A5052548866- Advanced Fluorescence Microscopy Techniques
- Advanced biosensing and bioanalysis techniques
- Advanced Electron Microscopy Techniques and Applications
- RNA Interference and Gene Delivery
- Near-Field Optical Microscopy
- Advanced Biosensing Techniques and Applications
- Monoclonal and Polyclonal Antibodies Research
- Plasmonic and Surface Plasmon Research
- DNA and Nucleic Acid Chemistry
- Bacteriophages and microbial interactions
- Force Microscopy Techniques and Applications
- Cell Image Analysis Techniques
- RNA and protein synthesis mechanisms
- Molecular Junctions and Nanostructures
- Cell Adhesion Molecules Research
- Single-cell and spatial transcriptomics
- Genomics and Chromatin Dynamics
- T-cell and B-cell Immunology
- Nuclear Structure and Function
- Glycosylation and Glycoproteins Research
- Viral gastroenteritis research and epidemiology
- Cardiomyopathy and Myosin Studies
- Bacterial Genetics and Biotechnology
- Microtubule and mitosis dynamics
- Molecular Biology Techniques and Applications
Max Planck Institute of Biochemistry
2016-2025
Ludwig-Maximilians-Universität München
2016-2025
Center for NanoScience
2016-2025
Aarhus University
2023
Max Planck Society
2017-2022
LMU Klinikum
2020
St Martins Hospital
2020
Technical University of Munich
2008-2019
Harvard University
2012-2017
Center for Systems Biology
2012-2017
DNA origami is a powerful method for the programmable assembly of nanoscale molecular structures. For applications these structures as functional biomaterials, study reaction kinetics and dynamic processes in real time with high spatial resolution becomes increasingly important. We present single-molecule assay binding unbinding on origami. find that hybridization to single-stranded extensions similar isolated substrate-immobilized slight position dependence On basis knowledge kinetics, we...
Single-molecule localization microscopy (SMLM) describes a family of powerful imaging techniques that dramatically improve spatial resolution over standard, diffraction-limited and can image biological structures at the molecular scale. In SMLM, individual fluorescent molecules are computationally localized from sequences localizations used to generate super-resolution or time course images, define trajectories. this Primer, we introduce basic principles SMLM before describing main...
Phasing-in quality control in the nucleus The fundamental process of protein is not well understood. contains several non–membrane-bound subcompartments forming liquid-like condensates. largest these nucleolus, site ribosome biogenesis. Frottin et al. found that metastable nuclear proteins misfold upon heat stress enter nucleolus. In they avoid irreversible aggregation and remain competent for shock 70–dependent refolding recovery from stress. Prolonged or uptake associated with...
Abstract Specialized epitope tags are widely used for detecting, manipulating or purifying proteins, but often their versatility is limited. Here, we introduce the ALFA-tag, a rationally designed tag that serves remarkably broad spectrum of applications in life sciences while outperforming established like HA-, FLAG®- myc-tag. The ALFA-tag forms small and stable α-helix functional irrespective its position on target protein prokaryotic eukaryotic hosts. We characterize nanobody (NbALFA)...
Push Me, Release, Pull You In eukaryotic cells, nearly all long-distance transport of cargos is carried out by the microtubule-based motors kinesin and dynein. These opposite-polarity move bidirectionally so that they reach their cellular destinations with spatial temporal specificity. To understand motor ensembles, Derr et al. (p. 662 , published online 11 October; see Persective Diehl ) used a DNA scaffold for building an artificial cargo could be programmed to bind different numbers types...
Abstract Fluorescence in situ hybridization (FISH) is a powerful single-cell technique for studying nuclear structure and organization. Here we report two advances FISH-based imaging. We first describe the visualization of single-copy regions genome using single-molecule super-resolution methodologies. then introduce robust reliable system that harnesses single-nucleotide polymorphisms (SNPs) to visually distinguish maternal paternal homologous chromosomes mammalian insect systems. Both...
DNA self-assembly has produced diverse synthetic three-dimensional polyhedra. These structures typically have a molecular weight no greater than 5 megadaltons. We report simple, general strategy for one-step of wireframe polyhedra that are more massive most previous structures. A stiff three-arm-junction origami tile motif with precisely controlled angles and arm lengths was used hierarchical assembly experimentally constructed tetrahedron (20 megadaltons), triangular prism (30 cube (40...
Resolving the distances: Rectangular DNA origami labeled with fluorophores at specific positions has been used as a nanoscopic ruler. Super-resolution microscopy based on subsequent localization of single molecules enables two distance about 90 nm to be optically resolved. This combination subdiffraction imaging and nanotechnology opens up new avenues for studying nanostructures their dynamics. Detailed facts importance specialist readers are published ”Supporting Information”. Such...
The realization of artificial biochemical reaction networks with unique functionality is one the main challenges for development synthetic biology. Due to reduced number components, circuits constructed in vitro promise be more amenable systematic design and quantitative assessment than embedded within living organisms. To make good on that promise, effective methods composing subsystems into larger systems are needed. Here we used an oscillator based transcription RNA degradation reactions...
We report the development of multiplexed cellular super-resolution imaging using DNA-barcoded binders.
Fluorescence microscopy, with its molecular specificity, is one of the major characterization methods used in life sciences to understand complex biological systems. Super-resolution approaches
To understand biological processes, it is necessary to reveal the molecular heterogeneity of cells by gaining access location and interaction all biomolecules. Significant advances were achieved super-resolution microscopy, but such methods are still far from reaching multiplexing capacity proteomics. Here, we introduce secondary label-based unlimited multiplexed DNA-PAINT (SUM-PAINT), a high-throughput imaging method that capable achieving virtually at better than 15 nm resolution. Using...