A5007941136- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Genomics and Chromatin Dynamics
- RNA modifications and cancer
- CRISPR and Genetic Engineering
- Cancer-related molecular mechanisms research
- RNA Research and Splicing
- Epigenetics and DNA Methylation
- Chromosomal and Genetic Variations
- Genetics and Neurodevelopmental Disorders
- Animal Genetics and Reproduction
- RNA and protein synthesis mechanisms
- Genetic Syndromes and Imprinting
- Pluripotent Stem Cells Research
- interferon and immune responses
- Renal and related cancers
- Prenatal Screening and Diagnostics
- Congenital heart defects research
- Genomic variations and chromosomal abnormalities
- RNA Interference and Gene Delivery
- Gender Studies in Language
- MicroRNA in disease regulation
- RNA regulation and disease
- Nuclear Structure and Function
- Mitochondrial Function and Pathology
- Chromatin Remodeling and Cancer
Harvard University
2016-2025
Massachusetts General Hospital
2016-2025
Boston VA Research Institute
2019-2025
Boston University
1997-2024
Cedars-Sinai Medical Center
2024
Harvard University Press
1999-2023
Howard Hughes Medical Institute
2010-2019
Kanazawa University
2008
University of Washington
1999-2000
Case Western Reserve University
2000
To equalize X-chromosome dosages between the sexes, female mammal inactivates one of her two X chromosomes. inactivation (XCI) is initiated by expression Xist, a 17-kb noncoding RNA (ncRNA) that accumulates on in cis. Because interacting factors have not been isolated, mechanism which Xist induces silencing remains unknown. We discovered 1.6-kilobase ncRNA (RepA) within and identified Polycomb complex, PRC2, as its direct target. PRC2 initially recruited to RepA RNA, with Ezh2 serving...
Protein partners for chromosome silencing Female mammals have two X chromosomes, one of which is almost completely shut down during development. The long noncoding Xist RNA plays a role in this process. To understand how whole can be stably inactivated, Minajigi et al. identified many the proteins that bind to RNA, include cohesins. Paradoxically, interaction between and cohesin subunits resulted repulsion complexes from inactive chromosome, changing three-dimensional shape chromosome....
Mammalian X inactivation turns off one female chromosome to enact dosage compensation between XX and XY individuals. is known be regulated in cis by Xite, Tsix, Xist, but principle the two Xs must also trans ensure mutually exclusive silencing. Here, we demonstrate that interchromosomal pairing mediates this communication. Pairing occurs transiently at onset of specific X-inactivation center. Deleting Xite Tsix perturbs counting/choice, whereas their autosomal insertion induces de novo...
Abstract Fluorescence in situ hybridization (FISH) is a powerful single-cell technique for studying nuclear structure and organization. Here we report two advances FISH-based imaging. We first describe the visualization of single-copy regions genome using single-molecule super-resolution methodologies. then introduce robust reliable system that harnesses single-nucleotide polymorphisms (SNPs) to visually distinguish maternal paternal homologous chromosomes mammalian insect systems. Both...
Transcriptome studies are revealing that the eukaryotic genome actively transcribes a diverse repertoire of large noncoding RNAs (ncRNAs), many which unannotated and distinct from small have garnered much attention in recent years. Why they so pervasive, do function? X-chromosome inactivation (XCI) is classic epigenetic phenomenon associated with ncRNAs. Here, I provide perspective on how XCI achieved mice suggest this knowledge can be applied to rest genome. Emerging data indicate long...