A5007859367- Glioma Diagnosis and Treatment
- Epigenetics and DNA Methylation
- Meningioma and schwannoma management
- Neurofibromatosis and Schwannoma Cases
- Neuroblastoma Research and Treatments
- Histone Deacetylase Inhibitors Research
- Hedgehog Signaling Pathway Studies
- Radiomics and Machine Learning in Medical Imaging
- Genomics and Chromatin Dynamics
- Cancer Genomics and Diagnostics
- RNA Research and Splicing
- Cancer-related Molecular Pathways
- Cancer, Hypoxia, and Metabolism
- Lymphoma Diagnosis and Treatment
- Chromatin Remodeling and Cancer
- Genomic variations and chromosomal abnormalities
- Sarcoma Diagnosis and Treatment
- MRI in cancer diagnosis
- Renal cell carcinoma treatment
- Vascular Malformations Diagnosis and Treatment
- Microtubule and mitosis dynamics
- Protein Degradation and Inhibitors
- RNA modifications and cancer
- Cancer-related molecular mechanisms research
- Gene expression and cancer classification
Complejo Hospitalario Universitario de Toledo
2022-2025
Hospital Universitario de Toledo
2022-2025
Complejo Hospitalario de Cáceres
2024
Servicio de Salud de Castilla La Mancha
2023-2024
Hospital Universitario 12 De Octubre
2024
Erasmus Hospital
2024
Hospital Virgen de la Salud
2013-2023
Université Libre de Bruxelles
2018-2020
Jacobi Medical Center
2019
Hospital La Paz Institute for Health Research
2018
Purpose To evaluate the prognostic and predictive value of surface-derived imaging biomarkers obtained from contrast material–enhanced volumetric T1-weighted pretreatment magnetic resonance (MR) sequences in patients with glioblastoma multiforme. Materials Methods A discovery cohort five local institutions (165 patients; mean age, 62 years ± 12 [standard deviation]; 43% women 57% men) an independent validation (51 60 12; 39% 61% The Cancer Imaging Archive contrast-enhanced MR were included...
We explored the rationale for treating glioblastoma (GBM) with regorafenib. In 103 newly diagnosed GBM patients, we assessed mutations, copy number variants (CNVs), fusions, and overexpression in 46 genes encoding protein kinases (PKs) potentially targeted by regorafenib or its metabolites performed a functional enrichment analysis to assess their implications angiogenesis. analyzed regorafenib’s binding inhibitory activity target affinity these PKs focused on subset of 18 inhibited at...
Conventional cytogenetic and comparative genomic hybridization (CGH) studies in brain malignancies have shown that glioblastoma multiforme (GBM) is characterized by complex structural numerical alterations. However, the limited resolution of these techniques has precluded precise identification detailed specific gene copy number alterations.We performed a genome-wide survey changes 20 primary GBMs CGH on cDNA microarrays. A novel amplicon at 4p15, previously uncharacterized amplicons...
Polo-like kinases (Plks) are characterized by the presence of a specific domain, known as polo box (PBD), involved in protein-protein interactions. Plk1 to Plk4 centrosome biology well regulation mitosis, cytokinesis, and cell cycle checkpoints response genotoxic stress. We have analyzed here new member vertebrate family, Plk5, protein that lacks kinase domain humans. Plk5 does not seem role progression; fact, it is downregulated proliferating cells accumulates quiescent cells. This mostly...
Loss of 5-hydroxymethylcytosine (5hmC) has been associated with mutations the ten-eleven translocation (TET) enzymes in several types cancer. However, tumors wild-type TET genes can also display low 5hmC levels, suggesting that other mechanisms involved gene regulation might be implicated decline this epigenetic mark. Here we show DNA hypermethylation and loss hydroxymethylation, as well a marked reduction activating histone marks TET3 gene, impair expression lead to genome-wide levels...
Abstract Purpose: T-Cell lymphomas constitute heterogeneous and aggressive tumors in which pathogenic alterations remain largely unknown. Expression profiling has demonstrated to be a useful tool for molecular classification of tumors. Experimental Design: Using DNA microarrays (CNIO-OncoChip) containing 6386 cancer-related genes, we established the expression T-cell compared them normal lymphocytes lymph nodes. Results: We found significant differences between peripheral lymphoblastic...
Primary glioblastoma multiforme (GBM), in contrast with secondary GBM, has been associated the presence of EGFR amplification and absence p53 mutation. In this study, we analyzed relevant molecular clinical variables 194 primary GBMs tested them for survival analysis. Although most tumors showed a mutually exclusive pattern, concurrent alterations were detected. Survival analysis CDK4 revealed highly significant association worse outcome (P = .01), whereas MDM2, CDK6, PTEN, p21 not patient...
Abstract A close relationship has been demonstrated between genomic complexity and clinical outcome in uterine smooth muscle tumors. We studied the profiles by array‐CGH of 28 fumarate hydratase deficient leiomyomas 37 with bizarre nuclei (LMBN) from 64 patients. Follow‐up was available for 46 patients (from three to 249 months, mean 87.3 months). All were alive without evidence disease. For 51 interpretable tumors Genomic Index (GI) 16.4 (median: 9.8; 1 57.8), significantly lower than GI...
Knowledge of the molecular mechanisms involved in biology glioblastoma multiforme (GBM) is essential for identification candidate prognostic markers, new putative therapeutic targets, and early detection strategies predictive survival.The authors performed expression-profiling analyses a series primary GBMs by using complementary DNA microarrays. Validation targets was large immunohistochemistry on tissue microarrays, real-time quantitative reverse transcription-polymerase chain reaction...
The majority of meningiomas are probably benign but a number tumors display considerable histological and/ or clinical aggressivity, sometimes with unexpectedly high recurrence rates after radical removal.Understanding the potential behavior these in individual patients is critical for rational therapeutic decision-making.This study aimed to identify gene expression profiles and candidate markers associated original recurrent meningiomas.Unsupervised hierarchical clustering samples confirmed...
Vestibular schwannomas are benign neoplasms that arise from the vestibular nerve. The hallmark of these tumors is biallelic inactivation neurofibromin 2 (NF2). Transcriptomic alterations, such as neuregulin 1 (Nrg1)/ErbB2 pathway, have been described in schwannomas. In this study, we performed a whole transcriptome analysis 31 and 9 control nerves Affymetrix Gene 1.0 ST platform, validated by quantitative real-time PCR (qRT-PCR) using TaqMan low density arrays. We mutational NF2...
Meningiomas are the most common primary brain tumors; they arise from coverings of brain. Although meningiomas generally benign, some more clinically aggressive, as reflected by their histopathological features or unexpected recurrence. We hypothesized that recurrent histologically benign might have genetic in with those showing a aggressive histology. By comparing gene expression profiles associated meningioma progression and recurrence 128 tumor samples (i.e. 83 World Health Organization...
According to World Health Organization criteria, diffuse gliomas are divided into several histological subtypes, including astrocytomas, oligodendrogliomas, and oligoastrocytomas, 4 malignancy grades (I-IV). Molecular alterations, such as the isocitrate dehydrogenase gene (IDH) mutation or 1p/19q loss, found in these tumors but not included current classification system. Recently, of α thalassemia/mental retardation syndrome X-linked (ATRX) its loss expression have been reported infiltrating...
Glioblastoma (GBM) is the most common malignant adult primary brain tumor. Despite its high lethality, a small proportion of patients have relatively long overall survival (OS). Here we report study series 74 GBM samples from 29 long-term survivors ([LTS] OS ≥36 months) and 45 non-LTS. Using next-generation sequencing, analyzed genetic alterations in genes frequently altered gliomas. Approximately 20% LTS had mutation IDH1 or IDH2 (IDH) genes, denoting relevance this molecular prognostic...
Giant cell glioblastoma (gcGBM) is a rare morphological variant of IDH-wildtype (IDHwt) GBM that occurs in young adults and have slightly better prognosis than "classic" IDHwt GBM.We studied 36 GBMs, 14 with histopathological diagnosis gcGBM 22 giant component. We analyzed the genetic profile most frequently mutated genes gliomas assessed tumor mutation load (TML) by gene-targeted next-generation sequencing. validated our findings using The Cancer Genome Atlas (TCGA) data.p53 was altered...
Background: Glioblastoma (GB) is a highly aggressive cancer with nearly 100% recurrence, even after removal of all contrast-enhancing (CE) disease. This primarily due to the persistence residual tumor cells in peritumoral brain zone (PBZ), which can repopulate GB treatment. The aim this study was characterize degree neoplastic infiltration and microenvironment (TME) periphery these tumors. Methods: We generated prospective collection radiologically guided biopsies from patients where we...
Abstract p73 is a candidate tumor suppressor and imprinted gene that shares significant homology with the p53 gene. It located on 1p36, region frequently deleted in neuroblastoma other tumors. To investigate pattern of inactivation this human lymphomas, we studied 59 tumors to identify abnormal methylation exon 1 loss heterozygosity (LOH) at locus. was methylated 13/50 (26%) B cell lymphomas. There no evidence 9 T lymphomas analyzed. Burkitt's showed highest proportion cases (36%), although...
Vestibular schwannomas are benign tumors that arise from Schwann cells in the VIII cranial pair and usually present NF2 gene mutations and/or loss of heterozygosity on chromosome 22q. Deregulation has also been found several genes, such as ERBB2 NRG1. MicroRNAs non-coding RNAs approximately 21 to 23 nucleotides length regulate mRNAs, by degradation at post-transcriptional level.We used microarray technology test deregulation miRNAs other GeneChip miRNA 1.0 (Affymetrix) over 16 vestibular 3...