A5078278469- Advanced biosensing and bioanalysis techniques
- RNA Interference and Gene Delivery
- DNA and Nucleic Acid Chemistry
- Molecular Junctions and Nanostructures
- Bacteriophages and microbial interactions
- RNA and protein synthesis mechanisms
- Nanopore and Nanochannel Transport Studies
- Gene Regulatory Network Analysis
- CRISPR and Genetic Engineering
- Plasmonic and Surface Plasmon Research
- Lipid Membrane Structure and Behavior
- Microfluidic and Bio-sensing Technologies
- Modular Robots and Swarm Intelligence
- Quantum and electron transport phenomena
- Biosensors and Analytical Detection
- Molecular Communication and Nanonetworks
- Bacterial Genetics and Biotechnology
- DNA and Biological Computing
- Electrostatics and Colloid Interactions
- 3D Printing in Biomedical Research
- Microfluidic and Capillary Electrophoresis Applications
- Innovative Microfluidic and Catalytic Techniques Innovation
- Semiconductor Quantum Structures and Devices
- Supramolecular Self-Assembly in Materials
- Quantum-Dot Cellular Automata
Technical University of Munich
2016-2025
Max Planck School Matter to Life
2024
Nanosystems Initiative Munich
2014-2019
Seattle Pacific University
2019
University of Nebraska at Omaha
2019
Max Planck Computing and Data Facility
2016
Ludwig-Maximilians-Universität München
2000-2013
Center for NanoScience
2000-2013
Schott (Germany)
2010-2012
National Centre for Biological Sciences
2011
DNA origami is a powerful method for the programmable assembly of nanoscale molecular structures. For applications these structures as functional biomaterials, study reaction kinetics and dynamic processes in real time with high spatial resolution becomes increasingly important. We present single-molecule assay binding unbinding on origami. find that hybridization to single-stranded extensions similar isolated substrate-immobilized slight position dependence On basis knowledge kinetics, we...
We created nanometer-scale transmembrane channels in lipid bilayers by means of self-assembled DNA-based nanostructures. Scaffolded DNA origami was used to create a stem that penetrated and spanned membrane, as well barrel-shaped cap adhered the part via 26 cholesterol moieties. In single-channel electrophysiological measurements, we found similarities response natural ion channels, such conductances on order 1 nanosiemens channel gating. More pronounced gating seen for mutations which...
We describe kinetic control of DNA hybridization: loop complexes are used to inhibit the hybridization complementary oligonucleotides; rationally designed catalysts shown be effective in promoting their hybridization. This is basis a strategy for using as fuel drive free-running artificial molecular machines.
The use of dynamic, self-assembled DNA nanostructures in the context nanorobotics requires fast and reliable actuation mechanisms. We therefore created a 55-nanometer-by-55-nanometer DNA-based molecular platform with an integrated robotic arm length 25 nanometers, which can be extended to more than 400 nanometers actuated externally applied electrical fields. Precise, computer-controlled switching between arbitrary positions on achieved within milliseconds, as demonstrated single-pair...
We study the distance-dependent quenching of fluorescence due to a metallic nanoparticle in proximity fluorophore. In our single-molecule measurements, we achieve excellent control over structure and stoichiometry by using self-assembled DNA structures (DNA origami) as breadboard where both fluorophore 10 nm are positioned with nanometer precision. The spectroscopy method employed here reports on co-localization particle dye, while lifetime imaging is used directly obtain correlation...
Resolving the distances: Rectangular DNA origami labeled with fluorophores at specific positions has been used as a nanoscopic ruler. Super-resolution microscopy based on subsequent localization of single molecules enables two distance about 90 nm to be optically resolved. This combination subdiffraction imaging and nanotechnology opens up new avenues for studying nanostructures their dynamics. Detailed facts importance specialist readers are published ”Supporting Information”. Such...
The realization of artificial biochemical reaction networks with unique functionality is one the main challenges for development synthetic biology. Due to reduced number components, circuits constructed in vitro promise be more amenable systematic design and quantitative assessment than embedded within living organisms. To make good on that promise, effective methods composing subsystems into larger systems are needed. Here we used an oscillator based transcription RNA degradation reactions...
DNA-based nanopores are synthetic biomolecular membrane pores, whose geometry and chemical functionality can be tuned using the tools of DNA nanotechnology, making them promising molecular devices for applications in single-molecule biosensing biology. Here we introduce a large channel with an ≈4 nm diameter pore, which has stable electrical properties spontaneously inserts into flat lipid bilayer membranes. Membrane incorporation is facilitated by number hydrophobic functionalizations or,...
Abstract To impart directionality to the motions of a molecular mechanism, one must overcome random thermal forces that are ubiquitous on such small scales and in liquid solution at ambient temperature. In equilibrium without energy supply, directional motion cannot be sustained violating laws thermodynamics. Under conditions away from thermodynamic equilibrium, may achieved within framework Brownian ratchets, which diffusive mechanisms have broken inversion symmetry 1–5 . Ratcheting is...
Abstract Transmembrane nanostructures like ion channels and transporters perform key biological functions by controlling flow of molecules across lipid bilayers. Much work has gone into engineering artificial nanopores applications in selective gating molecules, label-free detection/sensing biomolecules DNA sequencing have shown promise. Here, we use origami to create a synthetic 9 nm wide nanopore, controlled programmable, lipidated flaps equipped with size-selective system for the...
A DNA aptamer structure is the basis of a molecular machine that can be instructed to grab or release human blood-clotting factor α-thrombin, depending on operator sequence addressing it. In picture structure, which assumes conformation characterized by two stacked guanine quadruplex structures, linked thrombin (gray ellipse).
We present measurements on the Kondo effect in a small quantum dot connected strongly to one lead and weakly other. The conductance of reveals an offset resonance at zero magnetic field. While persists negative bias regime, it is suppressed opposite direction. This demonstrates pinning Fermi levels leads.
Abstract The molecular recognition properties of DNA molecules combined with the distinct mechanical single and double strands can be utilized for construction nanodevices, which perform ever more tasks possible applications ranging from nanoconstruction to intelligent drug delivery. With help it is construct machinelike devices that are capable rotational motion, pulling stretching, or even unidirectional motion. It devise autonomous grab release molecules, also simple information‐processing tasks.
pH oscillations generated by a nonequilibrium chemical reaction are used to switch pH-sensitive DNA structure between two distinct conformations. The utilization of oscillator represents novel method for achieving autonomous motion in molecular devices. oscillatory is variant the Landolt and produces variations range 5 7. In this range, cytosine-rich strand can be switched random coil conformation folded i-motif structure. conformational changes monitored simultaneously with value...
Abstract The arrangement of DNA‐based nanostructures into extended higher order assemblies is an important step towards their utilization as functional molecular materials. We herein demonstrate that by electrostatically controlling the adhesion and mobility DNA origami structures on mica surfaces simple addition monovalent cations, large ordered 2D arrays tiles can be generated. lattices formed either close‐packing symmetric, non‐interacting structures, or utilizing blunt‐end stacking...