Laura Viñuela
A5068923349- SARS-CoV-2 and COVID-19 Research
- HIV Research and Treatment
- HIV/AIDS drug development and treatment
- COVID-19 Clinical Research Studies
- SARS-CoV-2 detection and testing
- Antibiotic Resistance in Bacteria
- Pneumocystis jirovecii pneumonia detection and treatment
- Herpesvirus Infections and Treatments
- HIV/AIDS Research and Interventions
- Poxvirus research and outbreaks
- Reproductive tract infections research
- Bacterial biofilms and quorum sensing
- Cervical Cancer and HPV Research
- Antibiotic Use and Resistance
- Bacillus and Francisella bacterial research
- Fungal Biology and Applications
- Data Mining Algorithms and Applications
- Parvovirus B19 Infection Studies
- Syphilis Diagnosis and Treatment
- Viral gastroenteritis research and epidemiology
- Nosocomial Infections in ICU
- Urinary Tract Infections Management
- Algorithms and Data Compression
- Pancreatic function and diabetes
- Antifungal resistance and susceptibility
Instituto de Investigación Biosanitaria de Granada
2022-2024
Instituto de Salud Carlos III
2022-2024
Centro de Investigación Biomédica en Red
2022-2024
Centro de Investigación en Red en Enfermedades Cardiovasculares
2023
Health Research Institute of the Balearic Islands
2019
To undertake a Spanish nationwide survey on Pseudomonas aeruginosa molecular epidemiology and antimicrobial resistance. Up to 30 consecutive healthcare-associated P. isolates collected in 2017 from each of 51 hospitals were studied. MICs 13 antipseudomonal agents determined by broth microdilution. Horizontally acquired β-lactamases detected phenotypic methods PCR. Clonal was evaluated through PFGE MLST; at least one XDR isolate clone hospital (n = 185) sequenced. The most active...
Imipenem and imipenem-relebactam MICs were determined for 1,445 Pseudomonas aeruginosa clinical isolates a large panel of isogenic mutants showing the most relevant mutation-driven β-lactam resistance mechanisms. Imipenem-relebactam showed highest susceptibility rate (97.3%), followed by colistin ceftolozane-tazobactam (both 94.6%).
Abstract Background We evaluated the prevalence of transmitted drug resistance (TDR) to integrase strand-transfer inhibitors (INSTIs) and nucleoside/nucleotide reverse transcriptase (NRTIs) clinically relevant (CRR) in newly diagnosed people with human immunodeficiency virus (HIV; PWH) naive antiretroviral therapy (ART) Europe. Methods MeditRes is a consortium that includes ART-naive PWH France, Greece, Italy, Portugal, Spain during 2018–2021. Reverse INSTI sequences were provided by...
Abstract Objectives To assess the effectiveness and tolerability of dolutegravir (DTG)/lamivudine (3TC) among treatment-naive virologically suppressed treatment-experienced individuals in multicentre cohort Spanish HIV/AIDS Research Network (CoRIS) during years 2018–2021. Methods We used multivariable regression models to compare viral suppression (VS) [HIV RNA load (VL) <50 copies/mL] change CD4 cell counts at 24 48 (±12) weeks after initiation with dolutegravir/lamivudine or other...
Recombination is an evolutionary strategy to quickly acquire new viral properties inherited from the parental lineages. The systematic survey of SARS-CoV-2 genome sequences Andalusian genomic surveillance has allowed detection unexpectedly high number co-infections, which constitute ideal scenario for emergence recombinants. Whole sequence been carried out as part programme. Sample sources included main hospitals in Andalusia region. In addition increase co-infections and known recombinants,...
Abstract The aim of this study was to assess the immunogenicity severe acute respiratory syndrome coronavirus 2 (SARS‑CoV‑2) vaccines among people living with HIV (PLWH) immunosuppression, after a booster dose. design case–control nested in prospective cohort PLWH. All patients CD4 cell count <200 cells/mm 3 who had received additional dose messenger RNA (mRNA) COVID‐19 vaccine, standard immunization scheme were included. Control group: age‐ and sex‐matched, ≥ 200 , ratio 2:1. Antibody...
Abstract Objectives To evaluate the correlation of O-antigen serotypes with resistance profiles and high-risk clones in a Spanish nationwide survey. Methods Up to 30 consecutive healthcare-associated Pseudomonas aeruginosa isolates were collected during October 2017 from each 51 hospitals (covering all regions) total 1445 studied. MICs 13 antipseudomonal agents MDR/XDR had been previously determined, as well whole-genome sequences 185 representative XDR isolates. (O1–O16) determined by...
Integrase resistance has not been reported with co-formulated dolutegravir/lamivudine in clinical trials or real-life cohorts. We aim to report, the best of our knowledge, first case selection key integrase mutation R263K a subject treated this regimen started as switch strategy undetectable plasma HIV-1 viraemia.Clinical report.A patient long-term suppressed viraemia (<50 copies/mL) no known risk factors for virological failure and never exposed previously an inhibitor developed...
Limited data are available regarding the susceptibility of reverse transcriptase V106 polymorphism to doravirine.
Abstract Background and Aim Until the May 2022 Monkeypox (MPXV) outbreak, which spread rapidly to many non‐endemic countries, virus was considered a viral zoonosis limited some African countries. The Andalusian circuit of genomic surveillance applied characterize MPXV outbreak in South Spain. Methods Whole genome sequencing used obtain profiles samples collected across south Spain, representative all provinces Andalusia. Phylogenetic analysis study relationship isolates available sequences...
The mpox outbreak, caused by monkeypox virus (MPXV), accelerated the development of molecular diagnostics. In this study, we detail evaluation Research Use Only (RUO) NeuMoDx MPXV assay multiple European and US sites. was designed developed Qiagen for Molecular Systems. Primers probes were tested specificity inclusivity
Objectives: More than two years into the COVID-19 pandemic, SARS-CoV-2 still remains a global public health problem. Successive waves of infection have produced new variants with mutations for which impact on severity and patient survival is uncertain. Methods: A total 764 genomes, sequenced from patients, hospitalized 19th February 2020 to 30 April 2021, along their clinical data, were used analysis. Results: significant association B.1.1.7, alpha lineage, mortality (log hazard ratio (LHR)...
Abstract To evaluate the prevalence of transmitted drug resistance (TDR) to nucleoside and nonnucleoside reverse transcriptase inhibitors (NRTI, NNRTI), protease (PI), integrase strand transfer (INSTI) in Spain during period 2019–2021, as well clinically relevant (TCRR) antiretroviral drugs. Reverse (RT), (Pro), Integrase (IN) sequences from 1824 PLWH (people living with HIV) were studied. TDR we investigated surveillance mutations (SDRM). TCRR (any level ≥ 3), for HIV subtyping used...
Azole resistance screening in
Vaginitis is a prevalent clinical disorder associated with several adverse health consequences, prompting women to seek medical care. In this study we evaluate the Vaginal Panel Real-Time PCR kit (qPCR test) against routinely used diagnostics for detection of bacterial vaginosis (BV), vulvovaginal candidiasis (VVC), and trichomoniasis. A total 1011 vaginal swab specimens were analyzed. The diagnostic methods BV was Gram stain-based Nugent score. VVC presence detected by culture, Candida...
Abstract This study aimed to assess the ability of a real-time reverse transcription polymerase chain reaction (RT-PCR) with multiple targets detect SARS-CoV-2 and its variants in single test. Nasopharyngeal specimens were collected from patients Granada, Spain, between January 2021 December 2022. Five allele-specific RT-PCR kits used sequentially, each kit designed predominant variant at time. When Alpha was dominant, included HV69/70 deletion, E N genes. Delta replaced Alpha, incorporated...
Abstract After more than two years of COVID-19 pandemic, SARS-CoV-2 still remains a global public health problem. Successive waves infection have produced new variants with mutations whose impact on severity and patient survival is uncertain. A total 764 genomes sequenced from patients, hospitalized 19th February 2020 to 30st April 2021, along their clinical data, were used for analysis. significant association B.1.1.7, the alpha lineage, mortality (Log Hazard ratio LHR=0.51,...
The incorporation of genomic sequencing techniques through next-generation has revolutionized clinical microbiology, innovating and improving the diagnosis infectious diseases. Today, whole genome in diseases many applications virology, bacteriology, antibiotic resistance, epidemiology, public health. With appearance SARS-CoV-2, importance analysis study genetic sequences been underlined. Since initial identification to date, more than 414,575 complete have shared worldwide access databases....
Introduction Vaccine-induced immunity against COVID-19 generates antibody and lymphocyte responses. However, variability in titers has been observed after vaccination, the determinants of a better response should be studied. The main objective this investigation was to analyze inflammatory biomarker induced healthcare workers vaccinated with BNT162b2, its association anti-Spike (a SARS-CoV-2 antigen) antibodies measured throughout 1-year follow-up. Methods Anti-spike 92 biomarkers were...
Abstract Until the May 2022 Monkeypox outbreak, which spread rapidly to many non-endemic countries, virus was considered a viral zoonosis limited some African countries. The Andalusian circuit of genomic surveillance applied characterize outbreak in South Spain. Whole genome sequencing used obtain profiles samples collected across south Spain, representative all provinces Andalusia. Phylogenetic analysis study relationship isolates and available sequences outbreak. total 160 monkeypox...
Abstract Vaginitis is a common clinical disorder for which women seek medical care. A evaluation of the Vaginal Panel Realtime PCR kit (qPCR test) compared with reference methods was performed. total 1011 vaginal swab specimens were analyzed. The Gram strain-based Nugent score combined Gardnerella culture bacterial vaginosis (BV) diagnosis and Candida species Trichomonas vaginalis detection. identification performed using matrix-assisted laser desorption ionization-time flight mass...