An immunohistochemical study was performed using tissue microarrays and specific antibodies against matrix metalloproteinases (MMPs) 1, 2, 7, 9, 11, 13, 14, their tisullar inhibitors (TIMPs) 3. More than 2600 determinations on cancer specimens from 131 patients with primary ductal invasive tumours of the breast (65 66 without distant metastasis) controls were performed. Staining results categorised a score based intensity staining software program calculated percentage immunostained cells...

Toll-like receptors (TLRs) have garnered an extraordinary amount of interest in cancer research due to their role tumor progression. By activating the production several biological factors, TLRs induce type I interferons and other cytokines, which drive inflammatory response activate adaptive immune system. The aim this study was investigate expression clinical relevance TLR3, 4 9 breast cancer.The levels TLR4 TLR9 were analyzed on tumors from 74 patients with cancer. analysis performed by...

Aims Fibronectin ( FN ) has attracted interest in cancer research, owing to its role tumour progression. The aims of this study were investigate the expression and clinical relevance breast cancer, explore relationship with matrix metalloproteases MMP s) their inhibitors TIMP s). Methods results An immunohistochemical was performed using tumours from 110 patients, tissue arrays specific antibodies against , ‐7, ‐9, ‐11, ‐1, ‐2. indicated that related size, histological grade, ‐9 expression....

Abstract Background In the present study we analyze, in patients with breast cancer, tumor expression of androgen receptors (AR), its relationship clinicopathological characteristics and several matrix metalloproteases (MMPs) their inhibitors (TIMPs), as well prognosis. Methods An immunohistochemical was performed using tissue microarrays specific antibodies against AR, MMPs -1, -2, -7, -9, -11, -13, -14, TIMPs -2 -3. More than 2,800 determinations on specimens from 111 primary invasive...

Purpose/Objectives: To identify use of complementary and alternative medicine (CAM) for relief symptoms side effects among women diagnosed with breast cancer to demographic clinical factors associated the CAM in these patients.

An immunohistochemical study was performed using tissue microarrays and specific antibodies against matrix metalloproteinase (MMP)-1, -2, -7, -9, -11, -13 –14, tissular inhibitors of (TIMP)-1, -2 -3. More than 2600 determinations on cancer specimens from 131 patients with primary ductal invasive tumours the breast were performed. To identify groups distinct expression profiles data analysed by unsupervised hierarchical cluster analysis each cellular type. We did not find well-defined cases...

Extracellular matrix metalloproteases (MMPs) have raised an extraordinary interest in cancer research because of their potential role basal membrane and extracellular degradation, consequently facilitating tumour invasion metastases development. An immunohistochemical study was performed using tissue arrays specific antibodies against MMPs 1, 2, 7, 9, 11, 13, 14, inhibitors, TIMPs 2 3. More than 2600 determinations on specimens from 133 patients with clinically localised prostate carcinoma,...

Hepatocellular carcinoma (HCC) is in the 10 leading cancer types, being difficult to detect as most of patients who develop this tumor have no symptoms other than those related their long-standing liver disease. The constantly exposed bacterial products, viral infection, alcohol or which may be cause chronic damage, and thus an increasing risk for HCC. Toll-like receptors (TLR) gained extraordinary interest research due role several biological processes such innate immune responses,...

Tumors are infiltrated by macrophages, T and B-lymphocytes, which may favor tumor development promoting angiogenesis, growth invasion. The aim of this study was to investigate the clinical relevance relative amount macrophages (CD68+), T-cells (CD3+) B-cells (CD20+) at invasive front breast carcinomas, expression matrix metalloproteases (MMPs) their inhibitors (TIMPs) either or center. We performed an immunohistochemical counting CD3, CD20 CD68 positive cells front, in 102 carcinomas. Also,...

Inflammatory conditions may promote tumor progression and aggressiveness. In previous reports, we found a group of breast cancer tumors characterized by metalloprotease-11 (MMP-11) expression intratumoral mononuclear inflammatory cells (MICs), which was associated with distant metastasis development. Thus, in the present study evaluated relationship between MMP-11 MICs, development, wide panel factors carcinoma. an initial approach, analyzed 65 inflammation, population classified good or bad...

Abstract Background Triple-negative breast cancer (TNBC) is the most lethal subtype of and, despite its adverse effects, chemotherapy standard systemic treatment option for TNBC. Since, it utmost importance to consider combination different agents achieve greater efficacy and curability potential, MSC secretome a possible innovative alternative. Methods In present study, we proposed investigate anti-tumor effect chemical agent (paclitaxel) with complex biological product, derived from human...

The objective of the present work was to evaluate impact phenotype both mononuclear inflammatory cells (MICs) and cancer-associated fibroblast (CAFs) in early breast cancer patients, specifically assessed as their expression MMP/TIMP relative position within tumor (i.e., localization at center or invasive front) occurrence distant metastases.. An immunohistochemical study performed using tissue arrays specific antibodies against matrix metalloproteinase (MMP)−1, −2, −7, −9, −11, −13 −14,...

Toll-like receptors (TLRs) have raised an extraordinary interest in cancer research due to their role tumor progression. By activating the production of several biological factors, TLRs drive inflammatory response and activate adaptive immune system. The aim this study was investigate expression clinical relevance TLR3, TLR4, TLR9 gastric cancer. For purpose, immunohistochemical on specimens from 106 patients with performed using tissue arrays specific antibodies against TLR9. results...

Inflammatory bowel disease (IBD), including Crohn’s (CD) and ulcerative colitis (UC), is increasingly prevalent current therapies are not completely effective. Mesenchymal stem cells emerging as a promising therapeutic option. Here, the effect of local hydrogel application loaded with conditioned medium (CM) from human uterine cervical (hUCESC-CM) in an experimental acute mice model has been evaluated. Colitis induction was carried out C57BL/6 by dissolving dextran sulfate sodium (DSS)...

The Pit-1 transcription factor (also know as POU1F1) plays a critical role in cell differentiation during organogenesis of the anterior pituitary mammals and is transcriptional activator for gene transcription. Increased expression has been reported human tumorigenic breast cells. Here, we found that overexpression or knockdown cancer lines induced profound phenotypic changes proteins involved proliferation, apoptosis, invasion. Some these protumorigenic effects were mediated by upregulation...

Breast tumors infiltrated by matrix metalloprotease 11 (MMP-11)+ mononuclear inflammatory cells are prone to form metastases; express high levels of interleukin (IL)-1, IL-5, IL-6, IL-17, interferon β (IFNβ) and NFκB; exhibit an increased CD68+/(CD3+CD20+) cell ratio at their invasive front. These factors, which implicated in the crosstalk between microenvironment, may emerge as attractive prognostic factors therapeutic targets.