A5067304304- HIV Research and Treatment
- Viral-associated cancers and disorders
- Respiratory viral infections research
- Viral gastroenteritis research and epidemiology
- Influenza Virus Research Studies
- Animal Virus Infections Studies
- Cytomegalovirus and herpesvirus research
- HIV/AIDS drug development and treatment
- HIV/AIDS Research and Interventions
- interferon and immune responses
- SARS-CoV-2 and COVID-19 Research
- Immune Cell Function and Interaction
- Genomics and Phylogenetic Studies
- Bacteriophages and microbial interactions
- Herpesvirus Infections and Treatments
- Viral Infections and Vectors
- Lymphoma Diagnosis and Treatment
- T-cell and B-cell Immunology
- Animal Disease Management and Epidemiology
- Viral Infections and Outbreaks Research
- Monoclonal and Polyclonal Antibodies Research
- RNA and protein synthesis mechanisms
- Gene expression and cancer classification
- COVID-19 epidemiological studies
- Viral Infections and Immunology Research
Imperial College London
2016-2025
Kymab (United Kingdom)
2016-2024
Sanofi (United Kingdom)
2022-2024
Sanofi (France)
2023-2024
The University of Texas Health Science Center at Houston
2023
Wellcome Sanger Institute
2011-2022
Uganda Virus Research Institute
2021
European Bioinformatics Institute
2020
University College London
2009-2018
National Institute of Allergy and Infectious Diseases
2018
In September 2012, the World Health Organization reported first cases of pneumonia caused by novel Middle East respiratory syndrome coronavirus (MERS-CoV). We describe a cluster health care-acquired MERS-CoV infections.Medical records were reviewed for clinical and demographic information determination potential contacts exposures. Case patients interviewed. The incubation period serial interval (the time between successive onset symptoms in chain transmission) estimated. Viral RNA was...
Human herpesvirus 8 (HHV-8, also called KSHV) is linked to the etiopathogenesis of Kaposi’s sarcoma (KS), multicentric Castleman’s disease (MCD), and primary effusion lymphoma (PEL). The universal presence HHV-8 in early KS has not yet been shown. We used a mAb (LN53) against latent nuclear antigen-1 (LNA-1) encoded by ORF73 study distribution cell types latently infected patch, plaque, nodular KS, MCD, PEL. In present <10% cells forming walls ectatic vessels. surrounding slit-like...
Serial human immunodeficiency virus type-1 (HIV-1) isolates were obtained from five individuals with acquired syndrome (AIDS) who changed therapy to 2',3'-dideoxyinosine (ddI) after at least 12 months of treatment 3'-azido-3'-deoxythymidine (zidovudine, AZT). The in vitro sensitivity ddI decreased during the following initiation, whereas AZT increased. Analysis reverse transcriptase coding region revealed a mutation associated reduced ddI. When this was present same genome as known confer...
A safe, effective, and scalable vaccine is needed to halt the ongoing SARS-CoV-2 pandemic. We describe structure-based design of self-assembling protein nanoparticle immunogens that elicit potent protective antibody responses against in mice. The vaccines display 60 spike receptor-binding domains (RBDs) a highly immunogenic array induce neutralizing titers 10-fold higher than prefusion-stabilized despite 5-fold lower dose. Antibodies elicited by RBD nanoparticles target multiple distinct...
LEDGF/p75 directly interacts with lentiviral integrase proteins and can modulate their enzymatic activities chromosomal association. A novel genetic knockout model was established that allowed us for the first time to analyze HIV-1 integration in absence of protein. Supporting a crucial role cofactor viral replication, vector reporter gene expression were significantly reduced LEDGF-null cells. Yet, processed cDNA termini normally maintained its local target DNA sequence preference during...
It is recognized that high-level resistance to 3'-azido-3'-deoxythymidine (AZT, zidovudine, or Retrovir) conferred by the presence of four mutations in human immunodeficiency virus (HIV) reverse transcriptase [RT; deoxynucleoside-triphosphate:DNA deoxynucleotidyltransferase (RNA-directed), EC 2.7.7.49] coding sequence. However, a number clinical isolates have been observed exhibit but contain only three identified (Asn-67, Arg-70, and Tyr-215). Construction molecular clone with this genotype...
The evolutionary relationships of 26 sequenced members the poxvirus family have been investigated by comparing their genome organization and gene content using DNA protein sequences for phylogenetic analyses. central region chordopoxviruses (ChPVs) is highly conserved in arrangement, except some inversions Fowlpox virus (FPV) species-specific insertions FPV Molluscum contagiosum (MCV). In 90 genes are all ChPVs, but no from near termini throughout subfamily. Inclusion two entomopoxvirus...
The order of appearance in the reverse transcriptase gene four mutations implicated development resistance to zidovudine was investigated by selective polymerase chain reaction. Serial human immunodeficiency virus isolates were studied from 18 initially asymptomatic individuals who had been treated with for 2 years. Most subjects similar patterns. first mutation occurred transiently at codon 70; its disappearance paralleled a 215. Subsequently, some individuals, 70 reappeared. During years...
We investigated a case of human infection with Middle East respiratory syndrome coronavirus (MERS-CoV) after exposure to infected camels. Analysis the whole human-derived virus and 15% camel-derived sequence yielded nucleotide polymorphism signatures suggestive cross-species transmission. Camels may act as direct source MERS-CoV infection.
BackgroundSince June, 2012, Middle East respiratory syndrome coronavirus (MERS-CoV) has, worldwide, caused 104 infections in people including 49 deaths, with 82 cases and 41 deaths reported from Saudi Arabia. In addition to confirming diagnosis, we generated the MERS-CoV genomic sequences obtained directly patient samples provide important information on transmission, evolution, origin.MethodsFull genome deep sequencing was done nucleic acid extracted PCR-confirmed clinical samples. Viral...
Community of antibodies against COVID-19 The severe acute respiratory syndrome coronavirus 2 spike protein is the basis many vaccines and a primary target neutralizing after infection. Coronavirus Immunotherapeutic Consortium (CoVIC), comprising 56 partners across world, has analyzed panel 269 monoclonal (mAbs) and, on competition profiles, sorted 186 mAbs that receptor binding domain into seven communities. Hastie et al . went to structurally analyze representative antibody used pseudovirus...
ABSTRACT The Middle East respiratory syndrome coronavirus (MERS-CoV) was first documented in the Kingdom of Saudi Arabia (KSA) 2012 and, to date, has been identified 180 cases with 43% mortality. In this study, we have determined MERS-CoV evolutionary rate, genetic variants virus and their distribution throughout Arabian peninsula, genome positions under positive selection, important features for monitoring adaptation human transmission identifying source infections. Respiratory samples from...
The SNP rs12252-C allele alters the function of interferon-induced transmembrane protein-3 increasing disease severity influenza virus infection in Caucasians, but is rare. However, much more common Han Chinese. Here we report that CC genotype found 69% Chinese patients with severe pandemic A H1N1/09 compared 25% those mild infection. Specifically, was estimated to confer a sixfold greater risk for than CT and TT genotypes. More importantly, because occurs such high frequency, its effect...
ABSTRACT Epstein-Barr virus (EBV) infects most of the world's population and is causally associated with several human cancers, but little known about how EBV genetic variation might influence infection or EBV-associated disease. There are currently no published wild-type genome sequences from a healthy individual very few genomes diseases. We have sequenced 71 geographically distinct strains cell lines, multiple types primary tumor, blood samples first saliva carrier. show that established...
Abstract Motivation: An accurate genome assembly from short read sequencing data is critical for downstream analysis, example allowing investigation of variants within a sequenced population. However, assembling virus samples, especially RNA viruses, into sequence challenging due to the combination viral population diversity and extremely uneven depth caused by amplification bias in inevitable reverse transcription polymerase chain reaction process current methods. Results: We developed new...
Analysis of clinical samples from patients with new viral infections is critical to confirm the diagnosis, specify load, and sequence data necessary for characterizing kinetics, transmission, evolution. We analyzed 112 infected recently discovered Middle East respiratory syndrome coronavirus (MERS-CoV).