A5054646768- Bacterial Genetics and Biotechnology
- Microbial Metabolic Engineering and Bioproduction
- Bacteriophages and microbial interactions
- Antibiotic Resistance in Bacteria
- DNA Repair Mechanisms
- Advanced biosensing and bioanalysis techniques
- Enzyme Catalysis and Immobilization
- RNA and protein synthesis mechanisms
- DNA and Nucleic Acid Chemistry
- Lipid metabolism and biosynthesis
- Genomics and Phylogenetic Studies
- Biofuel production and bioconversion
- Enzyme Structure and Function
- Microbial Natural Products and Biosynthesis
- Photosynthetic Processes and Mechanisms
- CRISPR and Genetic Engineering
- Genomics and Chromatin Dynamics
- Enzyme Production and Characterization
- RNA Interference and Gene Delivery
- Vibrio bacteria research studies
- Machine Learning in Bioinformatics
- Escherichia coli research studies
- Metabolomics and Mass Spectrometry Studies
- Protein Kinase Regulation and GTPase Signaling
- Caveolin-1 and cellular processes
Instituto de Biomedicina y Biotecnología de Cantabria
2012-2023
Universidad de Cantabria
2012-2023
Consejo Superior de Investigaciones Científicas
2010-2019
Sodercan
2013
Centro Nacional de Biotecnología
2011
Scripps Research Institute
2002-2011
Instituto de Salud Carlos III
2008
Spanish National Cancer Research Centre
2005-2006
Centro de Investigaciones Biológicas Margarita Salas
1999-2006
We investigated the presence of OprD mutations in 60 strains metallo-ß-lactamase-negative Pseudomonas aeruginosa intermediately susceptible (IS [n = 12]; MIC 8 μg/ml) or (S 48]; MICs ≤ 1 to 4 imipenem and/or meropenem that were isolated from patients with bacteremia order evaluate their impact on carbapenem susceptibility profiles. The oprD was detected by sequencing analysis. expression assessed both outer membrane protein (OMP) analysis and real-time PCR (RT-PCR). Fourteen (23%) isolates...
The F plasmid is the foremost representative of a large group conjugative plasmids, prevalent in Escherichia coli, and widely distributed among Enterobacteriaceae. These plasmids are clinical relevance, given their frequent association with virulence determinants, colicins, antibiotic resistance genes. Originally defined by sensitivity to certain male-specific phages, IncF share conserved system regulatory circuits. In order determine whether genetic architecture regulation circuits...
TrwB is the conjugative coupling protein of plasmid R388. TrwBΔN70 contains soluble domain TrwB. It was constructed by deletion <i>trwB</i> sequences containing N-proximal transmembrane segments. Purified bound tightly fluorescent ATP analogue TNP-ATP (<i>K</i> <sub>s</sub> = 8.7 μm) but did not show measurable ATPase or GTPase activity. A single binding site found per monomer. An intact ATP-binding essential for R388 conjugation, since a mutant with amino acid alteration in signature...
The conjugative plasmid R388 and a number of other plasmids carry an operon, stbABC, adjacent to the origin transfer. We investigated role stbA, stbB, stbC genes. Deletion stbA affected both conjugation stability. It led 50-fold increase in transfer frequency, as well high loss. In contrast, deletion stbB abolished but provoked no change showed effect, neither nor entire stb operon had effect on conjugation, which remained wild-type plasmid, loss phenotype similar that R388ΔstbA mutant....
BACKGROUND Spent sulfite liquor is a by-product obtained in the process of manufacturing dissolving pulp by acid method. A full physico-chemical characterization this waste has been developed order to determine if it susceptible valorization means biorefinery processes. RESULTS The main components present spent are sugars from hemicelluloses (25.9%), mainly xylose (18.2%) because hardwood used as raw material, and lignosulfonates (50.6%). Taking into account results obtained, literature...
Summary Horizontal transfer of antibiotic resistance genes carried by conjugative plasmids poses a serious health problem. As relaxases are transported to recipient cells during bacterial conjugation, we investigated whether blocking relaxase activity in the cell might inhibit conjugation. For that purpose, used an intrabody approach generating single‐chain Fv antibody library against TrwC plasmid R388. Recombinant antibodies were engineered for cytoplasmic expression Escherichia coli and...
DNA-binding proteins are promising reagents for the sequence-specific modification of DNA-based nanostructures. Here, we investigate utility a series relaxase proteins-TrwC, TraI, and MobA-for nanofunctionalization. Relaxases involved in conjugative transfer plasmids between bacteria, bind to their DNA target sites via covalent phosphotyrosine linkage. We study binding relaxases two standard origami structures-rodlike six-helix bundles flat rectangular sheets. find highly orthogonal with...
Bacterial conjugation is an example of macromolecular trafficking between cells and responsible for the spreading antibiotic resistance among bacteria. It involves translocation single-stranded DNA across membranes through a type IV secretion system. A coupling protein links DNA-processing nucleoprotein complex, relaxosome, with transport apparatus during cell mating. In <i>Escherichia coli</i> plasmid R388 such TrwB, basic integral inner-membrane nucleoside-triphosphate-binding protein....
Plasmids, when transferred by conjugation in natural environments, must overpass restriction-modification systems of the recipient cell. We demonstrate that protein ArdC, encoded broad host range plasmid R388, was required for from Escherichia coli to Pseudomonas putida. Expression ardC cells, but not donor cells. Besides, if hsdRMS system deleted P. putida also present E. Thus, ArdC has antirestriction activity against HsdRMS and consequently broadens R388 range. The crystal structure...