Federica Mescia
A5041454738- SARS-CoV-2 and COVID-19 Research
- COVID-19 Clinical Research Studies
- Vasculitis and related conditions
- Long-Term Effects of COVID-19
- Renal Diseases and Glomerulopathies
- Otitis Media and Relapsing Polychondritis
- Cell Adhesion Molecules Research
- SARS-CoV-2 detection and testing
- Systemic Lupus Erythematosus Research
- Coagulation, Bradykinin, Polyphosphates, and Angioedema
- Complement system in diseases
- Parathyroid Disorders and Treatments
- Phagocytosis and Immune Regulation
- COVID-19 epidemiological studies
- T-cell and B-cell Immunology
- Renal and related cancers
- vaccines and immunoinformatics approaches
- Systemic Sclerosis and Related Diseases
- Machine Learning in Healthcare
- Healthcare Operations and Scheduling Optimization
- Single-cell and spatial transcriptomics
- Genetic and Kidney Cyst Diseases
- Acute Kidney Injury Research
- COVID-19 and healthcare impacts
- Immune cells in cancer
Azienda Socio Sanitaria Territoriale degli Spedali Civili di Brescia
2022-2024
University of Brescia
2021-2024
University of Cambridge
2018-2024
Addenbrooke's Hospital
2021-2024
MRC Epidemiology Unit
2023-2024
Brescia University
2022
Cambridge School
2022
University College London
2021-2022
The University of Texas Southwestern Medical Center
2018
Ospedale Papa Giovanni XXIII
2014-2015
Abstract The SARS-CoV-2 Omicron BA.1 variant emerged in 2021 1 and has multiple mutations its spike protein 2 . Here we show that the of a higher affinity for ACE2 compared with Delta, marked change antigenicity increases Omicron’s evasion therapeutic monoclonal vaccine-elicited polyclonal neutralizing antibodies after two doses. mRNA vaccination as third vaccine dose rescues broadens neutralization. Importantly, antiviral drugs remdesivir molnupiravir retain efficacy against BA.1....
Abstract Although two-dose mRNA vaccination provides excellent protection against SARS-CoV-2, there is little information about vaccine efficacy variants of concern (VOC) in individuals above eighty years age 1 . Here we analysed immune responses following with the BNT162b2 2 elderly participants and younger healthcare workers. Serum neutralization levels binding IgG or IgA after first dose were lower older individuals, a marked drop over old. Sera from showed potency B.1.1.7 (Alpha),...
Analysis of human blood immune cells provides insights into the coordinated response to viral infections such as severe acute respiratory syndrome coronavirus 2, which causes disease 2019 (COVID-19). We performed single-cell transcriptome, surface proteome and T B lymphocyte antigen receptor analyses over 780,000 peripheral mononuclear from a cross-sectional cohort 130 patients with varying severities COVID-19. identified expansion nonclassical monocytes expressing complement transcripts (CD16
The kinetics of the immune changes in COVID-19 across severity groups have not been rigorously assessed. Using immunophenotyping, RNA sequencing, and serum cytokine analysis, we analyzed serial samples from 207 SARS-CoV2-infected individuals with a range disease severities over 12 weeks symptom onset. An early robust bystander CD8+ T cell response, without systemic inflammation, characterized asymptomatic or mild disease. Hospitalized had delayed responses inflammation that was already...
Abstract Persistent symptoms following SARS-CoV-2 infection are increasingly reported, although the drivers of post-acute sequelae (PASC) COVID-19 unclear. Here we assessed 214 individuals infected with SARS-CoV-2, varying disease severity, for one year from symptom onset to determine early correlates PASC. A multivariate signature detected beyond two weeks disease, encompassing unresolving inflammation, anemia, low serum iron, altered iron-homeostasis gene expression and emerging stress...
HighlightsCombined rapid antibody + nucleic acid detection correctly diagnoses SARS-CoV-2Rapid tests detect immune responses against SARS-CoV-2 bearing D614GRapid do not cross-react with antibodies to seasonal CoVFalse positivity in finger prick blood can be very lowSummaryRapid COVID-19 diagnosis the hospital is essential, although this complicated by 30%–50% of nose/throat swabs being negative amplification testing (NAAT). Furthermore, D614G spike mutant dominates pandemic and it unclear...
The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) spike N-terminal domain (NTD) remains poorly characterized despite enrichment of mutations in this region across variants concern (VOCs). Here, we examine the contribution NTD to infection and cell-cell fusion by constructing chimeric spikes bearing B.1.617 lineage (Delta Kappa variants) NTDs generating pseudotyped lentivirus. We find that Delta on a or wild-type (WT) background increases S1/S2 cleavage efficiency virus entry,...
Abstract The biology driving individual patient responses to severe acute respiratory syndrome coronavirus 2 infection remains ill understood. Here, we developed a patient-centric framework leveraging detailed longitudinal phenotyping data and covering year after disease onset, from 215 infected individuals with differing severities. Our analyses revealed distinct ‘systemic recovery’ profiles, specific progression resolution of the inflammatory, immune cell, metabolic clinical responses. In...
Prominent early features of COVID-19 include severe, often clinically silent, hypoxia and a pronounced reduction in B cells, the latter important defence against SARS-CoV-2. This presentation resembles phenotype mice with VHL-deficient which Hypoxia-Inducible Factors are constitutively active, suggesting might drive cell abnormalities COVID-19.
Introduction While complement is a contributor to disease severity in severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infections, all three pathways might be activated by the virus. Lectin pathway activation occurs through different pattern recognition molecules, including mannan binding lectin (MBL), protein shown interact with SARS-CoV-2 proteins. However, exact role of and its key molecule MBL COVID-19 still not fully understood. Methods We therefore investigated two...
ABSTRACT Background Autosomal dominant polycystic kidney disease (ADPKD) is the most common inherited disorder, characterized by development and enlargement of cysts, eventually leading to end-stage (ESKD). Pathogenic variants in PKD1 PKD2 genes are major cause ADPKD; additional rare GANAB, DNAJB11, ALG5 ALG9 have been found a minority ADPKD patients. More recently, significant number families linked monoallelic IFT140 gene. Methods In this retrospective study, we tested prevalence known...
Vulnerable subjects, including systemic lupus erythematosus (SLE) patients, have been prioritised to receive anti-SARS-CoV-2 vaccines. Few data about the safety of these vaccines in SLE are available. The aim our study is investigate SLE. We included 452 referring seven tertiary centres, who were immunised. A total 119 (26%) reported side effects (SE) after first and/or second shot (the most frequent SE fever, local reaction, fatigue, and arthralgia). Patients with constitutional symptoms...
Abstract Objectives Rituximab has become the cornerstone of induction treatment in ANCA-associated vasculitis (AAV). B-cell depletion may increase risk hypogammaglobulinemia, potentially leading to severe infections. This study aims assess factors associated with hypogammaglobulinemia AAV patients treated rituximab. Methods retrospective cohort included rituximab 14 European centres. Severe adverse events (SAEs) were defined as episodes requiring hospitalization or intravenous antibiotics,...
Primary focal segmental glomerular sclerosis (FSGS) is a rare, likely immune-mediated disease. Rituximab (RTX) may play role in management, although data adults are scanty.We collected cases of RTX-treated primary FSGS within the Italian Society Nephrology Immunopathology Working Group and explored response rate (24-hour proteinuria <3.5 g <50% compared with baseline, stable estimated filtration rate).A total 31 patients were followed for at least 12 months; further follow-up (median 17...
Certain serum proteins, including C-reactive protein (CRP) and D-dimer, have prognostic value in patients with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Nonetheless, these factors are non-specific, providing limited mechanistic insight into the peripheral blood mononuclear cell (PBMC) populations that drive pathogenesis of COVID-19. To identify cellular phenotypes associated disease, we performed a comprehensive, unbiased analysis total plasma-membrane PBMC proteomes from...
Abstract Background Tolvaptan is the only disease-modifying treatment approved to slow kidney disease progression in ADPKD. Data on ADPKD outside of clinical trials are limited and usually deal with short-term observations characterized by a drop-out rate 21-56%. Methods In this retrospective, observational, multicenter study enrolling 122 Italian patients rapid progressive renal disease, we evaluated tolerability side effects real-life setting. A personalized strategy, based an accurate...