtained targeted genes Xpert were markedly different within each (N value > 20 cycles above E value). Results We...
Abstract Background The laboratory diagnosis of Pneumocystis jirovecii pneumonia (PJP) has been traditionally based on microscopy techniques, which have suboptimal sensitivity and depends the experience skills microbiologist. Molecular detection assays in PCR (Polymerase chain reaction) could improve sensitivity. Our aim was to evaluate utility real-time PJP compared with IFA (Immunofluorescence assay) performed different respiratory samples patients suspicion for routine use a clinical...