A5034274177- Antibiotic Resistance in Bacteria
- Bacterial biofilms and quorum sensing
- Infections and bacterial resistance
- Vibrio bacteria research studies
- Antibiotics Pharmacokinetics and Efficacy
- Antibiotic Use and Resistance
- Infectious Disease Case Reports and Treatments
- Spectroscopy Techniques in Biomedical and Chemical Research
- Bacterial Identification and Susceptibility Testing
- Pharmaceutical and Antibiotic Environmental Impacts
- Pneumonia and Respiratory Infections
- Mycobacterium research and diagnosis
- Genomics and Phylogenetic Studies
- Bacteriophages and microbial interactions
- Antimicrobial Resistance in Staphylococcus
- Escherichia coli research studies
- Brucella: diagnosis, epidemiology, treatment
- Bioactive Compounds and Antitumor Agents
- Microbial Metabolism and Applications
- Salmonella and Campylobacter epidemiology
- Aquatic Ecosystems and Phytoplankton Dynamics
- Plant Pathogenic Bacteria Studies
- Immune responses and vaccinations
- Enterobacteriaceae and Cronobacter Research
- Microbial Natural Products and Biosynthesis
Marqués de Valdecilla University Hospital
2015-2025
Instituto de Investigación Marqués de Valdecilla
2016-2025
Instituto de Salud Carlos III
2015-2025
Centro de Investigación Biomédica en Red
2023-2025
Red Espanola de Investigacion en Patologia Infecciosa
2015-2016
Fundación Marques de Valdecilla
2012-2014
Bicêtre Hospital
2012
Inserm
2012
Assistance Publique – Hôpitaux de Paris
2012
Université Paris-Sud
2012
Recent reports have revealed the existence of widespread extensively drug-resistant (XDR) P. aeruginosa high-risk clones in health care settings, but there is still scarce information on their specific chromosomal (mutational) and acquired resistance mechanisms. Up to 20 (10.5%) 190 bloodstream isolates collected from 10 Spanish hospitals met XDR criteria. A representative number (15 per group) classified as multidrug-resistant (MDR) (22.6%), resistant 1 2 classes (moderately [modR])...
The prevalence and impact of the overexpression AmpC efflux pumps were evaluated with a collection 190 Pseudomonas aeruginosa isolates recovered from bloodstream infections in 2008 multicenter study (10 hospitals) Spain. MICs panel 13 antipseudomonal agents determined by microdilution, expressions ampC, mexB, mexY, mexD, mexF real-time reverse transcription (RT)-PCR. Up to 39% overexpressed at least one mechanisms. ampC (24.2%) was most prevalent mechanism, followed mexY (13.2%), mexB...
Using a large multicenter prospective cohort of Pseudomonas aeruginosa bacteremia, we demonstrate that the exoU type III secretion system genotype is independently associated with increased risk early mortality bloodstream infections, whereas late multidrug-resistant profiles. Background. The (TTSS) major virulence determinant aeruginosa. objective this study was to determine whether TTSS useful prognostic marker P. bacteremia mortality. We also studied potential association between...
This study investigates the link between adaptation to biocides and antibiotics in Pseudomonas aeruginosa . An enrichment continuous culture of P. NCIMB 10421 (MIC 25 mg BKC l −1 ) was operated ( D =0.04 h , 792 h) with added benzalkonium chloride (BKC). A derivative, PA-29 (696 h), demonstrated a >12-fold decrease sensitivity biocide >350 ). The variant 256-fold increase resistance ciprofloxacin, mutation gyrA gene (Thr-83→Ile). Similarly, culturing original strain continuous-culture...
Background. Empirical combination therapy is recommended for patients with known or suspected Pseudomonas aeruginosa (PA) infection as a means to decrease the likelihood of administering inadequate antimicrobial treatment, prevent emergence resistance, and achieve possible additive even synergistic effect. Methods. We performed post hoc analysis PA bloodstream infections from published prospective cohort. Mortality was compared in treated adequate empirical definitive (AECT, ADCT),...
We report the genome of facultative intracellular parasite Rhodococcus equi, only animal pathogen within biotechnologically important actinobacterial genus Rhodococcus. The 5.0-Mb R. equi 103S is significantly smaller than those environmental rhodococci. This due to expansion in nonpathogenic species, via a linear gain paralogous genes and an accelerated genetic flux, rather reductive evolution equi. lacks extensive catabolic secondary metabolic complement rhodococci, it displays unique...
Emergence of epidemic clones and antibiotic resistance development compromises the management Pseudomonas aeruginosa cystic fibrosis (CF) chronic respiratory infections. Whole genome sequencing (WGS) was used to decipher phylogeny, interpatient dissemination, WGS mutator genotypes (mutome) resistome a widespread clone (CC274), in isolates from two highly-distant countries, Australia Spain, covering an 18-year period. The coexistence divergent CC274 clonal lineages revealed, but without...
ABSTRACT A limited number of Pseudomonas aeruginosa genotypes (mainly ST-111, ST-175, and ST-235), known as high-risk clones, are responsible for epidemics nosocomial infections by multidrug-resistant (MDR) or extensively drug-resistant (XDR) strains worldwide. We explored the potential biological parameters that may explain success these clones. total 20 isolates from each 4 resistance groups (XDR, MDR, ModR [resistant to 1 2 classes], MultiS [susceptible all antipseudomonals]), recovered a...
We investigated the presence of OprD mutations in 60 strains metallo-ß-lactamase-negative Pseudomonas aeruginosa intermediately susceptible (IS [n = 12]; MIC 8 μg/ml) or (S 48]; MICs ≤ 1 to 4 imipenem and/or meropenem that were isolated from patients with bacteremia order evaluate their impact on carbapenem susceptibility profiles. The oprD was detected by sequencing analysis. expression assessed both outer membrane protein (OMP) analysis and real-time PCR (RT-PCR). Fourteen (23%) isolates...
Whole-genome sequencing (WGS) was used for the characterization of frequently extensively drug resistant (XDR) Pseudomonas aeruginosa sequence type 175 (ST175) high-risk clone. A total 18 ST175 isolates recovered from 8 different Spanish hospitals were analyzed; 4 French included comparison. The typical resistance profile penicillins, cephalosporins, monobactams, carbapenems, aminoglycosides, and fluoroquinolones. In phylogenetic analysis, four clustered together with two one regions....
The pathogenic actinomycete Rhodococcus equi harbors different types of virulence plasmids associated with specific nonhuman hosts. We determined the complete DNA sequence a vapB(+) plasmid, typically pig isolates, and compared it that horse-specific vapA(+) plasmid type. pVAPB1593, circular 79,251-bp element, had same housekeeping backbone as but differed over an approximately 22-kb region. This variable region encompassed vap pathogenicity island (PAI), was clearly subject to selective...
Abstract Molecular typing of the actinomycete Rhodococcus equi is insufficiently developed, and little known about epidemiology transmission this multihost pathogen. We report a simple, reliable polymerase chain reaction system for R. based on 3 plasmid gene markers: traA from conserved conjugal transfer machinery vapA vapB, found in 2 different subpopulations. This “TRAVAP” scheme classifies into 4 categories: traA+/vapA+B−, traA+/vapA−B+, traA+/vapAB−, traA−/vapAB− (plasmidless). A TRAVAP...
ABSTRACT There is limited information on the role of penicillin-binding proteins (PBPs) in resistance Acinetobacter baumannii to β-lactams. This study presents an analysis allelic variations PBP genes A. isolates. Twenty-six clinical isolates (susceptible or resistant carbapenems) from three teaching hospitals Spain were included. The antimicrobial susceptibility profile, clonal pattern, and genomic species identification also evaluated. Based six complete genomes , identified, primers...
The aim of this study was to characterize carbapenem-resistant Klebsiella pneumoniae (CRKP) isolates recovered from clinical specimens at a tertiary care hospital in Egypt over period 15 months.Eight CRKP were included study. minimum inhibitory concentrations different antibiotics determined by broth microdilution and Etest methods. Multilocus sequence typing performed. Antibiotic resistance genes assessed PCR DNA sequencing. Plasmid analysis done S1 nuclease digestion whole genomic followed...
Antimicrobial resistance and the shortage of novel antibiotics have led to an urgent need for new antibacterial drug leads. Several existing natural product scaffolds (including chelocardins) not been developed because their suboptimal pharmacological properties could be addressed at time. It is demonstrated here that reviving such compounds through application biosynthetic engineering can deliver candidates. Through a rational approach, carboxamido moiety tetracyclines (an important...
ABSTRACT The activity of ceftazidime-avibactam was compared with that ceftazidime alone and meropenem against a collection 190 Pseudomonas aeruginosa clinical isolates recovered from multicenter study bloodstream infections. addition avibactam increased susceptibility in the complete strains (64.7% to 91.1%) particularly among subsets showing AmpC hyperproduction (10.9% 76.1%) or multidrug resistance (MDR) profiles (27% 77.8%). MICs ceftazidime-avibactam, contrast those meropenem, remained...
Antimicrobial resistance poses a significant challenge in modern medicine, affecting public health. Klebsiella pneumoniae infections compound this issue due to their broad range of and the emergence multiple antibiotic mechanisms. Efficient detection its capsular serotypes is crucial for immediate patient treatment, epidemiological tracking outbreak containment. Current methods have limitations that can delay interventions increase risk morbidity mortality. Raman spectroscopy promising...
Cyanobacterial blooms pose significant environmental and public health risks due to the production of toxins that contaminate water sources disrupt aquatic ecosystems. Rapid accurate identification cyanobacterial species is crucial for effective monitoring management strategies. In this study, we combined Raman spectroscopy with deep learning techniques classify four toxic species: Dolichospermum crassum, Aphanizomenon sp., Planktothrix agardhii Microcystis aeruginosa. Spectral data were...
Phytoplankton are essential to aquatic ecosystems but can cause harmful algal blooms (HABs) that threaten water quality, life, and human health. Developing new devices based on spectroscopic techniques offers a promising alternative for rapid accurate monitoring of environments. However, phytoplankton undergo various physiological changes throughout their life cycle, leading alterations in optical properties, such as autofluorescence. In this study, we present modification low-cost...
Background/Objectives: Antimicrobial resistance (AMR) poses a significant threat to global health and the economy, with projected costs ranging from $300 billion $1 trillion annually an estimated 10 million deaths per year by 2050. The food chain, primary production retail, represents critical entry point for antimicrobial resistant bacteria into communities. This underscores need coordinated “One Health” approach, integrating efforts in animal production, environmental health, human...