A5028368012- Ovarian cancer diagnosis and treatment
- Protease and Inhibitor Mechanisms
- HER2/EGFR in Cancer Research
- Glycosylation and Glycoproteins Research
- Cell Adhesion Molecules Research
- Peptidase Inhibition and Analysis
- Colorectal Cancer Treatments and Studies
- Proteoglycans and glycosaminoglycans research
- Gastric Cancer Management and Outcomes
- Cancer-related gene regulation
- Epigenetics and DNA Methylation
- Genetic factors in colorectal cancer
- Breast Cancer Treatment Studies
- Hippo pathway signaling and YAP/TAZ
- Immune Cell Function and Interaction
- Cancer Cells and Metastasis
- Renal and related cancers
- Fibroblast Growth Factor Research
- Cancer-related Molecular Pathways
- Helicobacter pylori-related gastroenterology studies
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Brain Metastases and Treatment
- Lung Cancer Treatments and Mutations
- Genomics and Chromatin Dynamics
- Galectins and Cancer Biology
Instituto de Investigación Sanitaria del Principado de Asturias
2003-2025
Gobierno del Principado de Asturias
2003-2025
Central University Hospital of Asturias
2015-2024
Centro de Investigación del Cáncer
2023
Universidad de Salamanca
2023
Institut Jacques Monod
2023
Nanomaterials and Nanotechnology Research Center
2018
Universidad de Oviedo
2018
Fundación Hospital de Jove
2006-2009
Unidad de Investigación en cuidados y servicios de salud (Investén-isciii)
2007
An immunohistochemical study was performed using tissue microarrays and specific antibodies against matrix metalloproteinases (MMPs) 1, 2, 7, 9, 11, 13, 14, their tisullar inhibitors (TIMPs) 3. More than 2600 determinations on cancer specimens from 131 patients with primary ductal invasive tumours of the breast (65 66 without distant metastasis) controls were performed. Staining results categorised a score based intensity staining software program calculated percentage immunostained cells...
Abstract Background In the present study we analyze, in patients with breast cancer, tumor expression of androgen receptors (AR), its relationship clinicopathological characteristics and several matrix metalloproteases (MMPs) their inhibitors (TIMPs), as well prognosis. Methods An immunohistochemical was performed using tissue microarrays specific antibodies against AR, MMPs -1, -2, -7, -9, -11, -13, -14, TIMPs -2 -3. More than 2,800 determinations on specimens from 111 primary invasive...
Loss of 5-hydroxymethylcytosine (5hmC) has been associated with mutations the ten-eleven translocation (TET) enzymes in several types cancer. However, tumors wild-type TET genes can also display low 5hmC levels, suggesting that other mechanisms involved gene regulation might be implicated decline this epigenetic mark. Here we show DNA hypermethylation and loss hydroxymethylation, as well a marked reduction activating histone marks TET3 gene, impair expression lead to genome-wide levels...
An immunohistochemical study was performed using tissue microarrays and specific antibodies against matrix metalloproteinase (MMP)-1, -2, -7, -9, -11, -13 –14, tissular inhibitors of (TIMP)-1, -2 -3. More than 2600 determinations on cancer specimens from 131 patients with primary ductal invasive tumours the breast were performed. To identify groups distinct expression profiles data analysed by unsupervised hierarchical cluster analysis each cellular type. We did not find well-defined cases...
Abstract Previous studies have suggested a relationship between the number of CAG triplet repeats in HTT gene and neurodegenerative diseases not related to Huntington's disease (HD). This study seeks investigate whether is associated with risk developing certain tauopathies its influence as modulator clinical neuropathological phenotype. Additionally, it aims evaluate potential polyglutamine staining screening. We genotyped repeat APOE‐ℰ isoforms cohort patients diagnoses ( n =588),...
// María G. García 1, 2 , Antonella Carella Rocío Urdinguio Gustavo F. Bayón 1 Virginia Lopez Juan Ramón Tejedor 3 Marta I. Sierra Estela García-Toraño Pablo Santamarina Raúl Perez Cristina Mangas Aurora Astudillo 4 M. Daniela Corte-Torres 5 Inés Sáenz-de-Santa-María 6 María-Dolores Chiara Agustín Fernández and Mario Fraga Institute of Oncology Asturias (IUOPA), HUCA, Universidad de Oviedo,...
Abstract Adult-type granulosa cell tumors (AGCT) are the most common type of malignant ovarian sex cord–stromal tumors. Most AGCTs carry somatic variant c.402C>G (p.C134W) affecting transcription factor FOXL2. Germline dominant variants in FOXL2 responsible for blepharophimosis syndrome, which is characterized by underdevelopment eyelid. In this work, we generated a mouse model harboring C134W to evaluate vivo poorly understood oncogenic role The mutation was regarding eyelid...
Abstract Background Late-onset Alzheimer’s disease (LOAD) accounts for more than 95% of AD cases. Previously, we have described that 6% patients present CAG intermediate alleles in the huntingtin gene ( HTT IA s). The caudate nucleus, most affected region Huntington’s disease, is highly sensitive to these expansions, as they can induce epigenetic changes, including altered microRNA profiles. All this implies a potential source expression deregulation, affecting onset and/or progression LOAD...
Phosphoinositide 3-kinase (PI3K) and PTEN-induced kinase 1 (PINK1) are key regulators of metabolism mitochondrial quality control. This study assessed their immunoexpression in 22 patients with lung adenocarcinoma resected brain metastases treated curative intent (2007–2017) evaluated prognostic significance. Tissue microarrays primary tumors matched were analyzed using the H-score method. PI3K expression was significantly higher than (96.8 ± 57.9 vs. 43.5 62.3; p = 0.003) stage IV...
Background: Guided bone regeneration (GBR) is a widely used technique in oral and maxillofacial surgery to restore lost bone. The aim of this study evaluate the effectiveness titanium occlusive barriers GBR for increasing volume both vertical horizontal dimensions. Methods: A prospective analysis was conducted on 11 patients (15 cases) undergoing augmentation with combined graft biomaterials dental implant placement. Bone gain assessed using pre- postoperative low-dose cone beam computed...
Phosphoinositide 3-kinase (PI3K) and PTEN-induced kinase 1 (PINK1) are key regulators of metabolism mitochondrial quality control. This study assessed their immunoexpression in 22 patients with lung adenocarcinoma resected brain metastases who underwent curative treatment between 2007 2017 evaluated prognostic significance. Tissue microarrays primary tumors matched were analyzed using the H-score method. PI3K expression was significantly higher (96.8 ± 57.9 vs. 43.5 62.3; p = 0.003) stage IV...
p53 wild-type is a tumor suppressor gene involved in DNA transcription or repair mechanisms. When damage to unrepairable, induces programmed cell death (apoptosis). The mutant the most frequent molecular alteration human cancer, including breast cancer. Here, we analyzed genetic alterations oncogene expression 55 patients with cancer at different stages and 8 normal women. We measured by ELISA assay serum levels of protein antibodies. Immunohistochemistry RT-PCR using specific primers as...
Background Matrix metalloproteases (MMPs), enzymes with the ability to degrade extracellular matrix, play an important role in tissue invasion by cutaneous malignant melanoma (CMM). One specific MMP, collagenase-3 (MMP-13), is thought have a key function activation of MMP. Aims To evaluate expression MMP-13 CMM and assess its possible relationship clinical pathological parameters. Methods was analyzed 51 paraffin-embedded tumor samples from patients invasive CMM, ten situ melanomas, eight...
Cathepsin D is the proteolytic enzyme most frequently implicated as a prognostic factor in primary breast cancer. In present study we evaluated by means of an immunoradiometric assay tumor content this protease cancer, its relationship with tumor-related clinical and pathological parameters, significance large series cancer patients.The comprised 1033 women histologically established invasive was measured cytosol samples to determine total amount cathepsin (52 kDa, 48 kDa 34 kDa). Evaluation...
Background: CD44s (standard isoform) is a cell adhesion molecule belonging to the family of hyaluronan-binding proteins. The CD44 has been found be overexpressed in epithelial tumors, where they are generally relationship with tumor growth and metastasic properties. aim this work was evaluate membranous content colorectal cancer healthy surrounding mucosa, its possible clinicopathological parameters, potential prognostic significance. Materials Methods: Membranous levels were measured by an...
Objective. To determine whether the expression of collagenase-3 (MMP-13) in biopsies from patients with inflammatory bowel disease is correlated histological inflammation parameters. Material and methods. Fifty-nine were included study. The control group comprised 20 free ten acute diverticulitis. MMP-13 was determined by immunohistochemical staining specimens assigned a score. Results. It found that 62.8% ulcerative colitis (UC) 54.1% Crohn's (CD) showed MMP-13-positive immunostaining...
Aims: To analyse the expression of metalloproteinases (MMPs) and their inhibitors (TIMPs) in ductal carcinoma situ breast (DCIS). Methods results: An immunohistochemical study was performed 56 patients with pure DCIS, 39 DCIS adjacent to invasive (IDC) 63 T1 IDC, using tissue microarrays specific antibodies against MMPs TIMPs. Immunohistochemical results were categorized a software program. The data analysed by unsupervised hierarchical cluster analysis each cellular type. IDC showed higher...
Previous studies suggest a link between CAG repeat number in the HTT gene and non-Huntington neurodegenerative diseases.The aim is to analyze whether expanded alleles and/or their size are associated with risk for developing α-synucleinopathies or behavior as modulators of phenotype.We genotyped APOE-Ɛ isoforms case-control series including patients either clinical neuropathological diagnosis α-synucleinopathy.We identified three Parkinson's disease (PD) (0.30%) two healthy controls (0.19%)...