A5034888451- Cystic Fibrosis Research Advances
- Bacterial biofilms and quorum sensing
- Antibiotic Resistance in Bacteria
- Antifungal resistance and susceptibility
- Inhalation and Respiratory Drug Delivery
- Evolution and Genetic Dynamics
- Fungal Infections and Studies
- Pneumonia and Respiratory Infections
- Plant Pathogenic Bacteria Studies
- Sinusitis and nasal conditions
- Antimicrobial Resistance in Staphylococcus
- Tracheal and airway disorders
- Infections and bacterial resistance
- Bacterial Identification and Susceptibility Testing
- Bacterial Genetics and Biotechnology
- Bacteriophages and microbial interactions
- Genomics and Phylogenetic Studies
- Mycobacterium research and diagnosis
- Pediatric health and respiratory diseases
- Neutropenia and Cancer Infections
- Vibrio bacteria research studies
- Neonatal Respiratory Health Research
- Respiratory and Cough-Related Research
- Gut microbiota and health
- Asthma and respiratory diseases
Rigshospitalet
2016-2025
Novo Nordisk Foundation
2015-2025
Technical University of Denmark
2014-2025
Copenhagen University Hospital
2016-2025
University of Copenhagen
2014-2025
Clinical Microbiomics (Denmark)
2020
Regional Hospital Horsens
2019
Foundation Center
2015-2018
Cochrane
1998-2016
Cochrane
2006-2015
Summary Bacteria living as biofilm are frequently reported to exhibit inherent tolerance antimicrobial compounds, and might therefore contribute the persistence of infections. Antimicrobial peptides attracting increasing interest new potential therapeutics; however, little is known about mechanisms, which resistance or development towards these compounds in biofilms. Here we provide evidence that a spatially distinct subpopulation metabolically active cells Pseudomonas aeruginosa biofilms...
Laboratory evolution experiments have led to important findings relating organism adaptation and genomic evolution. However, continuous monitoring of long-term has been lacking for natural systems, limiting our understanding these processes in situ. Here we characterize the evolutionary dynamics a lineage clinically opportunistic bacterial pathogen, Pseudomonas aeruginosa , as it adapts airways several individual cystic fibrosis patients over 200,000 generations, provide estimates mutation...
Genome sequencing of bacterial pathogens has advanced our understanding their evolution, epidemiology, and response to antibiotic therapy. However, we still have only a limited knowledge the molecular changes in vivo evolving populations relation long-term, chronic infections. For example, it remains unclear what genes are mutated facilitate establishment long-term existence human host environment, which way acquisition hypermutator phenotype with enhanced rates spontaneous mutations...
spa typing of methicillin-resistant Staphylococcus aureus (MRSA) has traditionally been done by PCR amplification and Sanger sequencing the repeat region. At Hvidovre Hospital, Denmark, whole-genome (WGS) all MRSA isolates performed routinely since January 2013, an in-house analysis pipeline determines types. Due to national surveillance, are sent Statens Serum Institut, where type is determined sequencing. The purpose this study was evaluate reliability types obtained 150-bp paired-end...
Abstract Mutations in mexZ , encoding a negative regulator of the expression mexXY efflux pump genes, are frequently acquired by Pseudomonas aeruginosa at early stages lung infection. Although traditionally related to resistance first-line drug tobramycin, mutations associated with low-level aminoglycoside when determined laboratory, suggesting that their selection during infection may not be necessarily, or only, tobramycin therapy. Here, we show -mutated bacteria tend accumulate inside...
Background Ghost authorship, the failure to name, as an author, individual who has made substantial contributions article, may result in lack of accountability. The prevalence and nature ghost authorship industry-initiated randomised trials is not known. Methods Findings We conducted a cohort study comparing protocols corresponding publications for approved by Scientific-Ethical Committees Copenhagen Frederiksberg 1994–1995. defined present if individuals wrote trial protocol, performed...
The consequences of O-acetylated alginate-producing Pseudomonas aeruginosa biofilms in the lungs chronically infected cystic fibrosis (CF) patients are tolerance to both antibiotic treatments and effects on innate adaptive defense mechanisms. In clinical trials, azithromycin (AZM) has been shown improve lung function CF patients. present study was conducted accordance with previous vitro studies suggesting that effect AZM may be inhibition alginate production, blockage quorum sensing (QS),...
ABSTRACT A single mechanism of azole resistance was shown to predominate in clinical and environmental Aspergillus fumigatus isolates from the Netherlands, a link use azoles environment suggested. To explore prevalence azole-resistant A. other aspergilli European countries, we collected samples surroundings hospitals Copenhagen, Innsbruck, Madrid, flowerbeds an amusement park compost bags purchased Austria, Denmark, Spain screened for using multidish agars with itraconazole, voriconazole,...
A 6-year nationwide study of fungemia in Denmark was performed using data from an active surveillance program and laboratory information systems nonparticipating regions. total 2,820 episodes were recorded. The incidence increased 2004 to 2007 (7.7 9.6/100,000) decreased slightly 2008 2009 (8.7 8.6/100,000). highest incidences seen at the extremes age (i.e., 11.3 37.1/100,000 for those <1 70 79 years old, respectively). rate higher males than females (10.1 versus 7.6/100,000, P = 0.003),...
Pseudomonas aeruginosa can develop resistance to polymyxin and other cationic antimicrobial peptides. Previous work has shown that mutations in the PmrAB PhoPQ regulatory systems confer low moderate levels of (MICs 8 64 mg/liter) laboratory clinical strains this organism. To explore role high-level resistance, P. with colistin MICs > 512 mg/liter had been isolated from cystic fibrosis patients treated inhaled (polymyxin E) were analyzed. Probable loss-of-function phoQ alleles found these...
The growth dynamics of bacterial pathogens within infected hosts are a fundamental but poorly understood feature most infections. We have focused on the in situ distribution and characteristics two prevailing transmissible Pseudomonas aeruginosa clones that caused chronic lung infections cystic fibrosis (CF) patients for more than 20 years. used fluorescence hybridization (FISH) directly sputum specimens to examine spatial infecting P. cells. Mucoid variants were present as cell clusters...
ABSTRACT Pseudomonas aeruginosa can develop resistance to polymyxin and other cationic antimicrobial peptides. Previous work has shown that mutations in the PmrAB PhoPQ regulatory systems confer low moderate levels of colistin (polymyxin E) laboratory strains clinical isolates this organism (MICs 8 64 mg/liter). To explore role high-level resistance, P. from chronically colistin-treated cystic fibrosis patients, most with MICs >512 mg/liter, were analyzed. These contained probable...
The ability to establish lifelong persistent infections is a fundamental aspect of the interactions between many pathogenic microorganisms and their mammalian hosts. One example chronic lung by opportunistic pathogen Pseudomonas aeruginosa in cystic fibrosis (CF) patients. This infection process associated with extensive genetic adaptation microevolution infecting bacteria. Through investigations P. populations dynamics group CF patients followed at Danish Clinic Copenhagen, we have...
BACKGROUND AND METHODS: To assess the relation between seasonal variation and onset of initial chronic Pseudomonas aeruginosa infection, 300 Danish patients with cystic fibrosis were investigated. A retrospective analysis based on case reports was performed to identify date year P infection from 1965 1990. RESULTS: Sixty six per cent contracted their colonisation 68% during winter months (October March). Despite major changes in treatment, including improved intensified antibiotic...
This study investigated microbiological, clinical, and management issues outcomes for Danish fungemia patients. Isolates clinical information were collected at six centers. A total of 334 isolates, 316 episodes, 305 patients included, corresponding to 2/3 the national episodes. Blood culture positivity varied by system, species, procedure. Thus, cases with concomitant bacteremia reported less commonly BacT/Alert than Bactec system (9% [11/124 cases] versus 28% [53/192 cases]; P < 0.0001),...
ABSTRACT Pseudomonas aeruginosa causes a chronic infection in the lungs of cystic fibrosis (CF) patients by establishing an alginate-containing biofilm. The has been studied several animal models; however, most models required artificial embedding bacteria. We present here new pulmonary mouse model without embedding. is based on stable mucoid CF sputum isolate (NH57388A) with hyperproduction alginate due to deletion mucA and functional N -acylhomoserine lactone (AHL)-based quorum-sensing...