A5006160118- Chromosomal and Genetic Variations
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Genetic and phenotypic traits in livestock
- Genomics and Phylogenetic Studies
- Genetic Mapping and Diversity in Plants and Animals
- Genetic diversity and population structure
- Epigenetics and DNA Methylation
- Animal Genetics and Reproduction
- Genetic Associations and Epidemiology
- Genomics and Chromatin Dynamics
- Genetic Syndromes and Imprinting
- Biochemical and Molecular Research
- Genetics and Neurodevelopmental Disorders
- Amphibian and Reptile Biology
- Wildlife Ecology and Conservation
- Polyamine Metabolism and Applications
- Fish Ecology and Management Studies
- Reproductive Biology and Fertility
- Hormonal Regulation and Hypertension
- Glutathione Transferases and Polymorphisms
- Cancer-related molecular mechanisms research
- Environmental DNA in Biodiversity Studies
- Trypanosoma species research and implications
- Physiological and biochemical adaptations
- RNA Research and Splicing
Texas A&M University
2014-2025
Mitchell Institute
2006-2011
Materials Systems (United States)
2011
University of Iowa
2011
Integrated DNA Technologies (United States)
2007-2011
Texas Biomedical Research Institute
1996-2009
The University of Texas at San Antonio
2007
The University of Texas Health Science Center at San Antonio
1996-2004
Boston University
2004
University of Pittsburgh
2004
The first sequenced marsupial genome promises to reveal unparalleled insights into mammalian evolution. We have used the Monodelphis domestica (gray short-tailed opossum) sequence construct map of a major histocompatibility complex (MHC). MHC is most gene-dense region and critical immunity reproductive success. bridges phylogenetic gap between eutherian mammals minimal essential birds. Here we show that opossum gene dense complex, as in humans, but shares more organizational features with...
Over the last 20-80 million years mammalian placenta has taken on a variety of morphologies through both divergent and convergent evolution. Recently we have shown that human genome unique epigenetic pattern large partially methylated domains (PMDs) highly (HMDs) with gene body DNA methylation positively correlating level expression. In order to determine evolutionary conservation patterns transcriptional regulatory programs in placenta, performed genome-wide methylome (MethylC-seq) analysis...
Abstract XX female and XY male therian mammals equalize X-linked gene expression through the mitotically-stable transcriptional inactivation of one two X chromosomes in somatic cells. Here, we describe an essential function homolog ancestral X-Y pair, Kdm5c - Kdm5d , Xist lncRNA, which is required for stable X-inactivation. Ablation females results a significant reduction RNA expression. encodes demethylase that enhances by converting histone H3K4me2/3 modifications into H3K4me1. Ectopic...
Abstract Increased adiposity has repeatedly been identified as a major risk factor for variety of chronic diseases. However, the question still remains whether amount adipose tissue itself is genetically mediated. To address this question, segregation analysis, using maximum likelihood techniques implemented in computer program Pedigree Analysis Package (PAP), was performed on fat mass (kilograms body fat) large sample extended Mexican American families residing San Antonio, TX. The only...
Abstract We performed a genome scan using BMD data of the forearm and hip on 664 individuals in 29 Mexican-American families. obtained evidence for QTL chromosome 4p, affecting overall, chromosomes 2p 13q, men. Introduction: The San Antonio Family Osteoporosis Study (SAFOS) was designed to identify genes environmental factors that influence bone mineral density (BMD) from large Materials Methods: genome-wide linkage analysis 416 highly polymorphic microsatellite markers spaced approximately...
Abstract The genetic mechanisms that control variation in blood pressure level are largely unknown. One of the first steps understanding those is localization genes have a significant effect on pressure. We performed genome scans systolic (SBP) and diastolic (DBP) population‐based sample families San Antonio Family Heart Study. A likelihood‐based Mendelian model incorporating genotype‐specific effects sex, age, age 2 , BMI, (SBP or DBP, as appropriate) covariates was used to perform...
Evidence from a few genes in diverse species suggests that X-chromosome inactivation (XCI) marsupials is characterized by exclusive, but leaky of the paternally derived X chromosome. To study phenomenon marsupial XCI more comprehensively, we profiled parent-of-origin allele-specific expression, DNA methylation, and histone modifications fetal brain extra-embryonic membranes gray, short-tailed opossum ( Monodelphis domestica ). The majority X-linked (152 176 with trackable SNP variants)...
Abstract Genomic imprinting is a parent-of-origin-specific expression phenomenon that plays fundamental roles in many biological processes. In animals, only observed therian mammals, with ∼200 imprinted genes known humans and mice. The pattern marsupials has been minimally investigated by examining orthologs to eutherian genes. To identify marsupial-specific an unbiased way, we performed RNA-seq studies on samples of fetal brain placenta from the reciprocal cross progeny two laboratory...
The laboratory opossum, Monodelphis domestica , serves as a critical marsupial model in biomedical research. Proper feeding approaches are essential for promoting animal growth and wellbeing. In this study, we systematically evaluated food scattering potential contamination from feces across 4 methods: direct placement of pellets on bedding using 3 different types containers. We conducted timed daily observations marking behaviors 22 animals, capturing images by photograph at specific...
Thyroid hormones play major roles in the regulation of a wide range metabolic and physiologic processes, but genes environmental factors that affect normal, quantitative variation thyroid hormone concentrations are largely unknown. Using genetic methods, we evaluated effects on 586 women 425 men from 27 randomly ascertained Mexican-American families San Antonio Family Heart Study. Data were available free total T4, T3, TSH, thyroglobulin, T4-binding globulin, as well covariates, including...
Abstract In eutherian mammals, the X and Y chromosomes undergo meiotic sex chromosome inactivation (MSCI) during spermatogenesis in males. However, following fertilization, both paternally (Xp) maternally (Xm) inherited are active inner cell mass of female blastocyst, then random one occurs each cell, leading to a mosaic pattern X‐chromosome activity adult tissues. contrast, marsupial females show nonrandom activity, with repression Xp all somatic Here, we that MSCI also marsupials manner...
The gray, short-tailed opossum, Monodelphis domestica, is the most extensively used, laboratory-bred marsupial resource for basic biologic and biomedical research worldwide. To enhance utility of this species, we are building a linkage map, using both anonymous markers functional gene loci, that will enable localization quantitative trait loci (QTL) provide comparative information regarding evolution mammalian other vertebrate genomes. current map composed 83 distributed among eight...
Journal Article A CASE OF STRESS RELATED HETEROZYGOTE SUPERIORITY IN NATURE Get access Paul B. Samollow, Samollow Southwest Foundation for Research and Education P.O. Box 28147 San Antonio Texas 78284 Search other works by this author on: Oxford Academic Google Scholar Michael E. Soulé Institute Transcultural Studies 901 South Normandie Avenue Los Angeles California 90006 Evolution, Volume 37, Issue 3, 1 May 1983, Pages 646–649, https://doi.org/10.1111/j.1558-5646.1983.tb05584.x Published:...
The marsupial inactive X chromosome expresses a long noncoding RNA (lncRNA) called Rsx that has been proposed to be the functional analog of eutherian Xist . Despite possibility and encode related functions, two lncRNAs harbor no linear sequence similarity. However, both domains tandemly repeated sequence. In , these repeat are known critical for function. Using k -mer based comparison, we show unexpectedly partition into major clusters each substantial levels nonlinear Repeats B, C, D were...
Pulse pressure, a measure of aortic stiffness, is strong predictor cardiovascular mortality. To locate genes that affect pulse we performed genetic analysis on randomly ascertained families in the San Antonio Family Heart Study. pressure was defined as difference between systolic and diastolic blood pressures. Likelihood methods were used to construct model had both single-locus polygenic components for 46 (1308 individuals). The component included sex-specific genotype-specific effects age...
Abstract Essential hypertension has been linked to a highly polymorphic marker at the angiotensinogen locus, and association with polymorphism in this locus found some populations. We tested hypothesis that these same markers are essential Mexican Americans. The data comprised all affected relative pairs 46 extended families chosen random from low-income barrio San Antonio. Specifically, we searched for linkage by testing excessive alleles shared identical descent (IBD) among hypertensive...
Expression of X-linked glucose-6-phosphate dehydrogenase (G6PD) and phosphoglycerate kinase-A (PGK-A) in the Virginia opossum (Didelphis virginiana) was studied electrophoretically animals from natural populations those produced through controlled laboratory crosses. Blood most wild exhibited a common single-banded phenotype for both enzymes. Rare variant animals, regardless sex, phenotypes different mobility class respective enzyme. The crosses confirmed allelic basis rare phenotypes....