A5100651968- Antibiotic Resistance in Bacteria
- Mycobacterium research and diagnosis
- Tuberculosis Research and Epidemiology
- Antibiotics Pharmacokinetics and Efficacy
- Bacterial Identification and Susceptibility Testing
- Pneumonia and Respiratory Infections
- Antibiotic Use and Resistance
- Salmonella and Campylobacter epidemiology
- COVID-19 Clinical Research Studies
- Gut microbiota and health
- SARS-CoV-2 and COVID-19 Research
- Nosocomial Infections in ICU
- Pneumocystis jirovecii pneumonia detection and treatment
- Clostridium difficile and Clostridium perfringens research
- Diagnosis and treatment of tuberculosis
- Spanish Literature and Culture Studies
- Respiratory viral infections research
- Hemoglobin structure and function
- Sleep and related disorders
- Helicobacter pylori-related gastroenterology studies
- Phenothiazines and Benzothiazines Synthesis and Activities
- HIV/AIDS Research and Interventions
- Antimicrobial Resistance in Staphylococcus
- HIV/AIDS drug development and treatment
- Liver Disease and Transplantation
Hospital General Universitario de Elche
2006-2025
Hospital Virgen de la Concha
2020-2025
Instituto de investigación sanitaria y biomédica de Alicante
2017-2025
Hospital General Universitario de Alicante Doctor Balmis
2016-2025
Universitat de Miguel Hernández d'Elx
2013-2025
University of Manitoba
2025
Marqués de Valdecilla University Hospital
2004-2024
Universidad de Cantabria
2024
Hospital General de Granollers
2024
Instituto de Investigación Marqués de Valdecilla
2024
We evaluated the Binax NOW rapid immunochromatographic membrane test (ICT) for detection of Streptococcus pneumoniae urinary antigen in a population-based prospective study adults with community-acquired pneumonia (CAP). ICT was performed urine samples obtained from 452 (91.7%) 493 patients enrolled. Pneumococcal detected 19 (70.4%) 27 pneumococcal pneumonia. The results were more frequently positive who had not received antibiotics before testing (26.6% vs. 12.1%; P = .002). Only 16 (10.3%)...
Background Whole genome sequencing provides better delineation of transmission clusters in Mycobacterium tuberculosis than traditional methods. However, its ability to reveal individual links within is limited. Here, we used a 2-step approach based on Bayesian reconstruction (1) identify likely index and missing cases, (2) determine risk factors associated with transmitters, (3) estimate when happened. Methods findings We developed our method using genomic epidemiological data from...
Background SARS-CoV-2 is an RNA virus causing COVID-19. The clinical characteristics and epidemiology of COVID-19 have been extensively investigated, however, only one study so far focused on the patient’s nasopharynx microbiota. In this we investigated microbial community patients that developed different severity levels We performed 16S ribosomal DNA sequencing from nasopharyngeal swab samples obtained positive (56) negative (18) in province Alicante (Spain) their first visit to hospital....
Background Estimating the global influenza burden in terms of hospitalization and death is important for optimizing prevention policies. Identifying risk factors mortality allows design strategies tailored to groups at highest risk. This study aims (a) describe clinical characteristics hospitalizations with a diagnosis over five flu seasons (2016–2017 2020–2021), (b) assess associated morbidity (hospitalization rates ICU admissions rate), cost different age (c) analyze mortality. Methods...
Abstract Background In the present study we analyze, in patients with breast cancer, tumor expression of androgen receptors (AR), its relationship clinicopathological characteristics and several matrix metalloproteases (MMPs) their inhibitors (TIMPs), as well prognosis. Methods An immunohistochemical was performed using tissue microarrays specific antibodies against AR, MMPs -1, -2, -7, -9, -11, -13, -14, TIMPs -2 -3. More than 2,800 determinations on specimens from 111 primary invasive...
<i>Objective:</i> Our aim was to study the influence of efflux pump systems in resistance <i>Mycobacterium tuberculosis</i> fluoroquinolones and linezolid. <i>Methods:</i> We studied mutations <i>gyrA</i> <i>gyrB</i> genes with 2 inhibitors (reserpine MC 207.110). <i>Results:</i> The effect active system on decrease sensitivity ciprofloxacin, moxifloxacin, levofloxacin, ofloxacin, gatifloxacin linezolid by investigating...
The purpose of the current work was to study pattern and dynamics biofilm formation in clinical isolates Staphylococcus aureus epidermidis presence 10 antibiotics with different action mechanisms. By using impedance measurements microtitre plates gold electrodes we have assessed antibiotic effect on bacterial growth real time. appear combine both cellular matrix production, representing a measurement total mass. Several reference strains were tested, showing slopes cell index values which...
Abstract Objectives To assess the effectiveness and tolerability of dolutegravir (DTG)/lamivudine (3TC) among treatment-naive virologically suppressed treatment-experienced individuals in multicentre cohort Spanish HIV/AIDS Research Network (CoRIS) during years 2018–2021. Methods We used multivariable regression models to compare viral suppression (VS) [HIV RNA load (VL) &lt;50 copies/mL] change CD4 cell counts at 24 48 (±12) weeks after initiation with dolutegravir/lamivudine or other...
BackgroundWhile a low CD4/CD8 ratio during HIV treatment correlates with immunosenescence, its value in identifying patients at an increased risk for clinical events remains unclear.MethodsWe analyzed data from the CoRIS cohort to determine whether CD4 count, CD8 and year two of antiretroviral therapy (ART) could predict serious non-AIDS (SNAEs) next five years. These included major adverse cardiovascular events, non-AIDS-defining malignancies, non-accidental deaths. We used pooled logistic...
Numbers of desquamated epithelial cells, yeast cells and bacterial organisms were counted in samples collected from the external ear canal 37 normal dogs 16 cats, 24 22 cats with otitis externa. The aims study to establish quantitative reference ranges correlate these data clinical status cats. significantly increased (P = 0.05; P 0.0001) 0.0001; externa, most cases high counts correlated signs. Mean Malassezia per high-power dry field > or 5 dog 12 cat considered abnormal. 25 15 When used...
The extreme limitation of free iron has driven various pathogens to acquire from the host in form heme. Specifically, several Gram-negative secrete a heme binding protein known as HasA scavenge extracellular environment and transfer it receptor HasR for import into bacterial cell. Structures heme-bound apo-HasA homologues show that iron(III) ligands, His32 Tyr75, reside on loops extending core significant conformational change must occur at loop upon binding. Here, we investigate kinetics...
When challenged by low-iron conditions several Gram-negative pathogens secrete a hemophore (HasA) to scavenge hemin from its host and deliver it receptor (HasR) on their outer membrane for internalization. Here we report results studies aimed at probing the structural dynamic processes play in loading of apo-hemophore secreted P. aeruginosa (apo-HasAp) with hemin. The structure apo-HasAp shows large conformational change loop harboring axial ligand His32 relative holo-HasAp, whereas bearing...
The aim of this study was to determine the diagnostic usefulness quantification H. pylori genome in detection infection patients with upper gastrointestinal bleeding (UGB). A total 158 consecutive digestive disorders, 80 whom had clinical presentation UGB, were studied. number microorganisms quantified using a real-time PCR system which amplifies urease gene an internal control for eliminating false negatives. biopsy sample from antrum and corpus each patient processed. rapid test, culture,...