Chantal Reusken
A5066699214- Viral Infections and Vectors
- Mosquito-borne diseases and control
- SARS-CoV-2 and COVID-19 Research
- SARS-CoV-2 detection and testing
- Viral Infections and Outbreaks Research
- COVID-19 Clinical Research Studies
- Animal Virus Infections Studies
- COVID-19 epidemiological studies
- Viral gastroenteritis research and epidemiology
- Vector-borne infectious diseases
- Vector-Borne Animal Diseases
- Zoonotic diseases and public health
- Virology and Viral Diseases
- Respiratory viral infections research
- Fire effects on ecosystems
- Animal Diversity and Health Studies
- Malaria Research and Control
- Plant Virus Research Studies
- Animal Disease Management and Epidemiology
- Disaster Response and Management
- Long-Term Effects of COVID-19
- Hepatitis Viruses Studies and Epidemiology
- Travel-related health issues
- Vaccine Coverage and Hesitancy
- Infection Control and Ventilation
National Institute for Public Health and the Environment
2015-2025
Erasmus MC
2014-2025
Erasmus University Rotterdam
2013-2024
Dutch Expert Centre for Screening
2022-2024
GGD West-Brabant
2022
Public Health Service of Amsterdam
2021
Australian National University
2021
University of KwaZulu-Natal
2021
New York State Department of Health
2021
Wadsworth Center
2021
Background The ongoing outbreak of the recently emerged novel coronavirus (2019-nCoV) poses a challenge for public health laboratories as virus isolates are unavailable while there is growing evidence that more widespread than initially thought, and international spread through travellers does already occur. Aim We aimed to develop deploy robust diagnostic methodology use in laboratory settings without having material available. Methods Here we present validated workflow 2019-nCoV, its...
Abstract A new coronavirus, severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), has recently emerged to cause a human pandemic. Although molecular diagnostic tests were rapidly developed, serologic assays are still lacking, yet urgently needed. Validated needed for contact tracing, identifying the viral reservoir, and epidemiologic studies. We developed detection of SARS-CoV-2 neutralizing, spike protein–specific, nucleocapsid-specific antibodies. Using serum samples from patients...
The large virus family Paramyxoviridae includes some of the most significant human and livestock viruses, such as measles-, distemper-, mumps-, parainfluenza-, Newcastle disease-, respiratory syncytial metapneumoviruses. Here we identify an estimated 66 new paramyxoviruses in a worldwide sample 119 bat rodent species (9,278 individuals). Major discoveries include evidence origin Hendra- Nipah Africa, identification conspecific with mumps virus, detection close relatives mouse pneumonia-...
Abstract We screened fecal specimens of 4,758 bats from Ghana and 272 4 European countries for betacoronaviruses. Viruses related to the novel human betacoronavirus EMC/2012 were detected in 46 (24.9%) 185 Nycteris 40 (14.7%) Pipistrellus bats. Their genetic relatedness indicated originated
Abstract Middle East respiratory syndrome coronavirus (MERS-CoV) has caused an ongoing outbreak of severe acute tract infection in humans the Arabian Peninsula since 2012. Dromedary camels have been implicated as possible viral reservoirs. We used serologic assays to analyze 651 dromedary camel serum samples from United Arab Emirates; 151 were obtained 2003, well before onset current epidemic, and 500 2013. Recombinant spike protein–specific immunofluorescence virus neutralization tests...
Abstract A new coronavirus, SARS-CoV-2, has recently emerged to cause a human pandemic. Whereas molecular diagnostic tests were rapidly developed, serologic assays are still lacking, yet urgently needed. Validated important for contact tracing, identifying the viral reservoir and epidemiological studies. Here, we developed serological detection of SARS-CoV-2 neutralizing, spike- nucleocapsid-specific antibodies. Using serum samples from patients with PCR-confirmed infections other...
<ns3:p>Late in 2020, two genetically-distinct clusters of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) with mutations biological concern were reported, one the United Kingdom and South Africa. Using a combination data from routine surveillance, genomic sequencing international travel we track dispersal lineages B.1.1.7 B.1.351 (variant 501Y-V2). We account for potential biases surveillance efforts by including passenger volumes location where lineage was first London Africa...
<ns3:p>Late in 2020, two genetically-distinct clusters of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) with mutations biological concern were reported, one the United Kingdom and South Africa. Using a combination data from routine surveillance, genomic sequencing international travel we track dispersal lineages B.1.1.7 B.1.351 (variant 501Y-V2). We account for potential biases surveillance efforts by including passenger volumes location where lineage was first London Africa...
We obtained the full genome of Middle East respiratory syndrome coronavirus (MERS-CoV) from a camel in Qatar. This virus is highly similar to human England/Qatar 1 isolated 2012. The MERS-CoV efficiently replicated cells, providing further evidence for zoonotic potential camels.
We found serologic evidence for the circulation of Middle East respiratory syndrome coronavirus among dromedary camels in Nigeria, Tunisia, and Ethiopia. Circulation virus dromedaries across broad areas Africa may indicate that this disease is currently underdiagnosed humans outside Arabian Peninsula.
Abstract We determined and compared the humoral immune response in patients with severe (hospitalized) mild (nonhospitalized) coronavirus disease 2019 (COVID-19). Patients (n = 38) develop a robust antibody to acute respiratory syndrome 2 (SARS-CoV-2), including immunoglobulin G A antibodies. The geometric mean 50% virus neutralization titer is 1:240. SARS-CoV-2 infection was found hospital personnel 24), who developed symptoms necessitating leave of absence self-isolation, but not...
Timely detection of novel coronavirus (2019-nCoV) infection cases is crucial to interrupt the spread this virus. We assessed required expertise and capacity for molecular 2019-nCoV in specialised laboratories 30 European Union/European Economic Area (EU/EEA) countries. Thirty-eight 24 EU/EEA countries had diagnostic tests available by 29 January 2020. A coverage all was expected mid-February. Availability primers/probes, positive controls personnel were main implementation barriers.
To understand SARS-CoV-2 immunity after natural infection or vaccination, functional assays such as virus neutralising are needed. So far, to detect antibodies rely on cell-culture based either using wild type pseudotyped viruses. Such labour-intensive, require appropriate biosafety facilities and difficult standardize. Recently, a new surrogate neutralisation test (sVNT) was described that uses the principle of an ELISA measure capacity anti-SARS-CoV-2 directed against receptor binding...
The SARS-CoV-2 Omicron variant has a growth advantage over the Delta because of higher transmissibility, immune evasion or shorter serial interval. Using S gene target failure (SGTF) as indication for BA.1, we identified 908 SGTF and 1,621 non-SGTF intervals in same period. Within households, mean interval cases was 0.2-0.6 days than cases. This suggests that is partly due to
Given the emergence of SARS-CoV-2 Omicron BA.1 and BA.2 variants roll-out booster COVID-19 vaccination, evidence is needed on protection conferred by primary vaccination previous infection variant. We employed a test-negative design S-gene target failure data from community PCR testing in Netherlands 22 November 2021 to 31 March 2022 (n = 671,763). Previous infection, or both protected well against Delta infection. Protection was much lower compared Delta. similar after vaccination. Higher...
Large-scale vaccination campaigns have prevented countless hospitalizations and deaths due to COVID-19. However, the emergence of SARS-CoV-2 variants that escape from immunity challenges effectiveness current vaccines. Given this continuing evolution, an important question is when how update vaccines antigenically match circulating variants, similarly seasonal influenza viruses where antigenic drift necessitates periodic vaccine updates. Here, we studied by assessing neutralizing activity...
Infections with the Omicron SARS-CoV-2 variant are rapidly increasing worldwide. Among 174,349 SARS-CoV-2-infected individuals (≥ 12 years), we observed an increased risk of S gene target failure, predictive variant, in vaccinated (odds ratio (OR): 3.6; 95% confidence interval (CI): 3.4-3.7) and previously infected (OR: 4.2; CI: 3.8-4.7) compared naïve individuals. This suggests vaccine- or infection-induced immunity against infections is less effective than Delta variant.
Between June and September 2013, sera from 11 dromedary camels, 150 goats, 126 sheep 91 cows were collected in Jordan, where the first human Middle-East respiratory syndrome (MERS) cluster appeared 2012. All tested for MERS-coronavirus (MERS-CoV) specific antibodies by protein microarray with confirmation virus neutralisation. Neutralising found all camel while goats cattle negative. Although six reacted MERS-CoV antigen, neutralising not detected.
Hepatitis C virus (HCV) is among the most relevant causes of liver cirrhosis and hepatocellular carcinoma. Research complicated by a lack accessible small animal models. The systematic investigation viruses mammals could guide efforts to establish such models, while providing insight into viral evolutionary biology. We have assembled so-far largest collection small-mammal samples from around world, qualified be screened for bloodborne viruses, including sera organs 4,770 rodents (41...