Carla López-Causapé
A5085286118- Antibiotic Resistance in Bacteria
- Bacterial biofilms and quorum sensing
- Antibiotics Pharmacokinetics and Efficacy
- Cystic Fibrosis Research Advances
- Antibiotic Use and Resistance
- Pneumonia and Respiratory Infections
- Clostridium difficile and Clostridium perfringens research
- Bacterial Identification and Susceptibility Testing
- SARS-CoV-2 and COVID-19 Research
- Respiratory viral infections research
- Influenza Virus Research Studies
- Viral gastroenteritis research and epidemiology
- Plant Pathogenic Bacteria Studies
- SARS-CoV-2 detection and testing
- Evolution and Genetic Dynamics
- Vibrio bacteria research studies
- Bacteriophages and microbial interactions
- Pharmaceutical and Antibiotic Environmental Impacts
- Cardiac, Anesthesia and Surgical Outcomes
- Bacterial Genetics and Biotechnology
- Asthma and respiratory diseases
- Gut microbiota and health
- Antimicrobial Resistance in Staphylococcus
- Helicobacter pylori-related gastroenterology studies
- Microscopic Colitis
Hospital Universitario Son Espases
2016-2025
Instituto de Salud Carlos III
2017-2025
Health Research Institute of the Balearic Islands
2017-2025
Centro de Investigación Biomédica en Red
2022-2025
Red Espanola de Investigacion en Patologia Infecciosa
2014-2021
Institut de Virologie
2021
Sapienza University of Rome
2014
Pseudomonas aeruginosa healthcare-associated infections are one of the top antimicrobial resistance threats world-wide. In order to analyze current trends, we performed a Spanish nation-wide high-resolution analysis susceptibility profiles, genomic epidemiology and resistome P. over five-year time lapse.A total 3.180 nonduplicated clinical isolates from two surveys in October 2017 2022 were analyzed. MICs 13 antipseudomonals determined by ISO-EUCAST. Multidrug (MDR)/extensively drug...
This study assessed the molecular epidemiology, resistance mechanisms, and susceptibility profiles of a collection 150 extensively drug-resistant (XDR) Pseudomonas aeruginosa clinical isolates obtained from 2015 Spanish multicenter study, with particular focus on resistome analysis in relation to ceftolozane-tazobactam susceptibility. Broth microdilution MICs revealed that nearly all (>95%) were nonsusceptible piperacillin-tazobactam, ceftazidime, cefepime, aztreonam, imipenem, meropenem,...
Emergence of epidemic clones and antibiotic resistance development compromises the management Pseudomonas aeruginosa cystic fibrosis (CF) chronic respiratory infections. Whole genome sequencing (WGS) was used to decipher phylogeny, interpatient dissemination, WGS mutator genotypes (mutome) resistome a widespread clone (CC274), in isolates from two highly-distant countries, Australia Spain, covering an 18-year period. The coexistence divergent CC274 clonal lineages revealed, but without...
To undertake a Spanish nationwide survey on Pseudomonas aeruginosa molecular epidemiology and antimicrobial resistance. Up to 30 consecutive healthcare-associated P. isolates collected in 2017 from each of 51 hospitals were studied. MICs 13 antipseudomonal agents determined by broth microdilution. Horizontally acquired β-lactamases detected phenotypic methods PCR. Clonal was evaluated through PFGE MLST; at least one XDR isolate clone hospital (n = 185) sequenced. The most active...
Whole-genome sequencing (WGS) was used for the characterization of frequently extensively drug resistant (XDR) Pseudomonas aeruginosa sequence type 175 (ST175) high-risk clone. A total 18 ST175 isolates recovered from 8 different Spanish hospitals were analyzed; 4 French included comparison. The typical resistance profile penicillins, cephalosporins, monobactams, carbapenems, aminoglycosides, and fluoroquinolones. In phylogenetic analysis, four clustered together with two one regions....
Abstract It is well established that antibiotic treatment selects for resistance, but the dynamics of this process during infections are poorly understood. Here we map responses Pseudomonas aeruginosa to in high definition a lung infection single ICU patient. Host immunity and therapy with meropenem suppressed P. , second wave emerged due growth oprD wbpM resistant mutants evolved situ. Selection then led loss resistance by decreasing prevalence low fitness mutants, increasing frequency...
Abstract Objectives To determine the susceptibility profiles and resistome of Pseudomonas aeruginosa isolates from European ICUs during a prospective cohort study (ASPIRE-ICU). Methods 723 respiratory samples or perianal swabs 402 patients 29 sites in 11 countries were studied. MICs 12 antibiotics determined by broth microdilution. Horizontally acquired β-lactamases analysed through phenotypic genetic assays. The first 105 providing such WGS, including analysis previously defined genotypic...
Pseudomonas aeruginosa, a ubiquitous opportunistic pathogen considered one of the paradigms antimicrobial resistance, is among main causes hospital-acquired and chronic infections associated with significant morbidity mortality. This growing threat results from extraordinary capacity P. aeruginosa to develop resistance through chromosomal mutations, increasing prevalence transferable determinants (such as carbapenemases extended-spectrum β-lactamases), global expansion epidemic lineages. The...
Abstract Antibiotic resistance poses a global health threat, but the within-host drivers of remain poorly understood. Pathogen populations are often assumed to be clonal within hosts, and is thought emerge due selection for de novo variants. Here we show that mixed strain common in opportunistic pathogen P. aeruginosa . Crucially, evolves rapidly patients colonized by multiple strains through pre-existing resistant strains. In contrast, sporadically single novel mutations. However, strong...
Hypermutable Pseudomonas aeruginosa isolates (hypermutators) have been identified in patients with cystic fibrosis (CF) and are associated reduced lung function. Hypermutators display a greatly increased mutation rate an enhanced ability to become resistant antibiotics during treatment.
Chronic respiratory infection by Pseudomonas aeruginosa is a major cause of mortality in cystic fibrosis (CF). We investigated the interplay between three key microbiological aspects these infections: occurrence transmissible and persistent strains, emergence variants with enhanced mutation rates (mutators) evolution antibiotic resistance. For this purpose, 10 sequential isolates, covering up to an 8-year period, from each CF patients were studied. As anticipated, resistance significantly...
Imipenem and imipenem-relebactam MICs were determined for 1,445 Pseudomonas aeruginosa clinical isolates a large panel of isogenic mutants showing the most relevant mutation-driven β-lactam resistance mechanisms. Imipenem-relebactam showed highest susceptibility rate (97.3%), followed by colistin ceftolozane-tazobactam (both 94.6%).
Background The electronic nose (e-nose) detects volatile organic compounds (VOCs) in exhaled air. We hypothesized that the VOCs print is different stable vs. exacerbated patients with chronic obstructive pulmonary disease (COPD), particularly if latter associated airway bacterial infection, and e-nose can distinguish them. Methods Smell-prints of bacteria most commonly involved exacerbations COPD (ECOPD) were identified vitro. Subsequently, we tested our hypothesis 93 ECOPD, 19 them...
A ceftolozane-tazobactam- and ceftazime-avibactam-resistant Pseudomonas aeruginosa isolate was recovered after treatment (including azithromycin, meropenem, ceftolozane-tazobactam) from a patient that had developed ventilator-associated pneumonia COVID-19 infection. Whole-genome sequencing revealed the strain, belonging to ST274, acquired nonsense mutation leading truncated carbapenem porin OprD (W277X), 7-bp deletion (nt213Δ7) in NfxB (negative regulator of efflux pump MexCD-OprJ), two...
Inhaled administration of high doses aminoglycosides is a key maintenance treatment Pseudomonas aeruginosa chronic respiratory infections in cystic fibrosis (CF). We analyzed the dynamics and mechanisms stepwise high-level tobramycin resistance development vitro compared results with those isogenic pairs susceptible resistant clinical isolates. Resistance correlated fusA1 mutations vivo. pmrB mutations, conferring polymyxin resistance, were also frequently selected In contrast, mutational...
The increasing prevalence of nosocomial infections produced by multidrug-resistant (MDR) or extensively drug-resistant (XDR) Pseudomonas aeruginosa is frequently linked to widespread international strains designated high-risk clones. In this work, we attempted decipher the interplay between resistance profiles, clones, and virulence, testing a large (n = 140) collection well-characterized P. isolates from different sources (bloodstream infections, outbreaks, cystic fibrosis, environment) in...
Ceftazidime-avibactam (CZA) has emerged as a promising solution to the lack of new antibiotics against Pseudomonas aeruginosa infections. Data from in vitro assays CZA combinations, however, are scarce. The objective our study was perform time-kill analysis effectiveness alone and combination with other collection extensively drug-resistant (XDR) P. isolates. Twenty-one previously characterized representative XDR isolates were selected. Antibiotic susceptibility tested by broth...