A5022827897- Complement system in diseases
- Coagulation, Bradykinin, Polyphosphates, and Angioedema
- Blood Coagulation and Thrombosis Mechanisms
- Drug Transport and Resistance Mechanisms
- COVID-19 Clinical Research Studies
- Mast cells and histamine
- SARS-CoV-2 and COVID-19 Research
- Platelet Disorders and Treatments
- Immune Cell Function and Interaction
- T-cell and B-cell Immunology
- Cell Adhesion Molecules Research
- Radiopharmaceutical Chemistry and Applications
- Monoclonal and Polyclonal Antibodies Research
- Urticaria and Related Conditions
- Vitamin K Research Studies
- Heat shock proteins research
- Computational Drug Discovery Methods
- Glycosylation and Glycoproteins Research
- Metal complexes synthesis and properties
- Pregnancy and preeclampsia studies
- Autoimmune Bullous Skin Diseases
- Birth, Development, and Health
- Angiogenesis and VEGF in Cancer
- Nanoparticle-Based Drug Delivery
- Hemophilia Treatment and Research
Semmelweis University
2016-2025
Hungarian Academy of Sciences
2001-2018
ImmunoGenes (Hungary)
2011
Rigshospitalet
2007
Eötvös Loránd University
1996-2007
Magyar Agrár- és Élettudományi Egyetem
2007
Lund University
2000
Karolinska University Hospital
2000
University of Siena
2000
Karolinska Institutet
1999-2000
The complement system is the other major proteolytic cascade in blood of vertebrates besides coagulation–fibrinolytic system. Among three main activation routes complement, lectin pathway (LP) has been discovered latest, and it still subject intense research. Mannose-binding (MBL), collectins, ficolins are collectively termed as pattern recognition molecules (PRMs) LP, they responsible for targeting LP to molecular patterns, e.g., on bacteria. MBL-associated serine proteases (MASPs)...
Abstract Endothelial cells play a critical role in inflammation by responding to several endogenous and exogenous proinflammatory stimuli. The three most studied factors that provide inflammatory signals endothelial are lipopolysaccharide (LPS), tumor necrosis factor (TNF)‐α, interleukin (IL)‐1β; however, their effects on were thoroughly compared at the level of gene expression only. Therefore, our aim was assess differences signaling pathways, adhesion molecules, cytokines induced human...
Objectives Uncontrolled thromboinflammation plays an important role in the pathogenesis of coronavirus disease (COVID-19) caused by SARS-CoV-2 virus. Complement was implicated as key contributor to this process, therefore we hypothesized that markers complement profile, indicative for activation state system, may be related severity and mortality COVID-19. Methods In prospective cohort study samples 102 hospitalized 26 outpatients with PCR-confirmed COVID-19 were analyzed. Primary outcome...
Vaccinations against SARS-CoV-2 reduce the risk of developing serious COVID-19 disease. Monitoring spike-specific IgG subclass levels after vaccinations may provide additional information on specific humoral immune response. Here, we examined presence and antibody subclasses in health-care coworkers vaccinated with vector- (Sputnik, AstraZeneca) or mRNA-based (Pfizer-BioNTech, Moderna) vaccines unvaccinated patients. We found that vector-based elicited lower total than mRNA vaccines. The...
Activation of the complement system can induce and enhance inflammatory reaction. Mannose-binding lectin-associated serine protease-1 (MASP-1) is an abundant protease lectin pathway; however, its physiological function unclear. In this study, we demonstrate for first time that MASP-1 able to activate Ca(2+) signaling, NF-kappaB, p38 MAPK pathways in cultured HUVECs. was initiated by only; related protease, MASP-2, had no such effect. The phenomenon dependent on proteolytic activity MASP-1,...
Several studies have investigated the interactions between C-reactive protein (CRP) and various complement proteins but none of them took into consideration different structural forms CRP. The aim our study was to investigate whether antigenic CRP are able bind C1q, trigger activation C1 complex ability factor H (FH) C4b-binding (C4BP). Interactions were analysed in enzyme-linked immunosorbent assay surface plasmon resonance tests followed a reconstituted system using purified C1r C1s...
It has been previously reported that serum levels of 70-kDa heat-shock protein (Hsp70) are elevated in preeclampsia. The aim the present study was to examine whether increased Hsp70 related clinical characteristics and standard laboratory parameters preeclamptic patients, as well markers inflammation (C-reactive protein), endothelial activation (von Willebrand factor antigen) or injury (fibronectin), trophoblast debris (cell-free fetal DNA) oxidative stress (malondialdehyde). Sixty-seven...
The activity of ADAMTS13, the von Willebrand factor (VWF) cleaving protease is low in several conditions, including HELLP (haemolysis, elevated liver enzymes, and platelet count) syndrome. As syndrome develops most cases on basis preeclampsia, our aim was to determine whether plasma ADAMTS13 decreased preeclampsia. Sixty-seven preeclamptic patients, 70 healthy pregnant women 59 non-pregnant were involved this case-control study. Plasma determined with FRETS-VWF73 assay, while VWF antigen...
C1-inhibitor (C1-INH) is an important regulator of the complement, coagulation, fibrinolytic and contact systems. The quantity protease/C1-INH complexes in blood proportional to level vivo activation these four cascade-like plasma enzyme Parallel determination C1-INH-containing could be understand regulatory role C1-INH diseases such as hereditary angioedema (HAE) due deficiency (C1-INH-HAE). We developed in-house ELISAs measure concentration formed with active proteases: C1r, C1s, MASP-1,...
Introduction While complement is a contributor to disease severity in severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infections, all three pathways might be activated by the virus. Lectin pathway activation occurs through different pattern recognition molecules, including mannan binding lectin (MBL), protein shown interact with SARS-CoV-2 proteins. However, exact role of and its key molecule MBL COVID-19 still not fully understood. Methods We therefore investigated two...
Microbial infection urges prompt intervention by the immune system. The complement cascade and neutrophil granulocytes are predominant contributors to this immediate anti-microbial action. We have previously shown that mannan-binding lectin-associated serine protease-1 (MASP-1), most abundant enzyme of lectin pathway, can induce p38-MAPK activation, NFkappaB signaling, Ca2+-mobilization in endothelial cells. Since chemotaxis transmigration depends on cell we aimed explore whether recombinant...
Functionalized nanoparticles (NPs) can penetrate into living cells and vesicles, opening up an extensive range of novel directions. For example, NPs are intensively employed in targeted drug delivery biomedical imaging. However, the real-time kinetics dynamics NP–living cell interactions remained uncovered. In this study, we situ monitored cellular uptake gold NPs—functionalized with positively charged alkaline thiol—into surface-adhered cancer cells, by using a high-throughput label-free...
Despite significant progress, resistance to chemotherapy is still the main reason why cancer remains a deadly disease. An attractive strategy target collateral sensitivity of otherwise multidrug resistant (MDR) cancer. In this study, our aim was catalog various compounds that were reported elicit increased toxicity in P-glycoprotein (Pgp)-overexpressing MDR cells. We show activity most serendipitously identified cells fact cell-line specific, and not influenced significantly by function Pgp....
Endothelial and complement activation were both associated with immunothrombosis, a key determinant of COVID-19 severity, but their interrelation has not yet been investigated. We aimed to determine von Willebrand factor (VWF) antigen (VWF:Ag) concentration, VWF collagen binding activity (VWF:CBA), disintegrin metalloproteinase thrombospondin type 1 motif, member 13 (ADAMTS13) (ADAMTS13:Ac), ratios in hospitalized patients, investigate how these parameters constellation relate disease...
IgG has the longest survival time in circulation of Ig classes and lowest fractional catabolic rate. The neonatal Fc receptor (FcRn) plays an important role regulating these processes. Recently, we have cloned bovine (bFcRn) alpha chain detected its expression various epithelial cells which are mediating secretion. However, function homeostasis not been investigated. In current study, analyzed binding affinity human IgGs to bFcRn using surface plasmon resonance by vitro radioreceptor assays....
The aim of our study was to examine whether increased circulating total cell-free DNA levels are related the clinical characteristics and standard laboratory parameters preeclamptic patients, markers inflammation, endothelial activation or injury, oxidative stress fetal levels.Circulating measured by real-time quantitative PCR in plasma samples obtained from 67 70 normotensive pregnant women. Standard parameters, C-reactive protein, von Willebrand factor antigen, fibronectin, malondialdehyde...
Tesmilifene, a tamoxifen analog with antihistamine action, has chemopotentiating properties in experimental and clinical cancer studies. In our previous works, tesmilifene increased the permeability of blood-brain barrier (BBB) animal culture models. Our aim was to investigate effects on brain microvessel rat RG2 glioma model reveal its mode action endothelial cells. Tesmilifene significantly fluorescein extravasation glioma. Short-term treatment reduced resistance for marker molecules...