A5049552049- RNA modifications and cancer
- CRISPR and Genetic Engineering
- RNA Research and Splicing
- Cancer-related molecular mechanisms research
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- MicroRNA in disease regulation
- Immune Cell Function and Interaction
- Genomics and Chromatin Dynamics
- Epigenetics and DNA Methylation
- Single-cell and spatial transcriptomics
- Chromosomal and Genetic Variations
- Pluripotent Stem Cells Research
- Virus-based gene therapy research
- Immunotherapy and Immune Responses
- Retinal Development and Disorders
- Molecular Biology Techniques and Applications
- Cancer Immunotherapy and Biomarkers
- Cancer Research and Treatments
- Cancer Mechanisms and Therapy
- RNA Interference and Gene Delivery
- Protease and Inhibitor Mechanisms
- Coronary Artery Anomalies
- IL-33, ST2, and ILC Pathways
- Congenital heart defects research
- Advanced biosensing and bioanalysis techniques
The Fourth Affiliated Hospital of Soochow University (Suzhou Dushu Lake Hospital)
2024
Soochow University
2014-2024
Second Affiliated Hospital of Soochow University
2024
Children's Hospital of Fudan University
2007-2023
Yangtze University
2022
Shandong University of Traditional Chinese Medicine
2022
Affiliated Hospital of Shandong University of Traditional Chinese Medicine
2022
Nanyang Technological University
2011-2021
Center for Excellence in Molecular Plant Sciences
2021
Chinese Academy of Sciences
2021
Joint profiling of transcriptome and chromatin accessibility within single cells allows for the deconstruction complex relationship between transcriptional states upstream regulatory programs determining different cell fates. Here, we developed an automated method with high sensitivity, assay single-cell regions (ASTAR-seq), simultaneous measurement whole-cell same cell. To show utility ASTAR-seq, profiled 384 mESCs under naive primed pluripotent as well a two-cell like state, 424 human...
H3.3 is a histone variant, which deposited on genebodies and regulatory elements, by Hira, marking active transcription. Moreover, heterochromatin Atrx/Daxx complex. The exact role of in cell fate transition remains elusive. Here, we investigate the dynamic changes deposition variant during cellular reprogramming. maintains identities parental cells reprogramming as its removal at early time-point enhances efficiency process. We find that plays similar transdifferentiation to hematopoietic...
Abstract N6-methyladenosine (m 6 A), the most abundant internal modification in eukaryotic mRNA, is installed by a multi-component writer complex; however, exact roles of each component remain poorly understood. Here we show that potential E3 ubiquitin ligase Hakai colocalizes and interacts with other m A components, mutants exhibit typical pathway defects Drosophila , such as lowered levels aberrant Sxl alternative splicing, wing behavior defects. Hakai, Vir, Fl(2)d Flacc form stable...
Integrative single-cell analysis de-constructs the heterogeneity of human reprogramming and uncovers its stage-specific GRNs.
<sec> <title>BACKGROUND</title> Diabetes Mellitus is a major non-communicable disease with significant increase in prevalence, especially low and middle-income countries. The latest IDF Atlas (2025) reports that 11,1%, or about 1 9, of the adult population (20-79 years old) living diabetes, over 4 10 unaware they have condition. This Prevalence expected to 8 adults by year 2050. Early diagnosis treatment reduces risk slows progression debilitating complications such as amputation, vision...
Dopamine D₂ receptor (DRD2) is important for normal function of the brain reward circuit. Lower DRD2 in increases risk substance abuse, obesity, attention deficit/hyperactivity disorder, and depression. Moreover, target most antipsychotics currently use. It well known that dopamine-induced endocytosis its desensitization. However, it remains controversial whether recycled back to plasma membrane or targeted degradation following dopamine stimulation. Here, we used total internal reflection...
Tumor-associated macrophages (TAMs) are known to regulate tumor response many anti-cancer therapies, including oncolytic virotherapy. Oncolytic virotherapy employing paramyxoviruses, such as attenuated measles (MeV) and mumps (MuV) viruses, has demonstrated therapeutic potential against various malignancies. However, the of TAMs paramyxoviruses consequent effect on virotherapeutic efficacy remains be characterized. Here, we demonstrate that presence human monocyte-derived (MDMs),...
Telomeric regions are known to be transcribed in several organisms. Although originally reported from all chromosomes with enrichment near the inactive X of female cells, we show that telomeric RNAs fact enriched on both sex mouse a developmentally specific manner. In stem active Xs marked by RNAs. male and Y accumulate RNA. Distribution changes during cell differentiation, after which they associate only heterochromatic each sex. FISH mapping suggests accumulated localize at distal end....
Oncolytic measles and mumps viruses (MeV, MuV) have a potential for anti-cancer treatment. We examined the anti-tumor activity of MeV, MuV, MeV-MuV combination (MM) against human solid malignancies (HSM). MM targeted significantly killed various cancer cell lines HSM but not normal cells. demonstrated greater effect prolonged survival in prostate xenograft tumor model compared to MeV MuV. induced expression immunogenic death markers enhanced spleen-infiltrating immune In conclusion, improves...
The correlation between superoxide dismutase 2 (SOD2) V16A variant and urological cancer susceptibility has been widely studied, however, with divergent results.Totally, 9,910 patients 11,239 control subjects were enrolled. is associated an increased to (A-allele vs. V-allele: OR = 1.06, 95% CI 1.00 - 1.13, P 0.047; AA+AV VV: 1.09, 1.02 1.16, 0.008), especially for prostate (PCa). Serum SOD2 level of PCa VV+VA genotypes was lower than in those AA genotypes. expression downregulated both...
Purpose. A recently identified colistin resistance gene, mcr-1, has been reported in many countries. In this study, we established a new real-time PCR method to detect it. Methodology. We used the mcr-1 gene variety of isolates and faecal samples from 20 provinces municipal cities China. Results. Of 2330 (from 10 species) screened, 54 (2.3 %) were positive for mcr-1. All mcr-1-positive that belonged Escherichia coli strains, among which 9, 1, 44 as enteropathogenic E. coli, enteroadherent...
We double-tagged Xist (inactivated X chromosome-specific transcript), a prototype long non-coding RNA pivotal for chromosome inactivation (XCI), using the programmable sequence binding domain of Pumilio protein, one tag live-cell imaging and other replacing A-repeat (a critical Xist) to generate "ΔA mutant" tether effector proteins dissecting functionality. Based on observation in live cells that induced XCI undifferentiated embryonic stem (ES) is counteracted by intrinsic reactivation...
// Ye Li 1, 2, * , Xu Zhang 3, 4, Jian Yang Yi 1 Dongming Zhu 2 Lifeng Yanbo 5 Dechun and Zhou Department of General Surgery, The First Affiliated Hospital Soochow University, Suzhou 215006, China Pancreatic Disease Research Centre, 3 Science & Technology Town Hospital, 4 to Nanjing Medical Oncology, These authors have contributed equally this work Correspondence to: Zhou, email: zhoujian20150602@126.com Keywords: BNIP3, HIF-1α, apoptosis, methylation, pancreatic cancer Received:...
Epigenetic changes have been recently recognized as important in many human cancers. Enhancer of zeste homologue 2 (EZH2)gene has shown overexpression various cancers, consistent with a straightforward role EZH2 an oncogene, but its function carcinogenesis is partly contradictory. The development colorectal cancer (CRC) not yet clarified. In present study, we observed up-regulation expression tumor tissues from CRC patients [corrected]. cell lines the trend using RT-PCR. We showed that TNM...
With the advances in high-throughput DNA sequencing technologies, RNA-seq has rapidly emerged as a powerful tool for quantitative analysis of gene expression and transcript variant discovery. In comparative experiments, differential is commonly performed on data to identify genes/features that are differentially expressed between biological conditions. Most existing statistical methods parametric assume either Poisson distribution or negative binomial read counts. However, violation...
Hydrogen is a therapeutic antioxidant that has been used extensively in clinical trials. It also acts as bioactive molecule can alleviate abiotic stress plants. However, the biological effects of hydrogen somatic embryos and underlying molecular basis remain largely unknown. In this study, morphological physiological influence exogenous H₂ treatment during embryogenesis was characterized Larix leptolepis Gordon. The results showed exposure to increased proportions active pro-embryogenic...
Patterning of the animal embryo's antero-posterior (AP) axis is dependent on spatially and temporally regulated Hox gene expression. The murine Hoxd4 has been proposed to harbour two promoters, an upstream promoter P2, a downstream P1, that lie 5.2 1.1 kilobase pairs (kb) coding region respectively. evolutionarily conserved microRNA-10b (miR-10b) lies in genomic locus intron separating non-coding exons 4 5 P2 transcript directly adjacent P1 promoter. transcription by 3′ neural enhancer...